Electron Paramagnetic Resonance Spectrometer

电子顺磁共振波谱仪

• 批准号: 7590793
• 负责人: CECILIA GIULIVI
• 金额: $ 47.46万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7590793
• 关键词: Address; Affect; Aging; Agriculture; Alzheimer' s Disease; Antioxidants; Apoptosis; Area; Arts; Binding; Biochemistry; Biological; Biology; Biotechnology; Caloric Restriction; Carbon; Cell Respiration; Cells; Chemistry; Copper; Critical Care; Diabetes Mellitus; Disease; Electron Spin Resonance Spectroscopy; Engineering; Environmental Exposure; Faculty; Flavonoids; Free Radicals; Goals; Health; Human; Hypoxia; Immunology; Ischemia; Lung; Malaria; Malnutrition; Mechanics; Mediator of activation protein; Medicine; Microbiology; Molecular; Molecular Medicine; Myocardial Ischemia; Neurons; Nitric Oxide; Nitrogen; Nitrosation; Oxidants; Oxidation-Reduction; Oxidative Stress; Oxygen; Parasites; Play; Prevention; Production; Recruitment Activity; Reperfusion Therapy; Request for Proposals; Research; Role; Ryanodine Receptor Calcium Release Channel; Ryanodine Receptors; Sampling; Schools; Science; Signal Transduction Pathway; Slice; Surface; Tissues; Veterinary Medicine; Vision; Vitamin E; Whole Organism; Zinc deficiency; aqueous; college; design; improved; innovation; instrument; instrumentation; interest; multidisciplinary; nanoparticle; nanoscience; neoplastic; nitrosyl hemoglobin; nutrition; oxidation; public health relevance; research study; vector mosquito

DESCRIPTION (provided by applicant): The long-term goal of this proposal is to establish an Electron Spin Resonance (ESR) facility dedicated to biomedicine. The ESR Facility will address research at the interface between free radical biology/chemistry and medicine, a dynamic area that it has been intensifying extensively in the last 10 years. The Facility will be created on this vision and is designed to address the emerging research opportunities at the crossroad of multidisciplinary sciences. To this end, a multidisciplinary team of users and experts has been recruited from various Departments across Campus (Molecular Biosciences, Immunology & Microbiology, Biochemistry & Molecular Medicine, Biological Chemistry, Pulmonary & Critical Care, Mechanical & Aeronautical Engineering, and Nutrition), representing four different Colleges/Schools (Veterinary Medicine, Medicine, Agriculture, and Engineering) with interests in free radical medicine. Faculty will use this instrument to carry out research in several areas as follows: a) fundamental chemistry and biochemistry of antioxidants such as vitamin E, C and flavonoids; b) biochemistry of NO production in mosquito vectors of malaria parasites and the putative antiparasite role of nitrosylhemoglobin; c) role of the oxidation, S-nitrosation/S-glutathionylation in the ryanodine receptor type 1; d) nitric oxide as an important modulator of cell respiration and mediator of oxygen gradient redistribution; e) reactive oxygen and nitrogen radicals during caloric restriction and aging; f) copper and zinc deficiencies in free radical oxidations; g) radicals as markers of oxidative stress (e.g, ascorbyl, glutathionyl, and tocopheryl radicals); h) free radicals as initiators of apoptosis in neurons and neoplastic tissues; i) identification of bound radicals at the surface of nanoparticles, and how these affect health; j) free radicals in myocardial ischemia/reperfusion and temporary hypoxia. These studies will have an impact in a number of areas, from nanoscience to materials chemistry and biotechnology/biomedicine. This proposal requests the purchase of a state-of-the-art ESR spectrometer with capabilities for small, aqueous samples, cells, and tissue slices. By establishing such a Facility on Campus, we will provide the expertise and instrumentation to pursue research questions dealing with oxygen- and nitrogen-free radicals, carbon-centered radicals, related oxidants and antioxidants that influence the overall redox milieu of cells, tissues, and whole organisms. Biological relevant situations in which free radicals had been shown to play a critical role expand from signal transduction pathways to apoptosis, aging, diabetes, Alzheimer's, among other pathophysiological situations. Our primary goal is to advance the fundamental understanding of free radical biology/chemistry mechanisms so that human health can be improved through innovative approaches in the prevention and treatment of disease. PUBLIC HEALTH RELEVANCE: This proposal requests the purchase of an ESR spectrometer with capabilities for small, aqueous samples, cells, and tissue slices. By establishing such a Facility on Campus, we will provide the expertise and instrumentation to pursue research questions relevant to human medicine. The proposed experiments that only could be done using an ESR deal with the characterization of oxygen- and nitrogen-free radicals in a variety of medically relevant situations from aging, ischemia-reperfusion, and dietary deficiencies to environmental exposures.

描述（由申请人提供）：该提案的长期目标是建立专门用于生物医学的电子自旋共振（ESR）设施。 ESR 设施将致力于自由基生物学/化学与医学之间的交叉领域的研究，这是一个在过去 10 年中得到广泛加强的充满活力的领域。该设施将根据这一愿景创建，旨在解决多学科交叉领域的新兴研究机会。为此，从校园各个部门（分子生物科学、免疫学与微生物学、生物化学与分子医学、生物化学、肺与重症监护、机械与航空工程以及营养学）招募了一支由用户和专家组成的多学科团队，代表四个对自由基医学感兴趣的不同学院/学校（兽医、医学、农业和工程）。教师们将利用该仪器开展以下几个领域的研究： a) 维生素E、C和类黄酮等抗氧化剂的基础化学和生物化学； b) 疟疾寄生虫蚊媒中一氧化氮产生的生物化学以及亚硝酰血红蛋白推定的抗寄生虫作用； c) 1型兰尼碱受体中氧化、S-亚硝化/S-谷胱甘肽化的作用； d) 一氧化氮作为细胞呼吸的重要调节剂和氧梯度重新分布的调节剂； e) 热量限制和老化过程中的活性氧和氮自由基； f) 自由基氧化中铜和锌的缺乏； g) 作为氧化应激标记的自由基（例如抗坏血酸自由基、谷胱甘肽自由基和生育酚自由基）； h) 自由基作为神经元和肿瘤组织细胞凋亡的引发剂； i) 识别纳米粒子表面的结合自由基，以及它们如何影响健康； j) 心肌缺血/再灌注和暂时缺氧时的自由基。这些研究将对从纳米科学到材料化学和生物技术/生物医学的许多领域产生影响。该提案要求购买最先进的 ESR 光谱仪，能够处理小型水样、细胞和组织切片。通过在校园内建立这样的设施，我们将提供专业知识和仪器来解决涉及氧自由基和氮自由基、碳中心自由基、相关氧化剂和抗氧化剂的研究问题，这些问题影响细胞、组织和细胞的整体氧化还原环境。整个有机体。自由基已被证明发挥关键作用的生物学相关情况从信号转导途径扩展到细胞凋亡、衰老、糖尿病、阿尔茨海默病以及其他病理生理情况。我们的主要目标是增进对自由基生物学/化学机制的基本理解，以便通过预防和治疗疾病的创新方法来改善人类健康。公共健康相关性：该提案要求购买具有小型水样、细胞和组织切片功能的 ESR 光谱仪。通过在校园内建立这样的设施，我们将提供专业知识和仪器来探索与人类医学相关的研究问题。所提出的实验只能使用 ESR 来完成，涉及各种医学相关情况（从衰老、缺血再灌注、饮食缺乏到环境暴露）中氧自由基和氮自由基的表征。