Confocal Laser Scanning Biological Microscope Olympus Fv1000

共焦激光扫描生物显微镜 Olympus Fv1000

• 批准号: 7595715
• 负责人: SHU CHIEN
• 金额: $ 45.95万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595715
• 关键词: Address; Adhesions; Arts; Atherosclerosis; Biological; Biomedical Engineering; Cardiovascular system; Cartilage; Cells; Clinical; Data Set; Degenerative polyarthritis; Diagnosis; Diagnostic; Disease; Equipment; Faculty; Fluorescence Resonance Energy Transfer; Foundations; Goals; Grant; Health; Heart Diseases; Hypertension; Image; Investigation; Knowledge; Laser Scanning Confocal Microscopy; Lasers; Life; Malignant Neoplasms; Microcirculation; Microscope; Microscopic; Molecular; Organism; Pathogenesis; Prevention; Proteins; Regenerative Medicine; Research; Research Personnel; Research Project Grants; Resolution; Scanning; Signal Transduction; Signaling Molecule; Stem cells; Structure; System; Technology; Therapeutic; Time; Tissue Engineering; Tissues; Work; body system; design; fluorophore; frontier; innovation; instrument; instrumentation; meetings; member; nervous system disorder; neuron development; novel; novel strategies; programs; public health relevance; research study; stem cell biology; tool; trafficking

DESCRIPTION (provided by applicant): Recent advances in microscopic technologies provide powerful tools to visualize and quantify the molecular and cellular dynamics in living cells. Such novel microscopic tools are necessary for researchers to pursue their innovative projects at the frontier of bioengineering. In this proposal of Shared Instrumentation Grant, ten UCSD Bioengineering faculty members collectively request the purchase of an Olympus FV1000 Confocal Laser Scanning Biological Microscope System for the shared usage in their 15 projects in order to effectively carry out their research with state-of-the-art equipment to meet the research objectives in these grants. The research of the 15 projects is directed at four important fields: (a) Molecular and Cellular Signaling, (b) Stem Cells Bioengineering, (c) Tissue Engineering and Remodeling, and (d) Systems Bioengineering. These research projects are under the overarching theme of Integrative Bioengineering. Thus, our common scientific goal is integrative bioengineering, and the common technological need is the laser scanning confocal microscopy system. The current confocal microscope in UCSD Bioengineering was purchased 14 years ago; it is not only non-functional, but also lacks the features of the new system that are needed by the Project PIs to address the critical questions in their research programs and to initiate innovative investigations. In the Proposed Olympus FV1000 system, the long-time-lapse function of imaging multiple live cells will minimize laser damage for longer duration experiments and the multiple laser excitation wavelengths enable the acquisition of large data sets of multiple fluorophores. The 3D structural resolutions and temporal dynamics allow for high- quality imaging, including studies of the structures of matrices, cells, and tissues, as well as intracellular localization of marker molecules and trafficking. The FRET, FRAP, FLIM and TIRFM functions of the FV1000 are critical for dynamic studies of signaling molecules and adhesion proteins/matrix interactions. The research to be carried out with the use of this new share instrumentation will not only enhance our knowledge on the bioengineering foundation of structure and function of living systems in health, but also generate new approaches for the diagnosis, treatment and prevention of many health problems, such as cardiac diseases, hypertension, atherosclerosis, osteoarthritis, neurological diseases, and cancer. PUBLIC HEALTH RELEVANCE: The proposed instrument will be used to study the molecular and cellular dynamics in several body systems with an integrative approach. The work will address frontier problems in bioengineering research, including regenerative medicine, stem cell biology, cardiovascular dynamics, microcirculation, cartilage graft designs and neuronal development. The information generated from the research made possible by this instrument system will yield new knowledge on the pathogenesis of diseases and help to develop therapeutic and diagnostic tools for many important clinical conditions.

描述（由申请人提供）：微观技术的最新进展提供了可视化和量化活细胞中分子和细胞动力学的强大工具。这种新颖的微观工具对于研究人员在生物工程领域进行创新项目是必要的。在这项共享仪器赠款的建议中，十个UCSD生物工程教师共同要求购买15个项目中共享用法的Olympus FV1000共焦点激光扫描生物学显微镜系统，以便与先进的ART设备有效地进行研究，以实现这些赠款的研究目标。对15个项目的研究针对四个重要领域：（a）分子和细胞信号传导，（b）干细胞生物工程，（c）组织工程和重塑，以及（d）系统生物工程。这些研究项目是综合生物工程的总体主题。因此，我们的共同科学目标是整合生物工程，而常见的技术需求是激光扫描共聚焦显微镜系统。 14年前购买了UCSD生物工程的当前共聚焦显微镜；它不仅是非功能性的，而且还缺乏PIS项目所需的新系统的特征，以解决其研究计划中的关键问题并启动创新研究。在拟议的Olympus FV1000系统中，成像多个活细胞的长时间函数将在更长的持续时间实验中最大程度地减少激光损伤，而多个激光激发波长可以使大量数据集的多个荧光团的数据集获得。 3D结构分辨率和时间动力学允许进行高质量的成像，包括研究矩阵，细胞和组织的结构，以及标记分子和运输的细胞内定位。 FV1000的FRET，FRAP，FLIM和TIRFM功能对于信号分子和粘附蛋白/基质相互作用的动态研究至关重要。使用这种新共享仪器进行的研究不仅会增强我们对健康系统的结构和功能的生物工程基础的了解，而且还为诊断，治疗和预防许多健康问题（例如心脏疾病，高血压，高血压，高血压，动脉粥样硬化，动脉粥样硬化，骨关节炎，神经性疾病，神经性疾病和癌症）提供了新方法。公共卫生相关性：拟议的仪器将用于以综合方法的方式研究几种身体系统中的分子和细胞动力学。这项工作将解决生物工程研究中的前沿问题，包括再生医学，干细胞生物学，心血管动力学，微循环，软骨移植物设计和神经元发展。该仪器系统从研究中产生的信息将产生有关疾病发病机理的新知识，并有助于为许多重要的临床状况开发治疗和诊断工具。