A State-of-the-Art BIACORE T100 for UTMB

适用于 UTMB 的最先进的 BIACORE T100

• 批准号: 7595019
• 负责人: VINCENT J. HILSER
• 金额: $ 25.35万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595019
• 关键词: Advisory Committees; Applied Research; Arts; Award; Binding; Biological; Biology; Biophysics; Calorimetry; Communities; Conflict (Psychology); Development; Disease; Doctor of Philosophy; Grant; Hour; Housing; Kinetics; Laboratories; Measures; Medical; Medicine; Methods; Molecular; Online Systems; Process; Proteins; Publishing; Reaction; Research; Role; Sampling; Schedule; Solutions; Surface Plasmon Resonance; System; Techniques; Technology; Texas; Thermodynamics; Titrations; Training; Translational Research; Universities; design; drug development; insight; instrument; instrumentation; molecular recognition; physical process; public health relevance; structural biology

DESCRIPTION (provided by applicant): This award will provide partial support for the acquisition of a Biacore T100 surface plasmon resonance system at the University of Texas Medical Branch (UTMB) in Galveston. Molecular recognition is one of the most important problems in biology and medicine. This problem is not merely academic, since a clear understanding of the mechanism of recognition will facilitate the development of rational design strategies aimed at producing either agonistic or antagonistic modulators of therapeutically relevant proteins. Understanding molecular recognition requires technologies that provide a quantitative, mechanistic description of the binding process. Numerous methods have been developed to this end over the past 40 years, including isothermal titration calorimetry, and have provided significant insights into a broad range of phenomena. However, no single technique provides the combination of capabilities available using the Biacore T100 surface plasmon resonance system. This instrument provides automated, high-throughput capabilities with high sensitivity, requires only low sample quantities, and can provide a complete thermodynamic as well as kinetic characterization of the binding process. This combination of features is perfectly suited to the broad spectrum of applications, both basic and translational, described in this proposal. The requested instrument will be housed, maintained, and operated in the Solution Biophysics Core Laboratory of the Sealy Center for Structural Biology and Molecular Biophysics. The PhD-level manager of this Core has already received training from Biacore; he and the major users will receive additional training from Biacore after purchase. Scheduling of the instrument will be web-based, and any conflicts will be resolved by an established advisory committee that includes the PI of the grant and major users. Approximately 15% of the available hours will be reserved for new and outside users. The ability of this technology to impact both the basic and applied research efforts described in this proposal portends a major role for the Biacore T100 in UTMB's translational research efforts. The fact that each of the primary users has been able to generate either published or preliminary results, despite the current very limited access to SPR instrumentation on campus, demonstrates the UTMB research community's commitment to this technology. PUBLIC HEALTH RELEVANCE: Molecular recognition is the physical process underlying all of biology and medicine, and technologies that provide a rapid and accurate characterization of the recognition process are fundamental to an understanding of diseases, as well as to the development of drugs to treat them. The instrument requested in this grant, the BIACORE T100 surface plasmon resonance system, provides a state-of-the-art means of measuring binding reactions for a diverse set of biological problems, and will represent a core technology in UTMB's translational research efforts.

描述（由申请人提供）：该奖项将为在Galveston的德克萨斯大学医学分公司（UTMB）收购Biacore T100表面等离子体共振系统提供部分支持。分子识别是生物学和医学中最重要的问题之一。这个问题不仅是学术，因为对识别机制的清晰了解将有助于制定旨在产生治疗相关蛋白质的激动或拮抗调节剂的理性设计策略。理解分子识别需要提供结合过程的定量机械描述的技术。在过去的40年中，已经开发了许多方法，包括等温滴定量热法，并为广泛的现象提供了重要的见解。但是，没有单个技术可以使用BIACORE T100表面等离子体共振系统提供功能的组合。该仪器提供具有高灵敏度的自动化，高通量功能，仅需要较低的样品数量，并且可以提供完整的热力学和动力学表征。这种特征的组合非常适合本提案中描述的基本和转化的广泛应用。所请求的仪器将在Sealy结构生物学和分子生物物理学中心的溶液生物物理学核心实验室中容纳，维护和操作。该核心的博士学位经理已经接受了Biacore的培训。他和主要用户购买后将获得Biacore的额外培训。该工具的安排将是基于网络的，任何冲突将由包括赠款和主要用户的PI在内的既定咨询委员会解决。可用小时的大约15％将保留给新用户和外部用户。该技术影响该提案中描述的基本研究工作和应用研究工作的能力预示了Biacore T100在UTMB的转化研究工作中的主要作用。尽管目前在校园中对SPR仪器的访问非常有限，但每个主要用户都能够产生已发布或初步结果，这一事实证明了UTMB研究社区对这项技术的承诺。 公共卫生相关性：分子识别是所有生物学和医学的物理过程，以及提供识别过程快速准确表征的技术，是对疾病的理解以及对治疗药物的发展的基础。该赠款中要求的仪器是Biacore T100表面等离子体共振系统，提供了一种测量各种生物学问题的结合反应的最新方法，并将代表UTMB转化研究工作中的核心技术。