A State-of-the-Art BIACORE T100 for UTMB

适用于 UTMB 的最先进的 BIACORE T100

• 批准号: 7595019
• 负责人: VINCENT J. HILSER
• 金额: $ 25.35万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595019
• 关键词: Advisory Committees; Applied Research; Arts; Award; Binding; Biological; Biology; Biophysics; Calorimetry; Communities; Conflict (Psychology); Development; Disease; Doctor of Philosophy; Grant; Hour; Housing; Kinetics; Laboratories; Measures; Medical; Medicine; Methods; Molecular; Online Systems; Process; Proteins; Publishing; Reaction; Research; Role; Sampling; Schedule; Solutions; Surface Plasmon Resonance; System; Techniques; Technology; Texas; Thermodynamics; Titrations; Training; Translational Research; Universities; design; drug development; insight; instrument; instrumentation; molecular recognition; physical process; public health relevance; structural biology

DESCRIPTION (provided by applicant): This award will provide partial support for the acquisition of a Biacore T100 surface plasmon resonance system at the University of Texas Medical Branch (UTMB) in Galveston. Molecular recognition is one of the most important problems in biology and medicine. This problem is not merely academic, since a clear understanding of the mechanism of recognition will facilitate the development of rational design strategies aimed at producing either agonistic or antagonistic modulators of therapeutically relevant proteins. Understanding molecular recognition requires technologies that provide a quantitative, mechanistic description of the binding process. Numerous methods have been developed to this end over the past 40 years, including isothermal titration calorimetry, and have provided significant insights into a broad range of phenomena. However, no single technique provides the combination of capabilities available using the Biacore T100 surface plasmon resonance system. This instrument provides automated, high-throughput capabilities with high sensitivity, requires only low sample quantities, and can provide a complete thermodynamic as well as kinetic characterization of the binding process. This combination of features is perfectly suited to the broad spectrum of applications, both basic and translational, described in this proposal. The requested instrument will be housed, maintained, and operated in the Solution Biophysics Core Laboratory of the Sealy Center for Structural Biology and Molecular Biophysics. The PhD-level manager of this Core has already received training from Biacore; he and the major users will receive additional training from Biacore after purchase. Scheduling of the instrument will be web-based, and any conflicts will be resolved by an established advisory committee that includes the PI of the grant and major users. Approximately 15% of the available hours will be reserved for new and outside users. The ability of this technology to impact both the basic and applied research efforts described in this proposal portends a major role for the Biacore T100 in UTMB's translational research efforts. The fact that each of the primary users has been able to generate either published or preliminary results, despite the current very limited access to SPR instrumentation on campus, demonstrates the UTMB research community's commitment to this technology. PUBLIC HEALTH RELEVANCE: Molecular recognition is the physical process underlying all of biology and medicine, and technologies that provide a rapid and accurate characterization of the recognition process are fundamental to an understanding of diseases, as well as to the development of drugs to treat them. The instrument requested in this grant, the BIACORE T100 surface plasmon resonance system, provides a state-of-the-art means of measuring binding reactions for a diverse set of biological problems, and will represent a core technology in UTMB's translational research efforts.

描述（由申请人提供）：该奖项将为加尔维斯顿德克萨斯大学医学分校 (UTMB) 购买 Biacore T100 表面等离子共振系统提供部分支持。分子识别是生物学和医学中最重要的问题之一。这个问题不仅仅是学术问题，因为对识别机制的清晰理解将有助于开发合理的设计策略，旨在产生治疗相关蛋白质的激动或拮抗调节剂。了解分子识别需要能够对结合过程进行定量、机械描述的技术。过去 40 年来，为此开发了许多方法，包括等温滴定量热法，并为广泛的现象提供了重要的见解。然而，没有任何一种技术能够提供使用 Biacore T100 表面等离子共振系统的可用功能的组合。该仪器提供自动化、高通量功能和高灵敏度，仅需要少量样品，并且可以提供结合过程的完整热力学和动力学表征。这种功能组合非常适合本提案中描述的广泛应用，包括基本应用和转化应用。所需的仪器将在西利结构生物学和分子生物物理学中心的解决方案生物物理学核心实验室中安置、维护和操作。该Core的博士级管理者已经接受过Biacore的培训；购买后，他和主要用户将接受Biacore的额外培训。该工具的安排将基于网络，任何冲突都将由一个既定的咨询委员会解决，该委员会包括赠款的 PI 和主要用户。大约 15% 的可用时间将保留给新用户和外部用户。该技术能够影响本提案中描述的基础和应用研究工作，这预示着 Biacore T100 在 UTMB 的转化研究工作中将发挥重要作用。尽管目前校园内 SPR 仪器的使用非常有限，但每个主要用户都能够生成已发布的或初步的结果，这一事实表明了 UTMB 研究界对这项技术的承诺。 公共健康相关性：分子识别是所有生物学和医学的基础物理过程，提供快速、准确表征识别过程的技术对于了解疾病以及开发治疗疾病的药物至关重要。本次拨款申请的仪器是 BIACORE T100 表面等离子共振系统，它提供了一种最先进的测量各种生物问题结合反应的方法，并将代表 UTMB 转化研究工作的核心技术。