LabMaster: food and water intake, activity and metabolic rate

LabMaster：食物和水的摄入量、活动和代谢率

• 批准号: 7595323
• 负责人: DANIELLE Renee REED
• 金额: $ 26.74万
• 依托单位: MONELL CHEMICAL SENSES CENTER
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-03 至 2010-05-02
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595323
• 关键词: Adult; Alcohol Phenotype; Alcoholism; Animal Model; Animals; Area; Behavior; Biomedical Research; Body Weight; Breeding; Calcium; Carbon Dioxide; Chemicals; Data; Desire for food; Diabetes Mellitus; Disease; Eating; Energy Metabolism; Equipment; Equipment Design; Food; Food Patterns; Funding; Gases; Genetic; Genetically Engineered Mouse; Inflammation; Institution; Intake; Learning; Measurement; Measures; Metabolic; Metabolism; Minerals; Modeling; Monitor; Mouse Strains; Mus; Nausea; Nutrient; Obesity; Osteoporosis; Oxygen Consumption; Pharmaceutical Preparations; Phenotype; Production; Research; Research Personnel; Rodent; System; Taste Perception; Translational Research; United States National Institutes of Health; Water consumption; Work; genetic manipulation; human disease; lipid metabolism; obesity prevention; preference; public health relevance; relating to nervous system; trait

DESCRIPTION (provided by applicant): In biomedical research using animal models such as mice, it is often useful and sometimes essential to understand the animal's metabolic status in different experimental states, for example, before and after drug treatment or as a result of genetic manipulation, selective breeding, or learning. In that spirit, this application focuses on the need of 10 National Institutes of Health-funded projects by seven investigators from the Monell Chemical Senses Center to obtain equipment designed to automatically measure food and water intake, activity, oxygen consumption and carbon dioxide production. The advantage of this system is that it measures key aspects of metabolism of rodents continuously and accurately, which aids in the interpretation and implementation of a variety of studies. No such equipment is currently available within the Monell Chemical Senses Center, an independent, nonprofit research institution. The projects that would benefit from an automated and accurate monitoring of these phenotypes in mice (and other models) relate to the genetics of obesity; the effects of early husbandry on adult phenotypes; alcohol preference behavior among selectively bred strains of mice; the interaction of taste and obesity, food intake, and body weight, especially in mice that are "sweet-likers"; treatments specifically targeted at changing dietary composition and fat metabolism; the neural concomitants of nausea and learned taste aversions; the intake and appetite for minerals such as calcium; the effect of inflammation on taste; and genetically engineered mice with compromised or enhanced taste function. This equipment is needed by a large core group of investigators and will enhance the quality of the work done here. Currently, food and water intake data are collected manually, and there are no facilities for the measurement of activity or gas exchange. Translational research in these areas is of critical concern because of the focus on obesity prevention and other diseases of nutrient intake, e.g., alcoholism, osteoporosis, excess sweet intake, and diabetes. PUBLIC HEALTH RELEVANCE: The pattern of food and water intake, activity, and metabolism in mice changes as a result of genetic manipulation or other experimental treatments. The accurate and detailed measurement of these traits in experimental animals enhances the quality and reliability of experimental results. To that end, we are requesting funds to purchase a system that will automatically and continuously measure food and water intakes, activity, and energy metabolism of rodents used as models of human disease.

描述（由申请人提供）：在使用小鼠等动物模型的生物医学研究中，了解动物在不同实验状态下的代谢状态通常是有用的，有时是必要的，例如，药物治疗之前和之后或基因操作的结果、选择性育种或学习。本着这种精神，本申请重点关注莫内尔化学感官中心七名研究人员的 10 个美国国立卫生研究院资助项目的需求，以获得旨在自动测量食物和水摄入量、活动、耗氧量和二氧化碳产生的设备。该系统的优点是它可以连续、准确地测量啮齿类动物新陈代谢的关键方面，这有助于解释和实施各种研究。莫奈尔化学感官中心（一家独立的非营利性研究机构）目前没有此类设备。这些项目将受益于对小鼠（和其他模型）中这些表型的自动和准确监测，这些表型与肥胖遗传学有关；早期饲养对成年表型的影响；选择性繁殖的小鼠品系的酒精偏好行为；味觉与肥胖、食物摄入量和体重的相互作用，尤其是“喜欢甜食”的小鼠；专门针对改变饮食成分和脂肪代谢的治疗；恶心和习得性味觉厌恶的神经伴随物；钙等矿物质的摄入量和食欲；炎症对味觉的影响；以及味觉功能受损或增强的基因工程小鼠。该设备是大量核心调查人员所需要的，并将提高这里所做工作的质量。目前，食物和水摄入量数据是手动收集的，并且没有用于测量活动或气体交换的设施。这些领域的转化研究备受关注，因为重点是预防肥胖和其他营养摄入疾病，例如酗酒、骨质疏松症、甜食摄入过多和糖尿病。 公共卫生相关性：由于基因操作或其他实验治疗，小鼠的食物和水摄入、活动和代谢模式发生变化。对实验动物这些性状的准确和详细测量可以提高实验结果的质量和可靠性。为此，我们正在申请资金购买一个系统，该系统将自动连续测量用作人类疾病模型的啮齿动物的食物和水摄入量、活动和能量代谢。