A Diagnostic Microarray for hemorrhagic fever encephalitis viruses

出血热脑炎病毒的诊断微阵列

基本信息

批准号：
7919406
负责人：
DAVID WANG
金额：
$ 78.48万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-01 至 2012-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7919406
关键词：
Adenoviruses Algorithms Alphavirus Animal Model Animals Arboviruses Area Arenaviridae Bioinformatics Biological Assay Biopsy Bioterrorism Brain Bunyaviridae Canada Centers for Disease Control and Prevention (U.S.)Characteristics Clinical Clinical Virology Collaborations Collection Computational algorithm Conserved Sequence Containment Containment of Biohazards Coronavirus DNA Microarray Chip Data Detection Development Diagnosis Diagnostic Diagnostic Procedure Diagnostic tests Disease Disease Outbreaks Elements Encephalitis Encephalitis Viruses Engineering Enterovirus Etiology Family Flaviviridae Frankfurt-Marburg Syndrome Virus Genetic Recombination Goals Health Hepatitis A Hour Human Human Herpesvirus 8 In Vitro Individual Infection Influenza Label Laboratories Laboratory Research Lassa fever virus Life Measles Metapneumovirus Microbiology Molecular Nucleic Acid Amplification Tests Nucleic Acid Probes Nucleic Acids Oligonucleotides Output Parainfluenza Patients Pattern Pediatric Hospitals Performance Postdoctoral Fellow Poxviridae Preparation Process Public Health Qualifying Readiness Reassortant Viruses Recombinants Research Research Personnel Respiratory syncytial virus Rhinovirus Rotavirus SARS coronavirus Sampling Satellite Viruses Scanning Scheme Severe Acute Respiratory Syndrome Source Specimen Syndrome System Taxonomy Technology Textbooks Time Universities Validation Variant Viral Viral Genome Viral Hemorrhagic Fevers Viremia Virus Washington Work animal tissue base biosecurity design experience hemorrhagic fever virus improved interest meetings microbial novel pathogen portability programs rapid diagnosis recombinant virus reconstitution research study viral detection virology virus culture virus development

项目摘要

DESCRIPTION (provided by applicant): Hemorrhagic fever and encephalitis are both devastating clinical syndromes that can be caused by a large number of microbial agents. Some of the causative agents of these diseases have the potential to be weaponized or used as bioterrorist agents. Rapid diagnosis of the etiologic agent is critical to distinguish natural occurring cases from potential acts of bioterrorism. The goal of this project is to develop and validate an integrated diagnostic platform for detection of all viruses known to cause encephalitis and hemorrhagic fevers. The core of this technology combines a DNA microarray, containing a set of nucleic acid probes capable of hybridizing to essentially all known viruses, with a computational algorithm for calling the presence or absence of viruses in statistical fashion. In this application a DNA microarray capable of species-specific identification of all viruses associated with these diseases will be designed. The ability of this platform to detect multiple strains of each known virus using cultured viruses, spiked clinical samples and authentically infected clinical specimens will be explicitly validated. Furthermore, detection limits will be quantified and the ability of the platform to unambiguously characterize reassortant, recombinant and engineered viruses will be validated. Finally, the portability of the diagnostic assay will be evaluated by assessing the performance of the microarray diagnostic assay in a clinical setting. Development of improved diagnostic methods for viral detection, especially for potential bioterrorism agents, will dramatically increase public health preparedness. The goal of this project is to develop and validate a novel DNA microarray based diagnostic assay for viruses that cause hemorrhagic fever and encephalitis.

描述（由申请人提供）：出血热和脑炎都是由大量微生物引起的破坏性临床综合征。这些疾病的一些病原体有可能被武器化或用作生物恐怖分子。病原体的快速诊断对于区分自然发生的病例和潜在的生物恐怖主义行为至关重要。该项目的目标是开发和验证一个综合诊断平台，用于检测所有已知引起脑炎和出血热的病毒。这项技术的核心是将DNA微阵列（包含一组能够与基本上所有已知病毒杂交的核酸探针）与计算算法相结合，以统计方式判断病毒是否存在。在此应用中，将设计能够对与这些疾病相关的所有病毒进行物种特异性鉴定的DNA微阵列。该平台使用培养病毒、加标临床样本和真正感染的临床样本检测每种已知病毒的多种毒株的能力将得到明确验证。此外，检测限将被量化，并且平台明确表征重配、重组和工程病毒的能力将得到验证。最后，将通过评估微阵列诊断测定在临床环境中的性能来评估诊断测定的可移植性。开发改进的病毒检测诊断方法，特别是针对潜在的生物恐怖主义制剂，将极大地提高公共卫生准备工作。该项目的目标是开发和验证一种新型 DNA 微阵列诊断方法，用于检测引起出血热和脑炎的病毒。