Cell Culture and Engineering Core (CCEC)

细胞培养与工程核心（CCEC）

基本信息

批准号：
10693936
负责人：
OWEN M WOODWARD
金额：
$ 18.72万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-24 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10693936
关键词：
3-Dimensional Academia Acceleration Address Affect Alleles Antibodies Autosomal Dominant Polycystic Kidney Biological Assay Cell Culture Techniques Cell Line Cell model Cells Cellular Assay Clinical Clinical Data Collaborations Communities Consultations Coupled Custom Cyst Cyst Fluid Data Duct (organ) structure Ductal Epithelial Cell Engineering Ensure Enterobacteria phage P1 Cre recombinase Epithelial Cells Experimental Designs Future Gene Expression Gene Expression Profiling Gold Government Human In Vitro Individual Industry Investigation Kidney Knock-out Knockout Mice LoxP-flanked allele Maryland Modeling Mus Mutation Organoids Pathway interactions Patients Phenotype Polycystic Kidney Diseases Positioning Attribute Preparation Primary Cell Cultures Procedures Proliferating Proprotein Convertase 1 Protocols documentation Quality Control Reproducibility Research Research Personnel Resources Services Site Specimen Stretching System Tetracyclines Therapeutic Tissues Translations Validation cellular engineering design frontier high standard high throughput screening in vitro Model inducible Cre innovation interest kidney epithelial cell knock-down mutant new technology organizational structure renal epithelium screening single nucleus RNA-sequencing therapeutic evaluation treatment response validation studies vector

项目摘要

PROJECT SUMMARY The Maryland Polycystic Kidney Disease Research and Translation Core Center (MPKD-RTCC) Cell Culture and Engineering Core (CCEC) will accelerate progress in PKD research by addressing a major unmet need for well-defined, reliable in vitro cell PKD models with proper controls. With this overarching goal in mind, CCEC developed new in vitro cell and organoid models that we hope will become the “gold-standards” of the field. The CCEC is committed to helping investigators find ideal solutions for all their individual in vitro research needs whether it involves mechanistic exploration, human PKD cell validation studies, or therapeutic screening. To best serve the current and expected future demands of the diverse PKD workforce in academia, government, and industry, the CCEC will provide murine cell models of ADPKD, provide engineered inner medullary collecting duct (IMCD3) cell lines expressing custom PKD1 and PKD2 mutations, provide human kidney ADPKD cells and specimens, provide a cutting edge cystogenesis assay using three-dimensional organoid system, provide high through-put, three- dimensional cyst expansion assay, and provide consultation services to make sure the PKD community can fully utilize the CCEC resources.

项目概要马里兰州多囊肾病研究与转化核心中心 (MPKD-RTCC) 细胞培养与工程核心 (CCEC) 将通过以下方式加速 PKD 研究的进展解决对明确定义的、可靠的体外细胞 PKD 模型的主要未满足需求考虑到这一总体目标，CCEC 开发了新的体外细胞和类器官。我们希望这些模型能够成为该领域的“黄金标准”。帮助研究人员找到满足其所有个人体外研究需求的理想解决方案，无论是涉及机制探索、人类 PKD 细胞验证研究或治疗筛选。最好地满足学术界多元化 PKD 劳动力当前和预期的未来需求， CCEC 将提供 ADPKD 的小鼠细胞模型，提供表达定制 PKD1 和 PKD2 的工程内髓集合管 (IMCD3) 细胞系突变，提供人肾ADPKD细胞和标本，提供前沿使用三维类器官系统进行囊肿发生测定，提供高通量、三三维囊肿扩张试验，并提供咨询服务以确保PKD 社区可以充分利用CCEC资源。