FLOW CYTOMETRY CORE

流式细胞术核心

• 批准号: 7622568
• 负责人: AMY JO WAGERS
• 金额: $ 20.91万
• 依托单位: JOSLIN DIABETES CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7622568
• 关键词: Biomedical Research; Cells; Complex; Core Facility; Data Analyses; Deposition; Detection; Development; Dissection; Elements; Flow Cytometry; Fluorescence; Gene Expression Regulation; Goals; Immunology; Individual; Islets of Langerhans; Molecular Biology; Numbers; Population; Preparation; Proteins; Purpose; Research Personnel; Sampling; Schedule; Signaling Molecule; Sorting - Cell Movement; Speed; Stem cells; Surface; Technology; Time; Training and Education; Update; cell behavior; cost; instrument; instrumentation; new technology; response; technology development

Flow cytometry has become an essential element of biomedical research in a number of fields beyond immunology, where it originated in the 1970s. Continuous advances in instrumentation have increased the scope of flow cytometry and expanded the types of analyses made possible by this technology. The simultaneous detection of up to 12 fluorescence parameters allows exquisite dissection of cell states, including analysis at the single-cell level of surface expressed proteins, intracellular signaling molecules, and certain enzymatic activities. In addition, sorting at speeds of up to 30,000 cells/sec enables the direct isolation and characterization of even very rare cell populations (present at <0.01 % of total cells). Single cell deposition permits clonal analysis of cell behavior and the application of molecular biology approaches to individual cells of a population, providing a richer and more informative description of gene regulation and responsiveness at the single-cell level. Finally, the recent development of large-object sorting brings the ability to distinguish and analyze complex cell clusters, such as differentiating stem cells or pancreatic islets. Joslin's Flow Cytometry Core Facility has for some time provided Center researchers with an advanced facility for complex, multi-purpose cell analysis and sorting by flow cytometry. The goals of the Core are: 1. To implement and maintain a reliable, multi-functional and cost-efficient Flow Cytometry facility with streamlined operator support to minimize scheduling bottlenecks and machine down-time. 2. To provide education and training to Joslin researchers regarding potential applications of flow cytometry and intricate aspects of sample preparation/handling and of data analysis. 3. To continually update instrumentation in response to new and cutting-edge technology developments, including upgrades to existing instruments, addition of new instrumentation, and establishment of collaborative arrangements to help develop and evaluate new technology.

流式细胞仪已成为许多领域生物医学研究的基本要素 免疫学，起源于1970年代。仪器的持续进展增加了 流式细胞术的范围并扩大了该技术使得分析的类型。这 同时检测多达12个荧光参数可以使细胞态的精美解剖， 包括在表达蛋白的单细胞水平上分析，细胞内信号分子和 某些酶活性。此外，以高达30,000个单元/秒的速度排序可以直接排序 甚至非常罕见的细胞群体的分离和表征（占总细胞的<0.01％）。单身的 细胞沉积允许对细胞行为的克隆分析以及分子生物学方法的应用 人群的个别细胞，提供对基因调节的更丰富，更有信息的描述 单细胞水平的响应能力。最后，大型对象分类的最新发展带来了 能够区分和分析复杂细胞簇的能力，例如区分干细胞或胰岛。 乔斯林的流式细胞仪核心设施已有一段时间为中心研究人员提供了高级 用于复杂的多用途细胞分析和通过流式细胞术进行分类的设施。核心的目标是：1。 使用和维护可靠，多功能和成本效益的流式细胞仪设施 简化操作员的支持，以最大程度地减少调度瓶颈和机器停机时间。 2。向乔斯林研究人员提供有关流式细胞仪的潜在应用的教育和培训 样本制备/处理和数据分析的复杂方面。 3。为了响应新的和尖端的技术发展，不断更新仪器， 包括升级到现有工具，增加新仪器以及建立 协作安排，以帮助开发和评估新技术。