Biopolymer Shared Service

生物聚合物共享服务

基本信息

批准号：
7696605
负责人：
Nicholas P Ambulos
金额：
$ 5.32万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-08-08 至 2011-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7696605
关键词：
Accounting Antisense Oligonucleotides Arts Biopolymers Blood capillaries Calibration Cancer Center Commit Complex Computers and Advanced Instrumentation Cyclic Peptides DNA Development Effectiveness Environment Faculty Fluorescence Resonance Energy Transfer Fostering Funding Gene Expression Genome Genotype Grant Hour Institutes Investigation Label Malignant Neoplasms Maryland Measurable Methodology Mission Molecular Molecular Probes Mutation National Cancer Institute Numbers Oligonucleotides Peptide Synthesis Peptides Pharmaceutical Preparations Polymers Publications RNA chemical synthesis Reference Standards Research Research Personnel Resources Science Services Standards of Weights and Measures Students System Technology Time Training Universities Validation base capillary cost falls genome wide association study innovative technologies instrument instrumentation medical specialties member next generation novel programs success synthetic peptide

项目摘要

Biopolymer Shared Service The Biopolymer Shared Service (SS) uses state-of-the-art instrumentation to support molecular biologybased research through services that include DNA/RNA synthesis, DMA sequencing, genotyping, and peptide synthesis. The facility provides these services to the entire University of Maryland campus. The University of Maryland Marlene and Stewart Greenebaum Cancer Center's (UMGCC's) membership accounts for about one fourth of the facility's use. The Biopolymer SS synthesizes oligonucleotides with a rapid 24-hour turnaround time. The facility also routinely synthesizes specialty oligonucleotides, such as SiRNA, and dual-labeled fluorescence resonance energy transfer probes, molecular beacons, and antisense oligonucleotides in a rapid, cost-effective manner. The addition of a 96-capillary array DNA sequencer (ABI 3730XL) to the facility has greatly enhanced this service, decreasing chargeback costs and turnaround time and increasing throughput. An Affymetrix GeneChip System 3000 7G supports microarray-based expression and genome-wide association studies. The use of synthetic peptides in this research environment has expanded through the facility's ability to provide modified peptides, such as cyclic peptides, via multiple methodologies, labeled peptides, and complex multibranched polymers. The Biopolymer SS will incorporate an Illumina (formerly Solexa) Genome Analyzer in fall 2007, with significant support from UMGCC. This next-generation sequencer will enhance the capabilities to detect novel, cancer-associated mutations and provide a unique platform for determining changes in gene expression. Additionally, the Biopolymer SS embodies an intellectual reservoir for the development of innovative technology. The facility's partnership with investigators greatly enhances its research programs. The shared service has also developed novel peptide standards in conjunction with the National Institute of Standards and Technology; these will serve as standard reference material for drug validation and instrument calibration. The facility has received competitive shared instrumentation grants, allowing it to provide highly effective support of cutting-edge research with state-of-the-art instrumentation. This effectiveness is measurable by the large number of publications that the facility has supported: more than 100 publications per year from members of UMGCC alone. Many of these publications focus on investigations funded by the National Cancer Institute.

生物聚合物共享服务生物聚合物共享服务（SS）使用最先进的仪器来支持基于分子生物学的仪器通过包括DNA/RNA合成，DMA测序，基因分型和肽合成。该设施为整个马里兰大学校园提供了这些服务。这马里兰州玛琳大学和斯图尔特·格林鲍姆癌症中心（UMGCC）会员资格约占该设施使用的四分之一。生物聚合物SS以24小时的周转时间快速合成寡核苷酸。设施也是如此常规合成特种寡核苷酸，例如siRNA和双标记的荧光共振能量转移探针，分子信标和反义寡核苷酸快速，成本效益方式。将96个毛细管阵列DNA Sequencer（ABI 3730xl）添加到该设施中增强了这项服务，降低了退款成本和周转时间以及增加的吞吐量。一个 Affymetrix Genechip System 3000 7G支持基于微阵列的表达和全基因组关联研究。在该研究环境中的合成肽的使用已通过该设施的能够通过多种方法，标记的肽，提供改良的肽，例如环状肽，和复杂的多型聚合物。生物聚合物SS将结合一个Illumina（以前为Solexa） 2007年秋季的基因组分析仪，在UMGCC的大力支持下。下一代的音序器将增强检测新颖的，与癌症相关的突变的能力，并为确定基因表达的变化。此外，生物聚合物SS体现了创新发展的知识分子库技术。该设施与研究人员的合作关系大大增强了其研究计划。共享服务还与国家标准研究所共同开发了新颖的肽标准和技术；这些将用作药物验证和仪器的标准参考材料校准。该设施已获得有竞争力的共享仪器赠款，使其能够高度提供最先进的仪器对尖端研究的有效支持。这种有效性是该设施支持的大量出版物可衡量：100多个出版物仅UMGCC成员每年。这些出版物中的许多侧重于由国家癌症研究所。