EMBRYONIC STEM CELL AND EMBRYONIC GERM CELL LINE

胚胎干细胞和胚胎生殖细胞系

• 批准号: 7916439
• 负责人: Roger Arnold Pedersen
• 金额: $ 64.17万
• 依托单位: MAGEE-WOMEN'S RES INST AND FOUNDATION
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7916439
• 关键词: Aliquot; Aneuploidy; Animal Model; Animals; Back; Cell Count; Cell Culture Techniques; Cell Differentiation process; Cell Line; Cell surface; Cells; Chimera organism; Cryopreservation; DNA Fingerprinting; Development; Disease; EG Cell Line; Embryo; Ensure; Epigenetic Process; Extracellular Matrix; Fluorescent in Situ Hybridization; Freezing; Generations; Genetic; Germ Cells; Human; Human Development; Human Resources; In Vitro; Karyotype; Laboratories; Measures; Molecular; Monitor; Pluripotent Stem Cells; Preparation; Primary Cell Cultures; Principal Investigator; Property; Quality Control; Research Personnel; Resources; Serum; Stem cells; Techniques; Transplantation; Xenograft procedure; base; embryonic stem cell; good laboratory practice; human embryonic stem cell; improved; in vivo; nonhuman primate; pluripotency; quality assurance; scale up; stem; sterility testing; telomere; web site

Each of the principal investigators for projects I-III is a recognized expert in human and non-human primate embryonic stem and germ cells. The Embryonic Stem Cell and Embryonic Germ Cell ("Stem and Germ Cell") Core draws on this combined expertise, thereby providing a central quality assurance and quality control laboratory for all cellular resources generated by the three projects. Three specific aims are proposed for this core in support of the three projects. Aim 1: Establish and maintain core resource of human and non-human primate embryonic stem cells for distribution to each of the projects and the other cores using good laboratory practice culture and frequent monitoring of pluripotency and aneuploidy. Aim 2: Establish and maintain core resource of non-human primate embryonic germ cells for distribution to each of the projects and the other cores using good laboratory practice culture and frequent monitoring of pluripotency and aneuploidy. Aim 3 (Stem Cell Genetic and Epigenetic Resource) Maintain sets of human and nonhuman primate ES and EG cell lines that are well-characterized with respect to their genetic and epigenetic status (whether normal or abnormal) for use in each of the projects and the other cores. The fulfillment of each of the three aims will support the three projects and the other cores in providing for in vitro molecular and cellular analysis, generation of chimeras (in the case of non-human primate ES and EG cells); and analysis of developmental fates, both in vitro and in vivo (the latter in transplantation studies involving animal models). By combining the expertise and resources of the three project leaders this core maximizes the available resources and reduces duplication and overlap.

I-III项目的每个主要研究人员都是人类和非人类灵长类动物胚胎的公认专家 茎和生殖细胞。胚胎干细胞和胚胎生殖细胞（“茎和生殖细胞”）核心借助 联合专业知识，从而为所有蜂窝提供了中央质量保证和质量控制实验室 这三个项目产生的资源。为支持这三个项目提供了三个具体目标。 目标1：建立和维护人类和非人类灵长类动物胚胎干细胞的核心资源，以分布到 每个项目和其他核心都使用良好的实验室实践文化以及对多能的频繁监测 和非整倍性。目标2：建立并维护非人类灵长类动物胚胎生殖细胞的核心资源 使用良好的实验室实践文化向每个项目和其他核心分发 多能性和非整倍性。 AIM 3（干细胞遗传和表观遗传资源）保持人类和非人类的集合 灵长类动物ES和EG细胞系相对于其遗传和表观遗传状态进行了很好的特征 （无论是正常还是异常）用于每个项目和其他核心。这三个中的每一个 目标将支持三个项目和其他核心，以提供体外分子和细胞分析，即 嵌合体的产生（对于非人类灵长类动物ES和EG细胞）；以及对发展命运的分析 体外和体内（后者的移植研究涉及动物模型）。通过结合专业知识和 这三个项目负责人的资源这核心最大化可用资源，并减少重复和重叠。