Protective film-forming disinfectant based on chitosan/water/ethanol tertiary solutions

基于壳聚糖/水/乙醇三元溶液的保护膜消毒剂

• 批准号: 10662022
• 负责人: Alexey A Vertegel
• 金额: $ 5.5万
• 依托单位: VRM LABS INC
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-13 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10662022
• 关键词: 2019-nCoV; Affect; Aftercare; Alcohols; American; Ammonium; Antifungal Agents; Antiviral Agents; Beds; COVID-19; COVID-19 pandemic; COVID-19 patient; COVID-19 treatment; Centers for Disease Control and Prevention (U.S.); Cessation of life; Chitosan; Coagulants; Copper; Coronavirus; Country; Crustacea; Disinfectants; Disinfection; Elevator; Environment; Environmental Protection; Ethanol; Feedback; Film; Food Additives; Formulation; Goals; Growth; Guidelines; Hand; Health; Home; Hospitals; Hour; Household; Human; Hydrogen Peroxide; Hygiene; Hypochlorite; In Vitro; Infection; Manufactured Materials; Measures; Medical; Microbiology; Modeling; Modernization; Outcome; Patients; Performance; Persons; Polymers; Population; Prevention; Preventive; Property; Protocols documentation; Recommendation; Resistance; Resources; Reverse Transcriptase Polymerase Chain Reaction; Secondary to; Services; Silver; Social Distance; Solid; Source; South Carolina; Surface; Swab; Technology; Testing; Textiles; Thick; Thinness; Time; Topical application; Touch sensation; Viral; Viral Load result; Viscosity; Water; anti-viral efficacy; antimicrobial; antimicrobial drug; aqueous; bactericide; base; disease transmission; efficacy study; experience; experimental study; health care settings; human coronavirus; member; microbial; novel; novel coronavirus; pandemic disease; pathogen; phase 1 study; prevent; prisma; prototype; respiratory; transmission process; virucide

Project Summary A novel coronavirus (SARS-CoV-2) has become one of the greatest challenges of the modern world and has caused a pandemic with millions of infected people and hundreds of thousands of deaths around the globe. Transmissions occur through respiratory droplets, as well as through contaminated hands and environmental surfaces. Therefore, cleaning and disinfection of potentially contaminated surfaces becomes one of the most important measures in prevention of coronavirus transmission, along with social distancing and hand hygiene. Current CDC guidelines for preventing transmission of coronavirus specifically recommend cleaning and disinfection of frequently touched surfaces. The use of commercially available disinfectants significantly reduces microbial counts on environmental surfaces for only up to 4 hours after treatment. An alcohol-based disinfectant with a compound capable of forming a durable but removable biocidal film could combine the advantages of both approaches described above. It would combine high short-term efficacy due to the presence of alcohol and prevent microbial re-contamination due to the long-lasting efficacy of the film, while requiring less frequent applications. VRM Labs has recently developed such disinfectant, which contains 3 wt. % chitosan and 60 % wt. ethanol. Chitosan is obtained from crustacean shells and its antimicrobial and antiviral efficacy is well-established. However, it has not been used in surface disinfectants because of high viscosity of its aqueous solutions and long drying times required to form a continuous film. Our proposed formulation has low viscosity, outstanding film-forming properties and drying time similar to that for currently used disinfectants. In preliminary studies, door handles treated by this novel formulation prevented microbial growth for up to 48 hours after treatment. The goal of this Phase I study is to create an optimized prototype of chitosan-based disinfectant and to test its bactericidal and viricidal performance in a limited-scale study in a healthcare setting. In Aim I, we will characterize film formation properties, including film thickness, film durability, abrasion resistance, and film drying times for formulations with different chitosan and ethanol concentrations. We will also evaluate in vitro antimicrobial and antiviral efficacy of these chitosan-based coatings with the most common pathogens involved known to be a source of infection on environmental surfaces, and with a surrogate coronavirus to mimic antiviral action against COVID-19. In Aim II, we will use the optimized formulation developed in Aim I to coat several designated elevator buttons, door handles, and bed rails in a medical facility (Greenville Memorial Hospital in South Carolina). We will then collect environmental swabs from these coated surfaces on a regular basis and compare the microbial and viral counts to those obtained from similar surfaces treated according to the current hospital protocol. These experiments will help to determine the efficacy time window for the coating.

项目概要 新型冠状病毒（SARS-CoV-2）已成为现代世界面临的最大挑战之一， 在全球范围内造成了数百万人感染和数十万人死亡的大流行。 通过呼吸道飞沫以及受污染的手和环境传播 表面。因此，对潜在污染表面的清洁和消毒成为最重要的措施之一 预防冠状病毒传播的重要措施，以及保持社交距离和手部卫生。 目前疾病预防控制中心预防冠状病毒传播的指南特别建议清洁和 对经常接触的表面进行消毒。 使用市售消毒剂可显着减少环境表面的微生物数量 治疗后最多只能维持 4 小时。一种含酒精的消毒剂，其化合物能够形成 耐用但可移除的杀菌膜可以结合上述两种方法的优点。它会 结合由于酒精的存在而产生的高短期功效，并防止由于酒精的存在而导致的微生物再污染 薄膜的功效持久，同时需要较少的使用频率。 VRM Labs 最近开发了这种消毒剂，其中含有 3 wt.%。 % 壳聚糖和 60% 重量。乙醇。 壳聚糖是从甲壳类动物的壳中提取的，其抗菌和抗病毒功效已得到证实。 但由于其水溶液粘度高，且尚未用于表面消毒剂。 形成连续薄膜需要较长的干燥时间。我们提出的配方具有低粘度、优异的 成膜特性和干燥时间与目前使用的消毒剂相似。在初步研究中，门 经过这种新型配方处理的手柄在处理后可防止微生物生长长达 48 小时。 该第一阶段研究的目标是创建基于壳聚糖的消毒剂的优化原型并测试其 在医疗保健环境中进行的有限规模研究中的杀菌和杀病毒性能。在目标 I 中，我们将描述 成膜性能，包括膜厚度、膜耐久性、耐磨性和膜干燥时间 不同壳聚糖和乙醇浓度的制剂。我们还将评估体外抗菌和 这些基于壳聚糖的涂层对已知最常见病原体的抗病毒功效 环境表面的感染源，并用替代冠状病毒来模拟抗病毒作用 新冠肺炎。在Aim II中，我们将使用Aim I中开发的优化配方来涂覆多个指定的电梯 医疗机构（南卡罗来纳州格林维尔纪念医院）的按钮、门把手和床栏。我们 然后定期从这些涂层表面收集环境拭子并比较微生物 以及病毒计数与根据当前医院方案处理的类似表面获得的病毒计数。这些 实验将有助于确定涂层的功效时间窗口。

项目成果

Highly Adhesive Antimicrobial Coatings for External Fixation Devices.

用于外部固定装置的高粘性抗菌涂层。

• 发表时间: 2023-08-08
• 作者: Bredikhin, Mikhail;Sawant, Sushant;Gross, Christopher;Antonio, Erik L S;Borodinov, Nikolay;Luzinov, Igor;Vertegel, Alexey
• 通讯作者: Vertegel, Alexey