Divergent Functional and Metabolic Development of the Infant Microbiome

婴儿微生物组的不同功能和代谢发育

基本信息

批准号：
10652430
负责人：
Susan Veronica Lynch
金额：
$ 31.04万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-10 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10652430
关键词：
2 year old Allergic Arizona Asthma Bacteria Biological Assay Birth Border Community Border Crossings Cells Childhood Childhood Asthma Data Development Disparate Disparity Electrical Resistance Environment Environmental Exposure Epithelium Exhibits FOXP3 gene Feces Frequencies Genes Genetic House Dust Human IL4 gene IgE Immune Immunity In Vitro Infant Infant Development Inherited Intestines Life Location Meconium Medicine Metabolic Metabolic dysfunction Metagenomics Mexican Mexico Microbe Mothers Nature Neonatal Newborn Infant Outcome Pattern Population Preparation Prevalence Production Relative Risks Residencies Resolution Ribosomal RNA Risk Sampling Shotguns Source T-Lymphocyte Testing Vagina Water Supply Wheezing Woman atopy cohort drinking water experience fetal gut inflammation gut microbiome gut microbiota high risk high risk infant immune function immunological status immunoregulation in utero infancy infant gut microbiome maternal microbiome member metabolomics microbial microbial composition microbiome microbiome alteration microbiome components microbiome composition microbiome research microbiota mouse model neonate offspring peer pregnant prenatal stool sample transcriptome sequencing transmission process vaginal microbiota water sampling

项目摘要

BEAMS ABSTRACT: Project 2 Diminished early life environmental microbial exposures and perturbations to the infant gut microbiome are related to asthma outcomes in childhood. Mexican populations resident in Nogales, Mexico exhibit a four-fold decrease in childhood asthma rates compared with a population of comparable ancestry resident seventy miles away across the border in Tucson, Arizona. Our preliminary data has demonstrated that these cross-border populations experience significantly distinct microbial exposures in their environment (house dust and drinking water) and that the stool microbiota of 1-month old infants raised in these environments are differentially enriched for immunomodulatory bacterial genera. This suggests that microbial differentials in environmental and personal microbiomes relate to the cross-border disparity in asthma rates observed in this population. BEAMS proposes to comprehensively examine a population of Mexican mother-infant dyads resident in Nogales, Mexico or Tucson, Arizona and their environment in early life to determine how differences in environmental, maternal and infant microbial composition and function relate to asthma disparities in this cross-border population. Project 2 will examine at high-resolution the functional attributes of the microbiome of a subset of paired maternal prenatal (vaginal and stool) and infant gut (longitudinally collected stool) samples collected from 200 mother-infant dyads, as well as their paired house dust and water samples using shotgun metagenomics, RNA-Seq and metabolomics. The study will examine functional differentials in inherited microbes and gut microbiome development in cross-border communities that relate to T2 wheezing at age 2 years (a robust predictor of asthma in later childhood) and immune development. These efforts aim to identify environmental, maternal and infant microbiome attributes that promote protection against asthma within Mexican populations.

梁摘要：项目 2 生命早期环境微生物暴露减少以及对婴儿肠道微生物群的干扰与儿童时期哮喘的结局有关。居住在墨西哥诺加利斯的墨西哥人口的比例是原来的四倍与七十英里内具有可比血统的居民相比，儿童哮喘发病率下降越过边境到达亚利桑那州图森市。我们的初步数据表明，这些跨境人群在其环境中经历显着不同的微生物暴露（室内灰尘和饮水）水），并且在这些环境中长大的 1 个月大婴儿的粪便微生物群存在差异丰富对于免疫调节细菌属。这表明环境和个人中的微生物差异微生物组与该人群中观察到的哮喘发病率的跨境差异有关。 BEAMS 提出全面检查居住在墨西哥诺加利斯的墨西哥母婴二人群体，或亚利桑那州图森市及其生命早期的环境，以确定环境、母亲和母亲的差异如何婴儿微生物的组成和功能与这一跨境人群的哮喘差异有关。项目2 将以高分辨率检查配对母体产前子集的微生物组的功能属性从 200 名母婴二人中采集的（阴道和粪便）和婴儿肠道（纵向收集的粪便）样本，以及他们使用鸟枪宏基因组学、RNA-Seq 和配对的房屋灰尘和水样本代谢组学。该研究将检查遗传微生物和肠道微生物组的功能差异与 2 岁时 T2 喘息相关的跨境社区的发展（哮喘的有力预测因子）儿童后期）和免疫发育。这些努力旨在确定环境、孕产妇和婴儿促进墨西哥人群预防哮喘的微生物组属性。