Functional Analysis of TEM5 and TEM8

TEM5和TEM8的功能分析

• 批准号: 7733050
• 负责人: Bradley D St Croix
• 金额: $ 23万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7733050
• 关键词: Adult; Blood; Blood Vessels; Cell surface; Endothelial Cells; Gene Expression; Genes; Goals; Growth; Knockout Mice; Mus; Physiological; Role; Therapeutic; Tumor Angiogenesis; Tumor Cell Line; angiogenesis; gene function; interest; knockout gene; tumor; tumorigenesis

Tumors are dependent upon new blood vessel formation, or angiogenesis, for expansive growth. Our prior analysis of gene expression led to the identification of several genes upregulated in endothelial cells that line tumor blood vessels, called TEMs. Two of these, TEM5 and TEM8 encode products that are of particular interest because they reside on the cell surface and may therefore be directly accessible to blood-borne therapeutics. In an attempt to understand the functional role of these genes in angiogenesis, we have generated TEM5 and TEM8 knockout mice. By challenging the gene disrupted mice with tumors, we are evaluating the importance of these genes in tumor angiogenesis. These studies are also enabling us to better understand the normal physiological function of these genes particularly as it relates to angiogenesis.

肿瘤取决于新的血管形成或血管生成，以进行广泛的生长。我们先前对基因表达的分析导致鉴定在肿瘤血管（称为TEMS）的内皮细胞中上调的几个基因。其中两个，TEM5和TEM8编码特别感兴趣的产品，因为它们驻留在细胞表面，因此可以直接用于血液传播的疗法。为了了解这些基因在血管生成中的功能作用，我们已经产生了TEM5和TEM8敲除小鼠。通过挑战基因用肿瘤破坏小鼠，我们正在评估这些基因在肿瘤血管生成中的重要性。这些研究也使我们能够更好地理解这些基因的正常生理功能，尤其是与血管生成有关。