Clinical Orthopaedics

临床骨科

• 批准号: 7732844
• 负责人: ROCKY S TUAN
• 金额: $ 45.12万
• 依托单位: NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7732844
• 关键词: 3-Dimensional; Acute suppurative arthritis due to bacteria; Affect; Animal Model; Anoxia; Apoptosis; Area; Arthralgia; Bacterial Infections; Biological Models; Bone Growth; Cartilage; Cells; Clinical; Condition; Cultured Cells; Degenerative polyarthritis; Detection; Development; Diagnostic; Disease; Distraction Osteogenesis; Etiology; Event; Gene Expression; Glucosamine; Goals; Implant; In Vitro; Infection; Inflammatory; Interleukin-1 beta; Knowledge; Lead; Mechanics; Mediating; Messenger RNA; Modality; Modeling; Molecular; Musculoskeletal Diseases; Orthopedic Surgery procedures; Orthopedics; Oryctolagus cuniculus; Osteolysis; Pathogenesis; Physiological; Polymerase Chain Reaction; Procedures; Reverse Transcription; Ribosomal RNA; Role; Series; Simulate; Synovial Fluid; Technology; Testing; Therapeutic; Tissues; adult stem cell; articular cartilage; base; cell type; clinical application; cytokine; implant material; in vivo; mouse model; particle; postnatal; response; tool

1. Wear particle mediated osteolysis - We have clearly established that osteoprogenitor cells represent one of the target cell types dramatically affected by the presence of wear debris particles derived from metallic orthopaedic implant materials. Particle treated adult stem cells slow down in their proliferation, are induced to undergo apoptosis, and are inhibited in osteogenic differentiation. Our recent study focuses on the possible role of cytokines and anoxia in mediated these effects. 2. Molecular detection of orthopaedic infections - We have developed polymerase chain reaction (PCR)-based technologies for the detection of bacterial infection in orthopaedically relevant tissues, specifically septic arthritis and periprosthetic infection. By targeting RNA, both mRNA and rRNA, we are able to use quantitative reverse transcription-PCR (qRT-PCR) to detect as well as assess the viable bacterial load. A clinical series utilizing synovial fluid is being analyzed to test potential clinical application of the technology. 3. Supraphysiological impact mediated cartilage degeneration model - We have developed a reproducible, spring-loaded impactor based rabbit model to study impact-induced articular degeneration. We are focusing on the analysis of early cellular responses that lead to subsequent osteoarthritis-likd articular cartilage degeneration. 4. Animal model of distraction osteogenesis - A unique and effective procedure to induce postnatal bone growth, the mechanism of distraction osteogenesis is not well understood. We have developed and completed a study of a mouse model to analyze the gene expression events accomanying distraction osteogenesis. In addition, we are developing an in vitro mechanical activation model using culture cells in 2-D and 3-D conditions to simulate distraction osteogenesis. 5. Glucosamine as a chondroprotecive agent - The nutriceutical, glucosamine, has been widely used for joint pain and other related disease states. However, the mechanism of action remains elusive. We have investigated the efficacy of glucosamine in blocking the chondrodestructive activity of interleukin-1 beta. Our results showed that early or concomitant treatment with physiological concentrations of glucosamine effectively suppress the deleterious effects of the pro-inflammatory cytokine.

1。磨损颗粒介导的骨溶解 - 我们已经清楚地确定了骨源细胞代表了受到金属骨科植入物材料的磨损碎屑颗粒的显着影响的目标细胞类型之一。 颗粒处理的成年干细胞在增殖中降低，诱导凋亡，并在成骨分化中抑制。 我们最近的研究重点是细胞因子和缺氧在介导这些作用中的可能作用。 2。骨科感染的分子检测 - 我们开发了聚合酶链反应（PCR）基于基于骨质相关的组织中细菌感染的基于聚合酶链反应（PCR）的技术，具体是败血性关节炎和人体周感染。通过靶向RNA，mRNA和rRNA，我们能够使用定量逆转录PCR（QRT-PCR）来检测和评估可行的细菌载荷。 正在分析利用滑液的临床系列，以测试该技术的潜在临床应用。 3。超生理影响介导的软骨变性模型 - 我们开发了一种可再现的，弹簧撞击器的兔子模型，以研究影响撞击诱导的关节变性。 我们专注于对早期细胞反应的分析，这些反应导致随后的骨关节炎关节软骨变性。 4。分散注意力的动物模型 - 诱导产后骨骼生长的独特而有效的程序，分散注意力的机制尚不清楚。 我们已经开发了小鼠模型的研究并完成了一项研究，以分析基因表达事件的分心分散成骨。 此外，我们正在使用二-D和3-D条件下的培养细胞开发一种体外机械激活模型，以模拟分心的成骨。 5。葡萄糖胺作为软骨剂 - 营养素，葡萄糖胺，已被广泛用于关节疼痛和其他相关疾病状态。 但是，行动机制仍然难以捉摸。 我们已经研究了葡萄糖胺在阻断白介素-1β的软骨造成活性方面的功效。 我们的结果表明，早期或同时使用生理浓度的葡萄糖治疗有效地抑制了促炎性细胞因子的有害作用。