Flow Cytometry Laboratory Shared Resource

流式细胞术实验室共享资源

• 批准号: 10456689
• 负责人: Manish S Patankar
• 金额: $ 11.02万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-25 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10456689
• 关键词: Advisory Committees; Biological Assay; Biometry; Cancer Center; Cancer Center Support Grant; Cell Separation; Cells; Comprehensive Cancer Center; Computer Hardware; Computer software; Consult; Consultations; Custom; Cytometry; Data; Data Analyses; Data Set; Education; Educational workshop; Ensure; Etiology; Faculty; Feedback; Flow Cytometry; Fluorescence; Funding; Future; Generations; Image Cytometry; Informatics; Knowledge; Laboratories; Laboratory Personnel; Malignant Neoplasms; Mission; Monitor; NCI Center for Cancer Research; Population; Prevention; Procedures; Reagent; Reproducibility; Research; Research Support; Resource Sharing; Schedule; Services; Sorting - Cell Movement; Strategic Planning; Technology; Training; Universities; Vendor; Wisconsin; base; cancer therapy; complex data; cost; cost effective; data acquisition; design; experimental study; fluorophore; informatics shared resource; instrument; instrumentation; knowledge base; member; operation; programs; success

PROJECT SUMMARY / ABSTRACT The Flow Cytometry Laboratory (FLOW) was established by the UWCCC in 1988 to provide state-of-the-art instrumentation and expertise that enables our members to acquire and analyze complex data sets on single cells and cell populations and to isolate defined cell populations for further study. In the current CCSG funding cycle, FLOW has supported the research of 137 unique UWCCC members representing all 6 of our scientific programs. We have acquired new instrumentation, increased our capacity, established new services, and expanded our educational programs directed to UWCCC members, their trainees, and staff. We have engaged UWCCC leaders and our Scientific Advisory Committee to develop a strategic plan that will enable us to continue to excel in our mission and meet the rapidly evolving research needs of UWCCC members. Over the course of the next funding cycle we will achieve the following aims. Specific Aim 1 is to provide UWCCC members access to state-of-the-art instrumentation for single cell analytics and sorting. FLOW houses, operates, and maintains the broad assortment of instruments required for multi-parameter analytical cytometry, imaging cytometry, and cell sorting. FLOW has established Standard Operating Procedures (SOPs) that ensure all instruments are properly calibrated and operated in the manner required to yield high quality datasets that achieve accepted standards for experimental rigor and reproducibility. Specific Aim 2 is to provide expertise required for acquisition and analysis of multi-parameter flow cytometry data. Cell analytics and sorting technologies are advancing rapidly, and FLOW leaders monitor these advances and strive to ensure UWCCC members are able to fully exploit the newest technologies in their research. FLOW staff are experts in developing reagent panels that enable members to utilize our instruments to collect multi-parameter datasets with minimal spectral overlap. In addition, FLOW is developing a collaborative initiative with our Biostatistics and Cancer Informatics Shared Resources to ensure that the multi-parameter datasets generated on our instruments have appropriate power for robust statistical and informatics analyses. Specific Aim 3 is to provide expert consultation and education services that enable our users to take full advantage of our capabilities in single cell analytics and cell sorting. FLOW staff members consult with users to help them optimize assay design, data acquisition, data analysis, and data presentation. We provide guidance on instrument selection and operation. We inform users on incorporating appropriate technical controls into their experiments. FLOW offers regularly scheduled didactic courses and hands-on training to our users to advance their knowledge of flow cytometry and enhance their ability to perform high quality research at the lowest possible cost. FLOW organizes seminars, workshops, and vendor presentations that expand the knowledge base of our users. Support from the CCSG will enable us to continue to provide high level, cost-effective services to UWCCC members and thereby advance the UWCCC research mission.

项目概要/摘要 流式细胞术实验室 (FLOW) 由 UWCCC 于 1988 年建立，旨在提供最先进的检测技术 仪器和专业知识使我们的会员能够在单一设备上获取和分析复杂的数据集 细胞和细胞群，并分离确定的细胞群以供进一步研究。 在目前的 CCSG 资助下 周期中，FLOW 支持了代表我们所有 6 个科学领域的 137 名独特 UWCCC 成员的研究 程序。 我们购买了新仪器，提高了产能，建立了新服务，并 扩大了针对 UWCCC 会员、学员和员工的教育计划。我们已经订婚了 UWCCC 领导人和我们的科学咨询委员会制定战略计划，使我们能够 继续履行我们的使命，满足 UWCCC 成员快速变化的研究需求。超过 在下一个资助周期过程中，我们将实现以下目标。具体目标 1 是提供 UWCCC 成员可以使用最先进的单细胞分析和分选仪器。流动的房子， 操作并维护多参数分析细胞术所需的各种仪器， 成像细胞术和细胞分选。 FLOW 制定了标准操作程序 (SOP) 确保所有仪器均按照产生高质量所需的方式正确校准和操作 达到公认的实验严谨性和可重复性标准的数据集。具体目标 2 是 提供多参数流式细胞术数据采集和分析所需的专业知识。细胞分析 和分选技术正在迅速发展，FLOW 领导者密切关注这些进步并努力 确保 UWCCC 成员能够在其研究中充分利用最新技术。 FLOW 员工是 开发试剂组的专家使会员能够利用我们的仪器收集多参数 具有最小光谱重叠的数据集。此外，FLOW 正在与我们开发一项合作计划 生物统计学和癌症信息学共享资源，确保生成的多参数数据集 我们的仪器具有适当的能力进行稳健的统计和信息学分析。具体目标 3 是 提供专家咨询和教育服务，使我们的用户能够充分利用我们的 单细胞分析和细胞分选的能力。 FLOW 工作人员与用户协商以帮助他们 优化测定设计、数据采集、数据分析和数据呈现。我们提供指导 仪器的选择和操作。我们告知用户将适当的技术控制纳入他们的 实验。 FLOW 为我们的用户提供定期的教学课程和实践培训，以促进其进步 他们对流式细胞术的了解，并提高他们以最低成本进行高质量研究的能力 可能的成本。 FLOW 组织研讨会、讲习班和供应商演示，以扩展知识 我们的用户基础。 CCSG 的支持将使我们能够继续提供高水平、具有成本效益的服务 为 UWCCC 成员提供服务，从而推进 UWCCC 的研究使命。