RESPONSES OF INBRED MICE TO ECTROMELIA VIRUS

近交系小鼠对兔斑病毒的反应

• 批准号: 3421450
• 负责人: DAVID G BROWNSTEIN
• 金额: $ 20.07万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-01-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3421450
• 关键词: cellular immunity; communicable disease transmission; enzyme linked immunosorbent assay; epizootiology; genetic strain; inbreeding; laboratory mouse; male castration; pathology; serology /serodiagnosis; veterinary medicine; veterinary science; virus cytopathogenic effect; virus infection mechanism

Mousepox is a widely feared disease of laboratory mice that recently caused severe epizootics in the U.S.A. The proposed research will make comparative clinical, pathogenetic, and epizootiological observations in prototype inbred mice - BALB/c (susceptible) and C57BL/6 (resistant)-infected with 2 strains of the causative agent, ectromelia virus: a U.S. isolate known as the NIH-79 strain, and the well known Moscow strain. This comparison is essential to validate new approaches to the control and elimination of mousepox derived from studies of mice infected with the U.S. strain. The role of airborne transmission in the epizootiology of mousepox also will be assessed. Virological, pathological, and immunological markers in susceptible and resistant mice will be used to detect early events in infection of resistant mice. These experiments will use virus quantitation, immunohistochemical identification of lymphoid cell types, in in situ cellular responses and cytotoxic cell responses in vitro. Preliminary genetic studies will attempt to determine the genetic basis for resistance. Some of these studies anticipate the use of recombinant inbred strains for identification of genes governing resistance and susceptibility to mousepox. The results of this research will help to develop new approaches for detection, prevention and control of mousepox.

Mousepox是一种广泛担心的实验室小鼠疾病 最近在美国引起了严重的Epizootics。 研究将使比较临床，致病和 原型Inbrod小鼠原型的exootiologology观察结果-BALB/C （易感）和C57BL/6（抗性），并感染了2种菌株 病毒剂，Ectromelia病毒：一种称为美国的分离物 NIH-79菌株和著名的莫斯科菌株。 这 比较对于验证对控制的新方法至关重要 并消除源自小鼠研究的菌丝 感染了美国菌株。 空降传播的作用 在摩西司毒素的epizootiology中，还将评估。 病毒学，病理和免疫学标记 易感和抗性小鼠将用于检测早期事件 在抗性小鼠的感染中。 这些实验将使用病毒 定量，淋巴的免疫组织化学鉴定 细胞类型，原位细胞反应和细胞毒性细胞 体外反应。 初步的遗传研究将尝试 确定电阻的遗传基础。 其中一些 研究预期使用重组近交菌株 鉴定有关抗药性和易感性的基因 穆斯科克斯。 这项研究的结果将有助于开发新的 用于检测，预防和控制菌的方法。