SEROLOGIC DETERMINATION OF LEPROSY IN CHIMPANZEES

黑猩猩麻风病的血清学测定

• 批准号: 3421538
• 负责人: BOBBY J GORMUS
• 金额: $ 10.35万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-04 至 1993-09-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3421538
• 关键词: Mycobacterium leprae; Pan; animal colony; cell wall; communicable disease control; communicable disease diagnosis; enzyme linked immunosorbent assay; epizootiology; immunoglobulin G; immunoglobulin M; information systems; leprosy; longitudinal animal study; serology /serodiagnosis; surface antigens; veterinary medicine; Mycobacterium leprae; Pan; animal colony; cell wall; communicable disease control; communicable disease diagnosis; enzyme linked immunosorbent assay; epizootiology; immunoglobulin G; immunoglobulin M; information systems; leprosy; longitudinal animal study; serology /serodiagnosis; surface antigens; veterinary medicine

Longitudinally-obtained serum samples from chimpanzees in the Dedicated Breeding Program (DBP) will be studied by ELISA to determine the titers of IgG and IgM antibodies to Mycobacterium leprae-specific cell wall phenolic glycolipid-I (PGL-I) antigen and the mycobacterial common cell wall antigen lipoarabinomannan (LAM). The antibody profiles will detect chimpanzees that have been exposed to leprosy, a disease only recently shown to exist among the DBP chimps, and will identify individuals that are likely to have sub-clinical leprosy. These animals will be isolated and studied in more detail. To confirm the presence of M. leprae, sera from chimpanzees testing positive for subclinical leprosy will be studied further by a dot ELISA to identify specific PGL-I antigen and by assaying the sera by ELISA for a newly identified M. leprae-specific 35 Kd cell wall protein antigen. The origin of leprosy in the DBP and epidemiologic relationship between M. leprae exposed/infected animals will be explored using the International Species Information System (ISIS) databank that records all inter- Institutional chimpanzee transfers. Information obtained from the participating Institutions regarding caging arrangements and physical contacts between identified exposed/infected chimps and other breeders will be posure/infection, and epidemiological relationships will be sought by comparing the two sets of longitudinal data. Since leprosy has not been previously recognized among primates other than man (and now, perhaps, sooty mangabey monkeys), the ISIS epidemiologic study will be important in tracing the origin of the disease in the DBP. It is important to determine the prevalence of M. Leprae infection among the breeding chimpanzees since preliminary studies suggest that this species may be more susceptible to naturally-acquired leprosy than might be supposed. Further spread of leprosy among the DBP animals can be halted by identification, isolation and chemotherapy of the exposed individuals.

来自黑猩猩的纵向血清样品 ELISA将研究育种计划（DBP），以确定 IgG和IgM抗体对麻风病的分枝杆菌特异性细胞壁酚类 糖脂蛋白I（PGL-I）抗原和分枝杆菌公共细胞壁抗原 脂肪动物群（LAM）。 抗体曲线将检测黑猩猩 暴露于麻风病，这种疾病直到最近才存在 在DBP的黑猩猩中，将确定可能拥有的个人 临界麻风病。 这些动物将被隔离和研究 细节。 为了确认Leprae M. Leprae的存在，来自黑猩猩测试的血清 亚临床麻风病的阳性将通过DOT ELISA进一步研究 识别特定的PGL-I抗原并通过ELISA测定A的血清 新鉴定的麻风麻部特异性35 kD细胞壁蛋白抗原。 麻风病的起源在DBP和M.之间的流行病学关系中 麻风病暴露/感染的动物将使用国际 物种信息系统（ISIS）数据库，记录所有相互关系 机构黑猩猩转移。 从 参与机构有关笼子安排和身体的机构 已识别的暴露/感染黑猩猩与其他育种者之间的接触将 出现 - 感染，流行病学关系将由 比较两组纵向数据。 由于麻风病以前尚未在以外的灵长类动物中得到认可 人（现在，也许是烟熏芒果猴子），ISIS流行病学 研究对于追踪DBP疾病的起源至关重要。 确定麻风开杆菌感染的患病率很重要 自初步研究以来的繁殖黑猩猩表明这一点 物种可能比可能更容易受到自然性的麻风病的影响 假设。 麻风病在DBP动物中的进一步传播可以停止 暴露的个体的鉴定，隔离和化学疗法。