EXPERIMENTAL LEPROSY & VACCINE STUDIES IN MONKEYS: MYCOBACTERIA

实验性麻风病

• 批准号: 6277407
• 负责人: BOBBY J GORMUS
• 金额: $ 8.01万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6277407
• 关键词: Mammalia; Primates; animal tissue; bacteria; immunology; infection; inflammation; model design /development; nervous system; skin; vaccines

We analyzed and evaluated longitudinal immunologic data gathered over a 3-4 year period post vaccination (PV) (with live BCG or BCG + heat-killed Mycobacterium leprae, HKML), post-boosting (PB) and post-challenge (PC) with live ML. Rhesus monkeys (RM) [paucibacillary (PB)-prone, as a species] were significantly protected clinically by BCG or BCG + HKML; sooty mangabey monkeys (SMM) [multibacillary (MB)-prone, as a species] were partially protected by BCG alone, but were rendered clinically more susceptible by BCG + HKML. Consistent parallels were seen between clinical results and blastogenic responses of blood mononuclear cells to ML antigens and to tuberculin, serum anti-phenolic glycolipid-I (PGL-I) antibody isotype (IgG & IgM) responses, serum PGL-I antigen levels, blood lymphocyte subsets carrying CD4, CD8, CD16, CD20, CD4CD29 and CD4CD45 phenotypic markers and lepromin skin test results. There are multiple complex, interrelated dose-dependent relationships in immunologic responses to ML antigens that determine leprosy susceptibility/resistance. The mechanisms may involve subtle differences between RM and SMM in cytokine production and/or responses, which model responses in individual humans with PB vs MB predispositions. Studies are in progress to determine comparative cytokine profiles by PCR in longitudinally-obtained lesion biopsies from animals in this study to help

我们分析和评估了收集的纵向免疫数据 疫苗接种后3 - 4年（PV）（现场BCG或BCG + 热杀性分枝杆菌麻风病，hkml），增强后（PB）和 挑战后（PC），带有实时ML。 恒河猴（RM） （Pb） - 统治，作为一个物种]在临床上受到了显着保护 BCG或BCG + HKML;烟草芒果猴（SMM） （MB） - 统治，作为一个物种]仅由BCG部分保护，但 BCG + hkml在临床上更容易敏感。 持续的 在临床结果和胚泡反应之间看到了相似之处 血液单核细胞向ML抗原和结核蛋白，血清 抗苯酚甘油果脂I（PGL-I）抗体同种型（IgG和IgM） 反应，血清PGL-1抗原水平，血液淋巴细胞子集 携带CD4，CD8，CD16，CD20，CD4CD29和CD4CD45表型标记 和Lepromin皮肤测试结果。 有多个复杂， 免疫反应中相互关联的剂量依赖性关系 确定麻风病敏感/耐药性的ML抗原。 这 机制可能涉及RM和SMM之间的细微差异 细胞因子的产生和/或反应，这些反应在 具有PB与MB易感性的个别人类。 研究正在进行中 进展以确定PCR中比较细胞因子谱 在这项研究 帮助