SPINOHYPOTHALAMIC AND SPINOTELENCEPHALIC TRACTS

脊髓下丘脑和脊髓端脑束

• 批准号: 3411487
• 负责人: GLENN JOSEPH GIESLER
• 金额: $ 9.6万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-06-01 至 1991-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3411487
• 关键词: Macaca; axon; brain mapping; brain septal area; cats; central neural pathway /tract; hypothalamus; laboratory rat; neuroanatomy; neurons; nucleus accumbens; prosencephalon; spinal cord; telencephalon

Although hypothalamic neurons can be driven powerfully by nociceptive and other forms of somatosensory stimulation, the spinal input to this region has been generally believed to be multisynaptic. However, we have recently found that a large number of spinal cord neurons project directly to the hypothalamus and telencephalon in rats. Injections of any of three different retrograade tracers that were restricted to the hpothalamus labeled many neurons in the spinal cord. Labeled neurons were found primarily within the marginal zone, the lateral reticulated area (lamina V), the gray matter surrounding the central canal and the lateral spinal nucleus. Based on the results of such injections we have determined that there are more than 5000 neurons throughout the length of the spinal cord that project to the hypothalamus. Injections of the anterograde tracer PHAL into the spinal cord labeled fibers and varicosities in a number of hypothalamic nuclei and also within several telencephalic areas including the basal forebrain, septal nuclei and nucleus accumbens. Injections of retrograde tracers that were restricted to each of these telencephalic areas also labeled spinal cord neurons. We have physiologically examined 10 neurons thatat were antidromically activated wih low intesity current from the hypothalamus. All 10 responded to innocuous mechanical stimuli but responded more vigorously to noxious mechanical and thermal stimulation. In the proposed studies we will 1) continue our physiological studies of spinal cord nuerons that are antidromically activated from the hypothalamus, 2) physiologically examine spinal cord neurons that project to telencephalic nuclei, 3) determine the course of the axons within the spinal cord as they ascend to the hypothalamus and telencephalon, 4) use retrograde labeling techniques to determine if similar projections exist in cats and 5) determine if similar projections exist in monkeys using retrograde tracers. If such projections are found in monkeys, we will determine the distribution of their terminations within the hypothalamus and telencephalon and their course within the spinal cord. In addition, the response properties of their cells or orgin will be determined to a variety of innocuous and noxious cutaneous stimuli. These studies will provide important data on the spinal cord neurons that project directly to areas that are involved in autonomic, neuroendocrine and affective responses to nociceptive and other forms of somatosensory stimulation.

尽管下丘脑神经元可以被强有力地驱动 伤害性和其他形式的体感刺激， 人们普遍认为该区域的脊髓输入是 多突触。 然而，我们最近发现，大量的 脊髓神经元的数量直接投射到 大鼠的下丘脑和端脑。 注射任何 三种不同的逆行示踪剂仅限于 丘脑标记了脊髓中的许多神经元。 贴标签 神经元主要发现于边缘区， 外侧网状区（第五层），周围的灰质 中央管和外侧脊髓核。 基于 此类注射的结果我们确定有更多 整个脊髓有超过 5000 个神经元 投射到下丘脑。 顺行示踪剂注射 PHAL 进入脊髓，标记纤维和静脉曲张 下丘脑核团的数量，也在几个范围内 端脑区域，包括基底前脑、间隔核 和伏隔核。 注射逆行示踪剂 仅限于每个也标记为端脑的区域 脊髓神经元。 我们对 10 个神经元进行了生理检查 用低强度电流逆向激活 来自下丘脑。 所有 10 人均回复无害 机械刺激但对有害物质反应更强烈 机械和热刺激。 在拟议的研究中，我们 将1）继续我们对脊髓神经元的生理学研究 从下丘脑逆向激活，2) 生理学检查投射到的脊髓神经元 端脑核，3）决定内部轴突的走向 当脊髓上升到下丘脑时 端脑，4) 使用逆行标记技术来确定 猫中是否存在相似的投影，5）确定是否相似 使用逆行示踪剂的猴子中存在预测。 如果这样的话 在猴子身上发现了投射，我们将确定 它们的末端在下丘脑内的分布和 端脑及其在脊髓内的走向。 此外， 其细胞或来源的反应特性将被确定 各种无害和有害的皮肤刺激。 这些 研究将提供有关脊髓神经元的重要数据 直接投射到涉及自主的领域， 对伤害性和其他感受的神经内分泌和情感反应 体感刺激的形式。