SPINAL ORGANIZATION OF FUNCTION

脊柱的功能组织

• 批准号: 3411842
• 负责人: KEVIN E MCKENNA
• 金额: $ 10.64万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1991-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3411842
• 关键词: adrenergic agents; afferent nerve; antiadrenergic agents; chemoreceptors; electromyography; electrophysiology; histology; immunocytochemistry; male reproductive system; neural information processing; neurophysiology; neurotoxins; reflex; semen; sensory mechanism; sensory thresholds; serotonin; spinal reflex; stimulus /response; substance P; urethra

This proposal is a focused and rigorous test of a single hypothesis concerning male sexual function. Preliminary experiments with a novel preparation developed in this laboratory demonstrated that intraurethral stimuli elicited a fully developed ejaculatory-like response (the coitus reflex) in male rats. These findings have prompted a unifying hypothesis which proposes that ejaculation is induced by mechanical and chemical stimulation of the urethra by seminal fluids. The present proposal represents a definitive test of this theory using a multidisciplinary approach. The sensory responses elicited by seminal fluids and chemically inert fluids will be compared in quantitative electrophysiological experiments. The threshold (urethral pressure and flow) for evoking the coitus reflex will be measured to determine if seminal fluids are more stimulatory than chemically inert fluids, a strong indication of chemosensory mechanisms. Multi- and single-unit studies will examine the afferent response to these intraurethral stimuli. The presence of serotonergic paracrine cells have been demonstrated in the urethra sensory processes. This suggestion will be tested by immunohistochemical mapping studies, specific neurotoxic lesions with functional assessment, and pharmacological studies using specific serotonergic agonists and antagonists. Peptidergic afferents have been localized in the urogenital system and physiological studies suggest a role for peptidergic mechanisms in mediating some pelvic functions. The role of peptidergic afferents in the coitus reflux will be examined by immunohistochemical mapping, specific neurotoxic lesions with functional testing, release studies and pharmacological studies using tackykinin agonists.

该提案是对单一假设的集中且严格的测试 关于男性性功能。 初步实验 该实验室开发的新制剂表明 尿道内刺激引起完全发育的射精样 雄性大鼠的反应（性交反射）。 这些发现有 提出了一个统一的假设，即射精是 由尿道的机械和化学刺激引起 精液。 目前的提案代表了一次最终的测试 使用多学科方法研究该理论。 精液和化学物质引起的感觉反应 惰性液体将在定量电生理学方面进行比较 实验。 阈值（尿道压力和流量） 将测量唤起性交反射以确定是否精液 液体比化学惰性液体更具刺激性， 化学感应机制的指示。 多单元和单单元 研究将检查对这些尿道内的传入反应 刺激。 已证实存在血清素能旁分泌细胞 在尿道感觉过程中。 该建议将被测试 通过免疫组织化学图谱研究，特定的神经毒性病变 进行功能评估和药理学研究 特异性血清素激动剂和拮抗剂。 肽能传入已定位于泌尿生殖系统 生理学研究表明肽能机制的作用 调节一些骨盆功能。 肽能的作用 性交反流中的传入将通过以下方式检查 免疫组织化学图谱，特定神经毒性病变 功能测试、释放研究和药理学研究 使用粘激肽激动剂。