AGING LHRH SYSTEM/EM IMMUNOCYTOCHEMICAL STUDIES

老化 LHRH 系统/EM 免疫细胞化学研究

• 批准号: 3115972
• 负责人: JOAN W WITKIN
• 金额: $ 14.94万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-12-01 至 1994-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3115972
• 关键词: adrenocorticotropic hormone; aging; animal old age; antibody; axon; biological models; central nervous system; corticotropin releasing factor; cytochrome oxidase; dendrites; electron microscopy; endorphins; estrogen receptors; gamma aminobutyrate; genetic transcription; glia; histochemistry /cytochemistry; hypothalamus; immunochemistry; in situ hybridization; laboratory rat; limbic system; male; mature animal; median eminence; messenger RNA; neural plasticity; neuroanatomy; neuroendocrine system; neurons; neuropeptide Y; neuropeptides; neurotransmitters; radionuclide double label; sex hormones; synapses; synaptic vesicles; tyrosine 3 monooxygenase

This research is directed at understanding aging changes in the anatomy and synaptology of the luteinizing hormone-releasing hormone (LHRH) system. The animal model is the rat as this is the species on which most aging study has been done. The specific aims of the proposed studies are to investigate the ultrastructure of LHRH neurons and their dendrites in the preoptic area (POA) and their axons and terminal fields in the medial basal hypothalamus (MBH) and median eminence (ME) in the male rat, using adult (2-4 mos), middle aged (10-12 mos) and old (22-24 mos) animals. Age changes will be observed in the relationship of these neurons and their processes to their affective milieu. Four modulators of LHRH release which are known to be affected by aging have been chosen for these studies: norepinephrine, neurotensin, beta-endorphin and serotonin. Morphometric analyses will be made of immunocytochemically identified elements including perikaryal components (nucleus, nucleoli, rough endoplasmic reticulum, Golgi apparatus), dendrites, axon terminals, and pre- and post-synaptic characteristics. The proposed electron microscopic, immunocytochemical studies fall into three categories: 1. Aging changes in the ultrastructure and synaptology of LHRH neurons in the POA. a. Morphometric analysis and synaptology of LHRH neurons b. Analysis of noradrenergic input to LHRH neurons c. Study of interrelation between neurotensin and LHRH neurons 2. Aging changes in ultrastructure and input to LHRH neurons in MBH. a. Morphometric study of synaptology of LHRH processes and their relationship to each other and their affective milieu b. Analysis of LHRH processes and beta-endorphin neurons c. Analysis of serotoninergic input to LHRH processes 3. Aging changes in the ultrastructure of LHRH axons and their proximity to the fenestrated capillaries of the median eminence.

这项研究旨在了解解剖学的衰老变化和 黄体激素释放激素（LHRH）系统的突触学。 动物模型是大鼠，因为这是大多数老化的物种 研究已经完成。 拟议研究的具体目的是 研究LHRH神经元及其树突的超微结构 前式区域（POA）及其轴突和末端场 雄性大鼠的下丘脑（MBH）和中位数（ME），使用成人 （2-4 MOS），中年（10-12 MOS）和旧（22-24个MOS）动物。 年龄 这些神经元及其之间的关系将观察到变化 处理他们的情感环境。 LHRH发行的四个调制器 已知已被衰老的影响已被选中： 去甲肾上腺素，神经素，β-内啡肽和5-羟色胺。 形态计量学 分析将对免疫细胞化学鉴定的元素进行分析 牙周成分（核，核仁，粗糙的内质网， 高尔基体），树突，轴突末端以及突触前后 特征。 提出的电子显微镜，免疫细胞化学 研究分为三类： 1。在LHRH神经元的超微结构和突触学的衰老变化 POA。 一个。 LHRH神经元的形态分析和突触学 b。分析对LHRH神经元的去甲肾上腺素能输入 c。神经素和LHRH神经元之间的相互关系研究 2。超微结构的衰老变化和MBH中LHRH神经元输入。 一个。 LHRH过程突触术及其它们的形态计量学研究 彼此的关系及其情感环境 b。 LHRH过程和β-内啡肽神经元的分析 c。分析5-羟色胺能输入到LHRH过程 3. LHRH轴突超微结构的衰老变化及其邻近性 中位数毛细血管的毛细血管。