NUTRITIONAL REQUIREMENTS FOR IMMUNITY IN THE AGED

老年人免疫力的营养需求

• 批准号: 3121146
• 负责人: RICHARD A WINCHURCH
• 金额: $ 10.02万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3121146
• 关键词: B lymphocyte; animal old age; antibody formation; bone marrow; cell population study; cyclic AMP; dietary trace element; immunity; immunopharmacology; interleukin 1; interleukin 6; laboratory mouse; lymphocyte proliferation; metallothionein; northern blottings; nutrient requirement; nutrition disorder chemotherapy; nutrition of aging; nutrition related tag; protein biosynthesis; protein kinase; tissue /cell culture; tissue /cell preparation; zinc

The aging process is characterized by a progressive decline in the host's immune response profile. Moreover, optimal functioning of the immune system depends upon adequate levels of dietary zinc. In previous studies we have shown that supplemental zinc restores a large measure of immunocompetence to aging mice. In this proposal we endeavor to study possible mechanisms by which zinc activates antibody responses in immunodepressed, aged mice. Recent reports in the literature as well as preliminary data presented in this proposal suggest that interleukin 1 along with supplemental zinc constitute a minimum signal for the production of specific antibody by murine B cells. Further data suggest that the pathway by which B cells are activated involves cyclic AMP-dependent events. Using in vitro techniques we will culture purified populations of B lymphocytes from 24 month old mice. These cells will be activated with antigen along with interleukin 1 and will be supplemented with immunoenhancing, pharmacologic doses of zinc. Antibody responses will be monitored as will possible pathways of activation. Thus, we will examine the effects of I1-1 and zinc on cyclic AMP-dependent events, on interleukin 6 synthesis and on the induction of metal metallothionein mRNA synthesis.

衰老过程的特征是主机的逐步下降 免疫反应曲线。 此外，免疫的最佳功能 系统取决于足够的饮食锌。 在先前的研究中 我们已经表明，补充锌恢复了大量的 对衰老小鼠的免疫能力。 在这项建议中，我们努力学习 锌激活抗体反应的可能机制 免疫抑郁，老年小鼠。 文献的最新报告以及 本提案中提出的初步数据表明白介素1 以及补充锌构成生产的最低信号 鼠B细胞的特异性抗体。 进一步的数据表明 激活B细胞的途径涉及环状AMP依赖性 事件。 使用体外技术，我们将培养纯化的种群 来自24个月大的小鼠的B淋巴细胞。 这些单元将被激活 抗原与白介素1一起补充 免疫促，锌的药理学剂量。 抗体反应将是 按照可能的激活途径进行监测。 因此，我们将检查 I1-1和锌对循环AMP依赖性事件的影响，对白介素 6合成和金属金属蛋白mRNA合成的诱导。