NEURAL INFLUENCE ON AGING OF RECEPTOR CELLS

神经对受体细胞老化的影响

• 批准号: 3117628
• 负责人: STEPHEN D. ROPER
• 金额: $ 13.78万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-01-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3117628
• 关键词: Urodela; aging; autoradiography; cell age; cell growth regulation; chemoreceptors; denervation; electron microscopy; epithelium; familial dysautonomia; histochemistry /cytochemistry; mathematical model; microelectrodes; neurons; neurophysiology; radiotracer; scanning electron microscopy; single cell analysis; synapses; taste; taste buds; taste threshold; tongue

The vertebrate taste bud is a good model for studying cellular aging in the nervous system. Taste cells possess many characteristics shared by neurons, such as excitability, chemosensitivity, and synapses (Roper, 1983a; Kinnamon, Taylor, Delay and Roper, 1985; Kinnamon and Roper, 1986). Taste cells have the added advantage that their lifespan is short (only a few days) compared to neurons, and thus age-related changes can be measured with reliability and confidence: complicating factors such as metabolic or hormonal differences between young and old animals are avoided. Furthermore, detailed analyses of age- related changes in the biophysical properties of taste cells and in their membrane chemosensitivity can be conducted on taste cells. Taste cells from the mudpuppy (Necturus maculosus) will be impaled with microelectrodes in situ, using a new isolated preparation developed in this laboratory (Roper, 1983). In depth analyses of the membrane conductances, particularly potassium conductance, will shed light on how ionic channels change over the lifespan of an excitable cell. Chemical stimuli will be applied focally onto the apical surface of taste cells to sutdy what age- related changes in chemosensitivity occur. Cells will be impaled with dye-filled micropipettes to identify the cells in subsequent light and electron micrographs in order to correlate the functional properites of the cell with its age: recent observations have indicated that taste cell aging can be determined by ultrastructural features (Kinnamon, et al. 1985; Delay, Kinnamon and Roper, 1986). These findings will provide information on cellular aging in excitable tissues and will also lead to a better understanding of taste transduction mechanisms.

脊椎动物味蕾是研究细胞的好模型 神经系统衰老。 味道细胞拥有许多 神经元共有的特征，例如兴奋性， 化学敏感性和突触（Roper，1983a; Kinnamon，Taylor， Delay and Roper，1985年； Kinnamon and Roper，1986）。 味道细胞 还有一个额外的优势，即他们的寿命很短（只有少数 几天）与神经元相比，因此与年龄相关的变化可以是 以可靠性和信心来衡量：复杂因素 例如年轻人和老年人之间的代谢或激素差异 避免动物。 此外，对年龄的详细分析 味道细胞和在 它们的膜化学敏感性可以在味觉细胞上进行。 来自泥浆的味道细胞将 使用新的隔离 在该实验室中开发的准备（Roper，1983）。 深度 膜电导的分析，尤其是钾 电导，将阐明离子通道的变化 一个兴奋的单元的寿命。 化学刺激将被应用 聚焦在味觉细胞的顶端表面，以达到什么年龄 - 化学敏感性的相关变化发生。 细胞将被刺穿 用染料填充的微夹来识别随后的细胞 光和电子显微照片是为了使功能相关 及其年龄的细胞的适当人：最近的观察结果 表明味觉细胞衰老可以通过 超微结构特征（Kinnamon等，1985；延迟，Kinnamon 和Roper，1986）。 这些发现将提供有关细胞衰老的信息 可兴奋的组织，也将使人们更好地理解 口味转导机制。

项目成果

Passive and active membrane properties of mudpuppy taste receptor cells.

泥巴犬味觉受体细胞的被动和主动膜特性。

• DOI: 10.1113/jphysiol.1987.sp016431
• 发表时间: 1987
• 期刊: The Journal of physiology
• 作者: Kinnamon,SC;Roper,SD
• 通讯作者: Roper,SD

Membrane properties of two types of basal cells in Necturus taste buds.

Necturus 味蕾中两种基底细胞的膜特性。

• DOI: 10.1523/jneurosci.14-10-06132.1994
• 发表时间: 1994
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Delay,RJ;Mackay-Sim,A;Roper,SD
• 通讯作者: Roper,SD

Intercellular signaling in Necturus taste buds: chemical excitation of receptor cells elicits responses in basal cells.

Necturus 味蕾中的细胞间信号传导：受体细胞的化学激发引起基底细胞的反应。

• DOI: 10.1152/jn.1992.67.5.1316
• 发表时间: 1992
• 期刊: Journal of neurophysiology
• 影响因子: 2.5
• 作者: Ewald,DA;Roper,SD
• 通讯作者: Roper,SD

Dye-coupling in taste buds in the mudpuppy, Necturus maculosus.

泥巴犬 Necturus maculosus 味蕾中的染料耦合。

• DOI: 10.1523/jneurosci.07-11-03561.1987
• 发表时间: 1987
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: Yang,J;Roper,SD
• 通讯作者: Roper,SD