Concurrent multiphoton microscopy and magnetic resonance imaging (COMPMRI)

并行多光子显微镜和磁共振成像 (COMPMRI)

• 批准号: 9360610
• 负责人: Yi Wang
• 金额: $ 18.74万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-30 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9360610
• 关键词: Area; BRAIN initiative; Behavior; Biomedical Research; Brain; Brain region; Brain scan; Budgets; Cells; Craniotomy; Development; Environment; Epilepsy; Event; Fluorescence; Functional Magnetic Resonance Imaging; Genetic; Genetic Engineering; Head; Histology; Human; Image; Individual; Knowledge; Lasers; Link; Magnetic Resonance Imaging; Measures; Methodological Studies; Microscopy; Molecular; Mus; Myelin; Nervous system structure; Neurons; Optics; Pattern; Penetration; Photons; Preparation; RF coil; Research; Resolution; Seizures; Signal Transduction; Testing; Therapeutic; Thinness; Variant; base; biological research; cellular imaging; cranium; experience; high resolution imaging; imaging study; imaging system; multiphoton imaging; neural circuit; neuronal circuitry; programs; relating to nervous system; response; tool; two-photon

We propose a concurrent multiphoton magnetic resonance imaging (COMPMRI) system for mouse imaging, in response to RFA-EY-16-001, BRAIN Initiative: New Concepts and Early-Stage Research for Large-Scale Recording and Modulation in the Nervous System (R21). We synergistically combine multiphoton microscopy (MPM), which offers cellular imaging resolution with penetration depth, and Magnetic resonance imaging (MRI), which is routinely used to capture large-scale neural activities across many regions of the brain. To understand molecular cellular origin of the large-scale neural activity, we need simultaneous imaging both at cellular resolution and over the whole brain, which is concurrent MPM and MRI as in our proposed COMPMRI. Furthermore, we will leverage our latest breakthrough in MPM using 3-photon excitation with long wavelength that enables through-skull cellular resolution imaging, making it possible to perform MPM at multiple cortical regions. Our proposed COMPMRI will take advantage of this 3-photon microscopy and the large bore size of a 3T human MRI system to scan the brain of a live mouse. Our proposed COMPMRI system will allow simultaneous study of neural activities localized in a region and distributed over the whole brain.

我们提出了一种用于小鼠成像的并发多光子磁共振成像（COMPMRI）系统， 对 RFA-EY-16-001 的回应，BRAIN Initiative：大规模的新概念和早期研究 神经系统的记录和调制（R21）。我们协同结合多光子显微镜 (MPM)，提供具有穿透深度的细胞成像分辨率和磁共振成像 (MRI)，通常用于捕获大脑许多区域的大规模神经活动。到 为了了解大规模神经活动的分子细胞起源，我们需要同时成像 细胞分辨率和整个大脑，这是并行的 MPM 和 MRI，如我们提出的 COMPMRI 中一样。 此外，我们将利用长波长 3 光子激发在 MPM 方面的最新突破 能够实现贯穿颅骨的细胞分辨率成像，从而可以在多个皮质上执行 MPM 地区。我们提出的 COMPMRI 将利用这种 3 光子显微镜和大孔径的 3T 人体 MRI 系统扫描活体小鼠的大脑。我们提出的 COMPMRI 系统将允许 同时研究局部区域和整个大脑分布的神经活动。