Function of RecQ helicases in genome stability

RecQ 解旋酶在基因组稳定性中的功能

• 批准号: 10913133
• 负责人: Robert Brosh
• 金额: $ 5.7万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10913133
• 关键词: Aging; Biochemical; Biochemical Process; Cancerous; Cells; Characteristics; Code; DNA; DNA Damage; DNA Double Strand Break; DNA Repair; DNA Structure; Disease; Double Strand Break Repair; Enzymes; Genes; Genetic; Genome; Genome Stability; Genomic Instability; Human; Life; Light; Malignant Neoplasms; Mendelian disorder; Metabolic Pathway; Molecular; Mutate; Mutation; Nonhomologous DNA End Joining; Pathway interactions; Patients; Play; Premature aging syndrome; Process; Proteins; RECQL gene; RECQL4 gene; RECQL5 gene; Research; Role; Syndrome; Time; age related; helicase; homologous recombination; member; prevent; protein function

DNA damage accumulates during life and is thought to contribute to aging and genomic instability. Therefore, defining those proteins and pathways that maintain genomic stability is critical in preventing aging and age-related degeneration. This project aims to understand what roles human RecQ proteins play in DNA repair and genome stability. RecQ proteins play fundamental roles in several DNA metabolic pathways including DNA double-strand break repair (DSBR), including homologous recombination (HR), non-homologous end joining (NHEJ), and replication. Three of the five human RecQ helicases, WRN, BLM, and RECQL4, are associated with monogenic diseases. Understanding the biochemical processes that these protein function in and their shared or unique roles in DNA repair processes continues to be a focus of this research.

DNA损伤在生命中积累，被认为会导致衰老和基因组不稳定性。因此，定义维持基因组稳定性的蛋白质和途径对于预防衰老和与年龄相关的变性至关重要。该项目旨在了解人类RECQ蛋白在DNA修复和基因组稳定性中的作用。 RECQ蛋白在几种DNA代谢途径（包括DNA双链断裂修复（DSBR）（包括同源重组）（HR），非同源末端连接（NHEJ）和复制等几种DNA代谢途径中起基本作用。五种人RECQ解旋酶中的三个，即WRN，BLM和RECQL4，与单基因疾病有关。了解这些蛋白质功能及其在DNA修复过程中共享或独特作用的生化过程仍然是这项研究的重点。

项目成果

Skin Abnormalities in Disorders with DNA Repair Defects, Premature Aging, and Mitochondrial Dysfunction.

• DOI: 10.1016/j.jid.2020.10.019
• 发表时间: 2021-04
• 期刊: The Journal of investigative dermatology
• 作者: Hussain M;Krishnamurthy S;Patel J;Kim E;Baptiste BA;Croteau DL;Bohr VA
• 通讯作者: Bohr VA

DNA repair: front and center and not going away!

DNA 修复：前沿和中心且不会消失！

• DOI: 10.1007/978-1-61779-998-3_1
• 发表时间: 2012
• 期刊: Methods in molecular biology (Clifton, N.J.)
• 作者: Bohr,VilhelmA

A research agenda for aging in China in the 21st century.

• DOI: 10.1016/j.arr.2015.08.003
• 发表时间: 2015-11
• 期刊: AGEING RESEARCH REVIEWS
• 影响因子: 13.1
• 作者: Fang, Evandro Fei;Scheibye-Knudsen, Morten;Jahn, Heiko J.;Li, Juan;Ling, Li;Guo, Hongwei;Zhu, Xinqiang;Preedy, Victor;Lu, Huiming;Bohr, Vilhelm A.;Chan, Wai Yee;Liu, Yuanli;Ng, Tzi Bun
• 通讯作者: Ng, Tzi Bun

RECQL4 helicase has oncogenic potential in sporadic breast cancers.

• DOI: 10.1002/path.4681
• 发表时间: 2016-03
• 期刊: The Journal of pathology
• 作者: Arora A;Agarwal D;Abdel-Fatah TM;Lu H;Croteau DL;Moseley P;Aleskandarany MA;Green AR;Ball G;Rakha EA;Chan SY;Ellis IO;Wang LL;Zhao Y;Balajee AS;Bohr VA;Madhusudan S
• 通讯作者: Madhusudan S

Human RECQL5: guarding the crossroads of DNA replication and transcription and providing backup capability.

• DOI: 10.3109/10409238.2013.792770
• 发表时间: 2013-05
• 期刊: Critical reviews in biochemistry and molecular biology
• 影响因子: 6.5
• 作者: Popuri V;Tadokoro T;Croteau DL;Bohr VA
• 通讯作者: Bohr VA