IMMUNE AND VIRAL CORRELATES OF PEDIATRIC HIV PROGRESSION

儿科艾滋病毒进展的免疫和病毒相关性

• 批准号: 2378586
• 负责人: GWENDOLYN Barbara SCOTT
• 金额: $ 25.23万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-03-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2378586
• 关键词: HIV envelope protein gp120; HIV envelope protein gp160; HIV infections; T cell receptor; antiviral antibody; cell mediated cytotoxicity; cellular immunity; child (0-11); clinical research; cytotoxic T lymphocyte; human immunodeficiency virus 1; human subject; humoral immunity; microorganism immunology; molecular cloning; neutralizing antibody; nucleic acid sequence; pediatric AIDS; radioimmunoassay; virus antigen; virus cytopathogenic effect; virus genetics; virus load

DESCRIPTION: The primary hypothesis under investigation is that humoral and cellular immune responses will affect the biological properties and genetic characteristics of HIV and alter disease progression. The proposed studies will utilize available specimens from a cohort followed longitudinally since 6 months of age or earlier, and focusing primarily on slow progressors and rapid progressors. The Specific Aims of this application are: (1) to study the natural history of humoral immune responses through quantitation of binding antibodies to core and envelope, quantitation of autologous and heterologous neutralizing antibody, and detection of ADCC; (2) to determine the role of cellular immunity in disease progression through characterization of T cell receptor utilization and CTL; (3) to study the biological properties of virus over time as it relates to disease progression by determining viral burden, host cell range and syncytia production.

描述：正在研究的主要假设是体液 细胞免疫反应会影响生物特性 HIV 的遗传特征并改变疾病进展。 这 拟议的研究将利用随后队列中的可用样本 从 6 个月或更早开始纵向，并主要关注 关于缓慢进展者和快速进展者。 本次活动的具体目标 应用有：（1）研究体液免疫的自然史 通过定量结合抗体到核心和 包膜、自体和异源中和的定量 抗体、ADCC检测； (2)确定细胞的作用 通过 T 细胞的表征了解疾病进展中的免疫 受体利用和CTL； (3) 生物学特性研究 病毒随着时间的推移，因为它与疾病进展有关，通过确定病毒 负担、宿主细胞范围和合胞体产生。