IGF::OT::IGF SBIR Phase I Topic 356 Flourogenic assay platform for circular RNA detectionPoP 9/18/2017 - 6/18/2018.

IGF::OT::IGF SBIR 第一阶段主题 356 用于环状 RNA 检测的荧光分析平台PoP 2017 年 9 月 18 日 - 2018 年 6 月 18 日。

• 批准号: 9571847
• 负责人: Balajee Somalinga
• 金额: $ 24.96万
• 依托单位: LUCERNA, INC.
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-18 至 2018-06-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9571847
• 关键词: Basal cell carcinoma; Biochemical; Biological Assay; Cells; Exhibits; Fluorescence; Generations; Glioma; Individual; Length; Ligation; Link; Malignant neoplasm of pancreas; Mediating; Messenger RNA; MicroRNAs; Nature; Oncogenic; Performance; Phase; Porifera; Proteins; RNA; RNA Ligase (ATP); RNA-Binding Proteins; Regulation; Research; Role; Sampling; Scaffolding Protein; Signal Transduction; Small Business Innovation Research Grant; Specificity; Testing; Translations; Tumor Suppressor Proteins; Untranslated RNA; aptamer; base; cancer type; circular RNA; design; diagnostic biomarker; drug discovery; interest; novel; portability; receptor; sensor; tool

Circular RNAs (circRNAs) are a novel class of non-coding RNAs that are an abundant component of the epitranscriptome. Putative functions of circRNAs, include miRNA sequestration, RNA-binding protein scaffolding, mRNA trapping, and protein translation regulation. Deregulation in one or more of these roles has been linked to several types of cancer, including pancreatic cancer, basal cell carcinoma, and gliomas. One well-studied circRNA, ciRS-7, is believed to act as a miRNA-7 sponge and inhibit its regulation of oncogenic receptors and tumor suppressor factors. Despite all the excitement around circRNAs, many of the recently discovered circRNAs remain poorly understood and progress has been extremely slow. An important reason is the lack of easy-to-use tools to study individual circRNA. Recognizing this unmet need, we propose to develop a simple fluorogenic assay platform to detect circRNAs of interest in biochemical and cell lysate-based samples. In phase I, we will develop a circRNA sensor assay that will specifically recognize ciRS-7 and emit fluorescence signal. Due to portable nature of the proposed assay platform, the ciRS-7 sensor can be easily modified to recognize other circRNAs of interest. Once developed, the circRNA sensor platform would find applications in circRNA research, drug discovery, and biomarker diagnostics.

环状 RNA (circRNA) 是一类新型非编码 RNA，是表观转录组的丰富组成部分。 circRNA 的假定功能包括 miRNA 隔离、RNA 结合蛋白支架、mRNA 捕获和 蛋白质翻译调节。这些角色中的一种或多种的放松管制与多种类型的癌症有关， 包括胰腺癌、基底细胞癌和神经胶质瘤。一种经过充分研究的 circRNA，ciRS-7，被认为可以充当 miRNA-7 海绵并抑制其对致癌受体和抑癌因子的调节。尽管所有的 尽管人们对 circRNA 感到兴奋，但许多最近发现的 circRNA 仍然知之甚少，并且进展缓慢 非常慢。一个重要原因是缺乏易于使用的工具来研究个体circRNA。认识到这一点 未满足的需求，我们建议开发一个简单的荧光检测平台来检测生化中感兴趣的circRNA 和基于细胞裂解物的样品。在第一阶段，我们将开发一种 circRNA 传感器检测方法，专门识别 ciRS-7 并发出荧光信号。由于所提出的检测平台的便携性，ciRS-7 传感器可以轻松地安装在 进行修饰以识别其他感兴趣的 circRNA。一旦开发完成，circRNA传感器平台将会找到应用 circRNA 研究、药物发现和生物标志物诊断。