CONSEQUENCES OF LT-ALPHA DEFICIENCY--A MURINE MODEL

LT-α 缺陷的后果——小鼠模型

• 批准号: 2519247
• 负责人: ILA A DAVIS
• 金额: $ 3.53万
• 依托单位: UNIVERSITY OF TENNESSEE KNOXVILLE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2519247
• 关键词: CONSEQUENCES; LT; ALPHA; DEFICIENCY; MURINE

Mice deficient in lymphotoxin alpha (LT-a -/-) have a phenotype characterized by lack of morphologically detectable secondary lymphoid tissue. Specifically- LTa -/- mice lack lymph nodes, Peyer's patches, and their spleens show abnormal organization of the white pulp (1,2,3). Preliminary work indicates that the thymus, as well as thymic and bone marrow development of T and B cell populations, are normal. Immunologically, limited studies indicate inpaired but not absent immune responses. The characterization studies of the LTa -/- mice developed in our laboratory are under review and the manuscript describing such studies is included as an appendix. The most interesting aspect of the LTa -/- mouse is the observation that secondary lymphoid organogenesis is profoundly changed. This should provide a model not only to better understand lymph node development, but also a system to evaluate the role of secondary lymphoid organs for immune induction. The efforts of my investigation will be to a) attempt to further define cellular and molecular events involved in secondary lymphoid development and b) assess the mucosal immunological implications of the absence of Peyer's patches. The initial emphasis will be on the immunological investigations.

缺乏淋巴毒素α（LT-A - / - ）的小鼠具有表型 特征是缺乏形态学检测到的次级淋巴样 组织。 特定于LTA - / - 小鼠缺乏淋巴结，Peyer的补丁， 他们的脾脏表现出白色果肉的异常组织（1,2,3）。 初步工作表明胸腺以及胸腺和骨头 T和B细胞群体的骨髓发育是正常的。 在免疫学上，有限的研究表明被浸入但没有免疫 回答。 LTA - / - 小鼠的表征研究 我们的实验室正在审查，并描述了这样的手稿 研究被包括为附录。 最有趣的方面 LTA - / - 小鼠是次级淋巴机器发生的观察 深刻改变。 这不仅应该为更好 了解淋巴结开发，也是一个评估角色的系统 用于免疫诱导的继发性淋巴机器人。 我的努力 研究将是a）试图进一步定义细胞和 参与二级淋巴发育的分子事件，b）评估 缺乏佩耶斑块的粘膜免疫学意义。 最初的重点将放在免疫学调查上。