CELL COMMUNICATION IN RETINAL MORPHOGENESIS

视网膜形态发生中的细胞通讯

• 批准号: 2403311
• 负责人: JANICE A. FISCHER
• 金额: $ 11.25万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-08 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2403311
• 关键词: Drosophilidae; cell cell interaction; electron microscopy; epitope mapping; gene expression; gene mutation; genetic manipulation; histogenesis; laboratory rabbit; light microscopy; molecular cloning; nucleic acid sequence; ovary; phenotype; protein sequence; protein structure function; retina; visual photoreceptor

The long-term goal of the proposed research is to understand the novel pathway of cell communication in the developing Drosophila eye revealed by the fat facets (faf) gene. faf plays a key role in cell interactions that prevent particular cells present in the earliest stages of eye development from inappropriately becoming photoreceptors, and thus disturbing the symmetry that proper retinal function requires. A combination of molecular and genetic approaches will be used to answer four specific questions. 1. Where is FAF protein expressed and subcellularly localized? Anti-FAF antibodies and epitope-tagged FAF proteins will be generated and used to examine FAF protein expression in developing eyes with both light and electron microscopy. 2. When during eye development is faf function required and in which cells? Heterologous promoters will be used to express faf in portions of the developing eye at different times during development, and the effects on retinal morphogenesis assessed. 3. What are the functional domains of FAF protein? Evolutionarily conserved domains of FAF protein will be identified by isolating the Drosophila virilis faf gene and determining the DNA sequence of its coding region. Also, DNA sequence changes in many mutant faf alleles will be identified. FAF protein domains thus identified will be tested for function in transgenic flies. 4. What are the components of the cell communication pathway in which faf functions? A genetic screen in Drosophila will be conducted to find novel mutations that enhance or suppress the eye defects in faf mutant flies. A screen in yeast for direct protein-protein interactions between FAF protein domains and other eye proteins will be performed. In vertebrates and Drosophila, the use of homologous proteins for cell- cell interactions is commonplace. Thus, although the anatomy of the Drosophila and vertebrate eyes are quite different, the use of Drosophila as a model system will allow the discovery of cell communication mechanisms likely to be common to both.

拟议研究的长期目标是了解小说 发育中的果蝇眼中的细胞通信途径揭示了 由脂肪面（FAF）基因。 FAF在细胞相互作用中起关键作用 这可以防止在眼睛最早的阶段存在特定的细胞 从不恰当地成为感光体的发展，因此 干扰适当的视网膜功能需要的对称性。 一个 分子和遗传方法的组合将用于回答 四个具体问题。 1。FAF蛋白在哪里表达和亚细胞定位？ 将生成抗FAF抗体和表位标记的FAF蛋白，并产生 用于检查两种光发育中的眼睛的FAF蛋白表达 和电子显微镜。 2。当眼睛发育过程中需要FAF功能时， 细胞？ 异源启动子将用于在部分的部分中表达FAF 在开发过程中在不同时间发展眼睛，以及对 评估了视网膜形态发生。 3。FAF蛋白的功能域是什么？ FAF蛋白的进化保守域将通过 隔离果蝇病毒FAF基因并确定DNA 其编码区域的序列。 另外，DNA序列在许多突变体中变化 FAF等位基因将被识别。 因此确定的FAF蛋白结构域将 在转基因蝇中测试功能。 4。细胞通信途径的组成部分是什么 FAF功能？ 将进行果蝇中的遗传筛查以找到新的突变 这可以增强或抑制FAF突变体蝇中的眼睛缺陷。 屏幕 在酵母中，用于直接蛋白质 - 蛋白质相互作用FAF蛋白 将执行域和其他眼蛋白。 在脊椎动物和果蝇中，同源蛋白用于细胞 细胞相互作用很常见。 因此，尽管 果蝇和脊椎动物的眼睛完全不同，果蝇的使用 作为模型系统将允许发现单元通信 机制可能是两者共同的。

项目成果

Genetic interactions with Rap1 and Ras1 reveal a second function for the fat facets deubiquitinating enzyme in Drosophila eye development.

• DOI: 10.1073/pnas.94.23.12515
• 发表时间: 1997-11
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Qinghong Li;I. Hariharan;Fangli Chen;Yongzhao Huang;Janice A. Fischer

Marbles mutants: uncoupling cell determination and nuclear migration in the developing Drosophila eye.

大理石突变体：果蝇眼睛发育中的细胞测定和核迁移的解偶联。

• DOI: 10.1242/dev.120.9.2609
• 发表时间: 1994
• 期刊: Development (Cambridge, England)
• 作者: Fischer-Vize,JA;Mosley,KL
• 通讯作者: Mosley,KL