Mechanism of Candida albicans rupture on biomimetic NSS

仿生NSS上白色念珠菌破裂的机制

• 批准号: 9377579
• 负责人: DENNIS R LA JEUNESSE
• 金额: $ 43.64万
• 依托单位: UNIVERSITY OF NORTH CAROLINA GREENSBORO
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9377579
• 关键词: Adhesions; Alpha Cell; Antibiotic Resistance; Antibiotics; Antifungal Agents; Bacterial Infections; Biological Assay; Biomimetics; Candida albicans; Candidiasis; Cell Adhesion; Cell Wall; Cells; Cellular Morphology; Cellular Structures; Chemicals; Communities; Community Outreach; Confocal Microscopy; Development; Drug resistance; Effectiveness; Environment; Festival; Fungal Drug Resistance; Goals; Growth; Health; Home environment; Hospitals; Human; Immunocompromised Host; Industry; Infection; Joints; Knowledge; Mechanics; Microbe; Microbial Biofilms; Microfluidics; Minority-Serving Institution; Mission; Morbidity - disease rate; Mutation; Mycoses; Nanostructures; North Carolina; Pathogenicity; Play; Research; Research Personnel; Resistance; Resistance development; Role; Rupture; Scanning Electron Microscopy; Schools; Science; Science, Technology, Engineering and Mathematics Education; Structure; Surface; Training; Training and Education; Transplant Recipients; Underserved Population; Universities; Work; Yeasts; antimicrobial; assault; design; doctoral student; extracellular; fungus; insight; killings; methicillin resistant Staphylococcus aureus; microbial; mortality; nanoengineering; nanomaterials; nanoscale; nanoscience; nanostructured; novel; outreach program; programs; resistant strain; response; student training; undergraduate student

Project Summary/Abstract Project Title: Mechanism of Candida albicans rupture on biomimetic NSS PI: Dennis LaJeunesse (UNCG/Nanoscience) The goals of this research are to characterize the nanoscale mechanical interactions between a pathogenic yeast cell, Candida albicans and a cell-rupturing nanostructured surface (NSS) and to define the conditions that determine and control NSS-induced yeast cell rupture. Our long term goals involve the application of NSS’s as a mechanical means of controlling pathogenic fungal growth and biofilm formation. In this proposed research we have three aims: 1) characterize the interactions of C. albicans with a rupturing NSS and determine the roles that cell morphology and adhesion play in NSS induced cell rupture; 2) perform a structural and chemical analysis of the yeast cell during NSS rupture to determine what aspects of the C. albicans cell are vulnerable to NSS assault; and 3) determine how drug resistance strains of C. albicans respond to NSS assault. The proposed research will be the first systematic study of the interactions of a human pathogenic fungi C. albicans and a NSS. Antimicrobial NSS used alone or in conjunction with traditional antifungal drugs have tremendous potential impact for novel antimicrobial treatments. This work will provide greater insight into the fundamental mechanisms of NSS-induced response and practical knowledge that will facilitate the application of NSS fungal control. In addition to the importance of the proposed research, this work will also contribute significantly the central North Carolina Piedmont community as well. The Joint School of Nanoscience and Nanoengineering has a mission to train students, attract industry, and engage the community of the central North Carolina Piedmont. The work will involve the training of doctoral students and several undergraduate researchers in essential STEM programs and will be a major component of community outreach programs such as the JSNN science festival and Nanobus. The University of North Carolina at Greensboro is a minority serving institution and thus this research will also serve to promote STEM education and training to an underserved population.

项目概要/摘要 项目名称：仿生 NSS 上白色念珠菌破裂的机制 PI：Dennis LaJeunesse（UNCG/纳米科学） 这项研究的目标是表征纳米级机械相互作用 致病性酵母细胞、白色念珠菌和细胞破裂纳米结构之间 表面（NSS）并定义确定和控制 NSS 诱导酵母的条件 我们的长期目标涉及将 NSS 作为机械手段进行应用。 在本项研究中控制病原真菌生长和生物膜形成。 我们有三个目标：1）描述白色念珠菌与破裂 NSS 的相互作用 并确定细胞形态和粘附在 NSS 诱导细胞中的作用 2) 在 NSS 破裂过程中对酵母细胞进行结构和化学分析 确定白色念珠菌细胞的哪些方面容易受到 NSS 攻击；3) 确定白色念珠菌的耐药菌株如何响应 NSS 攻击。 拟议的研究将是第一个对人类相互作用的系统研究 病原真菌白色念珠菌和 NSS 单独或联合使用。 与传统抗真菌药物相比，新型抗菌药物具有巨大的潜在影响 这项工作将提供对治疗的基本机制的更深入的了解。 NSS 引起的反应和实践知识将促进 NSS 的应用 除了所提出的研究的重要性之外，这项工作还将。 联合 纳米科学与纳米工程学院的使命是培养学生、吸引 工业，并吸引北卡罗来纳州中部皮埃蒙特社区的参与。 将涉及博士生和几名本科生研究人员的培训 重要的 STEM 计划，并将成为社区外展的主要组成部分 JSNN 科学节和北方大学 Nanobus 等项目。 卡罗莱纳州格林斯博罗分校是一所少数族裔服务机构，因此这项研究也将 致力于向服务不足的人群推广 STEM 教育和培训。