NIDCR DIR Scientific Cores (Combined Technical Research Core)

NIDCR DIR 科学核心（联合技术研究核心）

• 批准号: 10931303
• 负责人: Matthew Hoffman
• 金额: $ 206.85万
• 依托单位: NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10931303
• 关键词: Blood Platelets; Cell Separation; Cells; Cytometry; DNA sequencing; Dental; Disease; Flow Cytometry; Goals; Histology; Hour; Image; Image Cytometry; Imaging Device; National Institute of Dental and Craniofacial Research; Oral; Peripheral Blood Mononuclear Cell; Reaction; Research; Research Personnel; Research Support; Resources; Sampling; Scanning; Services; Sorting; Stains; Students; T-Lymphocyte; Technology; Tissues; Training; cell type; craniofacial; digital; monocyte; operation; tissue processing

The NIDCR DIR Scientific Cores provided research support for intramural investigators performing studies on dental, oral and craniofacial diseases. The Combined Technical Research Core (CTRC)is in its ninth year of operation. CTRC provides access to state-of-the-art research technologies, scientific expertise, and technical support to enhance the research efforts of all NIDCR DIR investigators. The services that it offers include the following: 1) DNA Sequencing; Provided over 1580 reactions for NIDCR DIR investigator-led research. 2) Flow Cytometry; Trained 15 new investigators and students during this period on the Cytometry analyzers, and 5 investigators on the spectral analyzer. Total hours used by investigators was over 2150 hours between the three cytometry analyzers. 3) FACS Cell Sorting; FACS sorted over 600 hours for 19 investigators. 4) Cell Elutriation, have elutriated tota1 of 112 cell types including Monocytes, PBMC, Platelets, and T-Cells. 5) Histology; Performs tissue processing, sectioning and staining for NIDCR investigators working on almost 210 samples/tissues. 6) Image; Have trained over 20 new investigators in the use of ScanScopes, AxioScan, Nanozoomer and CeligoS with the usage of over 1400 hours. CTRC projects increase in demand of its services and resources for the next fiscal year as it has recently trained a few investigators on the Analyzers, Image instruments, and has trained more investigators on the Spectral Analyzer and new Image scanner.

NIDCR DIR科学核心为进行牙科，口服和颅面疾病的研究提供了研究支持。 联合技术研究核心（CTRC）处于运营的第九年。 CTRC提供了对最先进的研究技术，科学专业知识和技术支持的访问，以增强所有NIDCR DIR研究人员的研究工作。它提供的服务包括以下内容： 1）DNA测序；为NIDCR DIR研究者主导的研究提供了1580多种反应。 2）流式细胞仪；在此期间，培训了15名新的研究人员和学生，用于细胞仪分析仪，以及光谱分析仪的5名研究人员。研究人员使用的总小时在三个细胞仪分析仪之间超过2150小时。 3）FACS细胞分类； FACS为19个调查人员分类了600个小时。 4）细胞放大，具有112种细胞类型的tota1，包括单核细胞，PBMC，血小板和T细胞。 5）组织学；为NIDCR研究人员进行近210个样品/组织的NIDCR研究人员进行组织加工，切片和染色。 6）图像；已经培训了20多名新研究人员，用于使用扫描仪，Axioscan，Nanozoomer和Celigos，使用了1400多个小时。 CTRC项目在下一个财政年度对其服务和资源的需求增加，因为它最近培训了一些研究人员的分析仪，图像工具，并培训了更多的调查人员在光谱分析仪和新的图像扫描仪上。