Virus like particles as carriers for carbohydrate based anti-Salmonella vaccines

病毒样颗粒作为碳水化合物抗沙门氏菌疫苗的载体

基本信息

批准号：
9118056
负责人：
Xuefei Huang
金额：
$ 7.68万
依托单位：
MICHIGAN STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-08-01 至 2017-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9118056
关键词：
Alkynes Amides Antibody Response Antigens Azides Bacteria Bacteriophages CD4 Positive T Lymphocytes Carbohydrates Cell surface Characteristics Chemistry Clinic Copper Development Disease Dose Glycoconjugates Health Helper-Inducer T-Lymphocyte Immune response Immunoglobulin G Immunologic Memory Immunology India Investigation Lead Length Multi-Drug Resistance Mus Oligosaccharides Pattern Polysaccharides Public Health Reaction Research Project Grants Resort Route Salmonella Salmonella Vaccines Salmonella enteritidis Salmonella infections Salmonella typhi Salmonella typhimurium Structure Synthesis Chemistry T-Cell Activation Time Tumor-Associated Carbohydrate Antigens Typhoid Fever Ursidae Family Vaccination Vaccine Adjuvant Vaccines Vi capsular polysaccharide Virus-like particle Work base combat cycloaddition design novel novel vaccines pathogenic bacteria prevent programs resistant strain response vaccine development

项目摘要

DESCRIPTION: Salmonella infections (salmonellosis) are a major public health problem throughout the world. In response to RFA-13-179, Indo-U.S. Vaccine Action Program Small Research Grant Program (R03), a collaborative team is formed between two glycoscience groups in the US and India to develop carbohydrate based anti-Salmonella vaccines. Instead of relying on isolating polysaccharides from the pathogenic bacteria, in aim 1, synthetic strategies will be developed to produce structure well defined Salmonella associated glycans from major pathogenic serovars. These glycans will be conjugated with a virus like particle, bacteriophage Qß via state of the art bioconjugation chemistry. In aim 2, the humoral immune responses against these glycoconjugates will be evaluated. As the virus like particle Qß can display the carbohydrate antigens in a highly organized manner and elicit powerful helper T cell activation, it is expected that Qß conjugates can significantly boost the IgG antibody responses against the Salmonella associate glycans and generate glycan specific immunological memory. The abilities of the vaccine constructs to protect against lethal Salmonella challenge will be established. This work can lead to an exciting new direction for anti-Salmonella vaccine development.

描述：沙门氏菌感染（沙门氏菌病）是全世界的一个主要公共卫生问题。为了响应 RFA-13-179、印度-美国疫苗行动计划小型研究资助计划 (R03)，两个糖科学小组之间成立了一个合作小组。美国和印度将开发基于碳水化合物的抗沙门氏菌疫苗，而不是依赖从致病菌中分离多糖，目标 1 将采用合成策略。开发用于从主要致病性血清型中产生结构明确的沙门氏菌相关聚糖，这些聚糖将通过最先进的生物缀合化学与病毒样颗粒、噬菌体 Qß 缀合。在目标 2 中，将评估针对这些糖缀合物的体液免疫反应。由于 Qß 等病毒颗粒可以以高度组织化的方式展示碳水化合物抗原并引发强大的辅助 T 细胞激活，因此预计 Qß 缀合物可以显着增强 IgG 抗体反应对抗沙门氏菌相关聚糖并产生聚糖特异性免疫记忆的能力将得到证实，这项工作可以为抗沙门氏菌疫苗的开发带来令人兴奋的新方向。