Metabolomics Core

代谢组学核心

• 批准号: 9793426
• 负责人: Vinai Chittezham Thomas
• 金额: $ 15.55万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9793426
• 关键词: Address; Affect; Bacterial Physiology; Biochemical; Biological; Carbon; Cells; Charge; Collaborations; Communities; Complement; Computer software; Data; Data Analyses; Detection; Disease; Ensure; Gas Chromatography; Genus staphylococcus; Human Resources; Informatics; Ions; Label; Laboratories; Letters; Maintenance; Mass Fragmentography; Measurement; Metabolic Pathway; Metabolism; Methods; Microbial Biofilms; Noise; Outcome; Pathway interactions; Phenotype; Physiology; Preparation; Procedures; Process; Protocols documentation; Reproducibility; Research; Research Methodology; Resolution; Resources; Sampling; Standardization; Staphylococcus aureus; System; Technology; Time; Tracer; Work; adenylate; base; computerized data processing; cost; design; experience; experimental study; instrument; liquid chromatography mass spectrometry; mass spectrometer; metabolome; metabolomics; pathogen; tool

ABSTRACT In the last two decades, the field of metabolomics has evolved tremendously due to significant advances in analytical technology and informatics. However, these advances have not efficiently been used to augment our understanding of bacterial physiology or host-pathogen interactions. The proposed Metabolomics Core will address this deficiency by assisting the various projects in this PPG that deal with the physiology of Staphylococcus aureus and its interaction with the host. Specifically, the Metabolomics Core will standardize research methodology involved with the extraction of intracellular and excreted metabolites, establish a firm rationale for the interpretation of affected metabolic pathways based on long-standing biochemical principles, design advanced protocols for the detection of unique cellular metabolites and utilize tracer metabolites (e.g. C13- labelled metabolites) to investigate specific metabolic pathways. These aims will be achieved following the acquisition of the state-of-the-art Q Exactive Gas Chromatography (GC-MS/MS) mass spectrometer which enables both targeted and untargeted analyses due to its High-Resolution Accurate Mass (HRAM) Orbitrap technology. The GC-platform will not only complement existing Liquid Chromatography-Mass Spectrometry (LC- MS/MS) based methods currently being utilized by the core but will also uniquely be useful for studies aimed at determining carbon flux measurements through metabolic pathways. We anticipate that the Metabolomics Core will be indispensable for the successful outcome of projects associated with this PPG and further, will be a critical resource for the broader staphylococcal research community.

抽象的 在过去的二十年中，由于代谢组学领域的重大进展，代谢组学领域取得了巨大的发展。 分析技术和信息学。然而，这些进步并没有有效地用于增强我们的能力 了解细菌生理学或宿主-病原体相互作用。拟议的代谢组学核心将 通过协助本 PPG 中涉及生理学的各个项目来解决这一缺陷 金黄色葡萄球菌及其与宿主的相互作用。具体来说，代谢组学核心将标准化 涉及提取细胞内和排泄代谢物的研究方法，建立公司 根据长期存在的生化原理解释受影响的代谢途径的基本原理， 设计先进的方案来检测独特的细胞代谢物并利用示踪代谢物（例如 C13-标记的代谢物）来研究特定的代谢途径。这些目标将在以下情况下实现 收购最先进的 Q Exactive 气相色谱 (GC-MS/MS) 质谱仪， 凭借其高分辨率精确质量 (HRAM) Orbitrap，可实现靶向和非靶向分析 技术。 GC 平台不仅可以补充现有的液相色谱-质谱联用仪 (LC- MS/MS）的方法目前由核心使用，但对于旨在 通过代谢途径确定碳通量测量值。我们预计代谢组学核心 对于与该 PPG 相关的项目的成功结果来说是不可或缺的，并且将是一个关键 为更广泛的葡萄球菌研究界提供资源。