ShEEP Request for BioPlex 3D System

ShEEP 请求 BioPlex 3D 系统

• 批准号: 9796566
• 负责人: William H Robinson
• 金额: --
• 依托单位: VETERANS ADMIN PALO ALTO HEALTH CARE SYS
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9796566
• 关键词: 11 year old; 12 year old; 3-Dimensional; Advisory Committees; Affect; Antibodies; Applications Grants; Automation; Biological; Biological Assay; Biological Markers; Cancerous; Caring; Collaborations; Communicable Diseases; Communities; Computer software; Custom; Diagnosis; Diagnostic; Disease; Equipment; Funding; Funding Agency; Future; Generations; Grant; Human Resources; Individual; Inflammatory; Investigation; Laboratories; Magnetism; Maintenance; Malignant Neoplasms; Measures; Metabolic; Metabolic Diseases; Minor; Modeling; Morphologic artifacts; Pathogenicity; Price; Research; Research Personnel; Research Project Grants; Rheumatoid Arthritis; Running; Sampling; Sheep; Suspensions; System; Technical Expertise; Therapeutic; Time; Tissue Sample; Training; United States National Institutes of Health; Universities; Variant; Vendor; Veterans; automated analysis; base; biomarker development; biomarker discovery; cohort; cytokine; experimental study; instrument; instrumentation; interest; laboratory equipment; member; next generation; novel; novel diagnostics; novel marker; novel therapeutics; preservation; programs; software systems; success; synthetic polymer Bioplex; user friendly software; user-friendly

We request funds to purchase a Bio-Plex 3D Microarray Suspension Array system for biomarker discovery and development at the VA Palo Alto. The requested Bio-Plex 3D will provide significant new capabilities through its ability to simultaneously measure up to 500 analytes per well, increased throughput, and reduced sample requirements. The proposed Bio-Plex 3D will enable analysis of commercially available or in-house developed antibody, multiplex cytokine, and other biomarker assays for investigation of a wide range of inflammatory diseases, cancer, metabolic diseases, and infectious diseases that affect millions of Veterans and civilians. The automated plate washer provides highly consistent washing of the Luminex beads, and is critical for obtaining highly consistent results both within and across experiments. All components included in our request and the attached GSA quote are integral parts of the Bio-Plex 3D System and will not be used independently. The VA Palo Alto research program is in great need of next-generation Luminex-based multiplexing capabilities for VA-funded research projects led by multiple VA Palo Alto-based investigators. The Bio-Plex 3D System will provide significant new multiplexing capabilities, throughput, and sensitivity as well as additional versatility for automated analysis compared to our existing instrument. Our current Luminex 200 System is 12 years old and frequently breaks down from almost daily use by many different VA Palo Alto labs. The requested instrument will provide key new capabilities for a large number of ongoing and future research projects the VA Palo Alto by increasing multiplexing capability, providing higher throughput, preserving limited tissue samples, minimizing intra-panel variation, and increasing the number of projects utilizing multiplex analysis. Similar instruments available at nearby Stanford University are already in high demand, typically saturated, and cannot accommodate the heavy usage by various VA users. Therefore, this instrument will bring significant benefits to the VA Palo Alto Research Community. Compared to similar instruments on the market, the Bio-Plex 3D System has an easier-to-use software system and significantly lower price. The new equipment will be placed in the common laboratory equipment room at the VA Palo Alto, maintained by the Robinson Lab, and available for use by all VA Palo Alto investigators. The Robinson Lab has the required technical expertise for and a track record of setting up, running and maintaining the shared Luminex 200 System at VA Palo Alto, and already established the shared equipment room space, support personnel, and financial plans for the requested instrument. A local advisory committee led by Dr. Robinson and consisting of VA Palo Alto users with a broad range of research interest and expertise will oversee the use and maintenance of the instrumentation. We envision long-term use of the equipment by the Major and Minor users on this grant proposal, in addition to new investigators whose research may require such assay systems. All users have well-established research programs with various sources of funding, including VA, NIH, and other grants, to support these projects. Research at VA Palo Alto is expanding efforts to decipher pathogenic mechanisms underlying diseases that affect Veterans and to develop novel diagnostics and therapeutics. Collaborations among VA Palo Alto laboratories are advancing discovery of next-generation biomarkers that will transform care for Veterans suffering from these diseases. Luminex-based multiplex biomarker analyses are significantly advancing identification of novel biological targets for diagnosis and treatment of these diseases. The requested Bio-Plex 3D System will not only enable collaborative studies at VA Palo Alto, but will also meet the increasing needs of additional projects requiring advanced multiplexing capabilities, throughput, and sensitivity in biomarker analyses.

我们请求资金购买 Bio-Plex 3D 微阵列悬浮阵列系统，用于生物标记物发现和 VA 帕洛阿尔托的开发。所要求的 Bio-Plex 3D 将通过其 每孔能够同时测量多达 500 种分析物，提高通量并减少样品量 要求。拟议的 Bio-Plex 3D 将能够分析市售或内部开发的 抗体、多重细胞因子和其他生物标志物检测，用于研究各种炎症 影响数百万退伍军人和平民的疾病、癌症、代谢性疾病和传染病。这 自动化洗板机可对 Luminex 珠子进行高度一致的清洗，对于获得 实验内和实验间的结果高度一致。我们的请求中包含的所有组件以及 随附的 GSA 报价是 Bio-Plex 3D 系统的组成部分，不能单独使用。 VA Palo Alto 研究计划非常需要下一代基于 Luminex 的多路复用功能 退伍军人事务部资助的研究项目，由多名退伍军人事务部帕洛阿尔托研究人员领导。 Bio-Plex 3D 系统将 提供重要的新复用功能、吞吐量和灵敏度以及额外的多功能性 与我们现有仪器相比的自动化分析。我们当前的 Luminex 200 系统已有 12 年历史， 许多不同的 VA 帕洛阿尔托实验室几乎每天都在使用中经常出现故障。所请求的仪器将 为 VA Palo Alto 的大量正在进行和未来的研究项目提供关键的新功能 提高复用能力，提供更高的通量，保存有限的组织样本，最大限度地减少 组内变异，并增加利用多重分析的项目数量。类似仪器 附近的斯坦福大学的可用资源已经供不应求，通常已经饱和，并且无法 适应各种 VA 用户的大量使用。因此，该工具将为 弗吉尼亚州帕洛阿尔托研究社区。与市场上的同类仪器相比，Bio-Plex 3D 系统 拥有更易于使用的软件系统和显着更低的价格。 新设备将放置在 VA Palo Alto 的公共实验室设备室，维护 由罗宾逊实验室提供，可供所有 VA 帕洛阿尔托调查人员使用。罗宾逊实验室拥有所需的 具有设置、运行和维护共享 Luminex 200 系统的技术专业知识和跟踪记录 在 VA Palo Alto，已经建立了共享设备室空间、支持人员和财务 所需仪器的计划。由 Robinson 博士领导并由 VA Palo 组成的当地咨询委员会 具有广泛研究兴趣和专业知识的 Alto 用户将监督 Alto 的使用和维护 仪器仪表。我们设想主要和次要用户根据本拨款提案长期使用该设备， 除了他们的研究可能需要此类分析系统的新研究人员之外。所有用户均已建立良好的 具有各种资金来源的研究计划，包括 VA、NIH 和其他赠款，以支持这些项目。 弗吉尼亚州帕洛阿尔托的研究正在扩大努力，破译潜在疾病的致病机制 影响退伍军人并开发新的诊断和治疗方法。 VA 帕洛阿尔托之间的合作 实验室正在推进下一代生物标志物的发现，这将改变退伍军人的护理 患有这些疾病。基于 Luminex 的多重生物标志物分析正在显着进步 识别用于诊断和治疗这些疾病的新生物靶点。所要求的Bio-Plex 3D System 不仅可以实现 VA Palo Alto 的协作研究，还可以满足以下领域日益增长的需求： 需要先进的多重功能、通量和生物标志物分析灵敏度的其他项目。