Imaging and Image Analysis Core

成像和图像分析核心

• 批准号: 10627596
• 负责人: Evgenia Dobrinskikh
• 金额: $ 22.55万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627596
• 关键词: Address; Apoptosis; Autophagocytosis; Biological Assay; Cell Aging; Collaborations; Collagen; Complement; Data; Data Analyses; Doctor of Philosophy; Fibrillar Collagen; Fibrosis; Fluorescence; Gene Expression Profile; Goals; Human; Image; Image Analysis; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Individual; Investigation; Label; Lung; Messenger RNA; Mus; Pattern; Phenotype; Proteins; Pulmonary Fibrosis; Research; Resources; Sampling; Services; Specimen; Stains; Structure of parenchyma of lung; data management; design; digital; endoplasmic reticulum stress; improved; lung injury; microscopic imaging; nano-string; programs; second harmonic generation imaging; single-cell RNA sequencing; transcriptome; transcriptome sequencing; whole slide imaging

ABSTRACT The Imaging and Image Analysis Core will perform mRNAs spatial and cellular localization and expression levels analyses, immunofluorescence, as well as quantitative label free fibrosis imaging on human lung tissues-based investigations for each one of the Projects. Samples from specific functional lung regions of extensively phenotyped IPF lungs represent a unique resource that will benefit all three Projects in the Program, and these specimens will need to be carefully stained and imaged for mRNA and protein identification and quantification accumulation to assess ER stress, autophagy, UPR, cell senescence, apoptosis, and fibrosis. Centralization of advanced staining and microscopic imaging will also benefit all Projects by providing quality-controlled data for Project-specific investigations, thus improving the opportunity for inter-project collaboration and integration. This Core is therefore an essential integrative component of the Program. The Aims of the Imaging and Image Analysis Core include: • Design staining combinations and perform regions selection for whole transcriptome analysis with Nanostring GeoMX Digital Spatial Profiler. • Perform RNAscope in situ hybridization and microscopic imaging of IPF and control lung tissue to complement, validate, and contextualize the transcriptional profiles obtained by RNA-seq analyses and to address other project-specific needs. • Perform label-free second harmonic generation imaging of fibrillar collagens for fibrosis quantification. • Perform analyses and quantification of microscopic images. • Provide Digital Whole Slide Images storage in conjunction with the Data Management and Analysis Core (Core D). The Imaging and Image Analysis Core will be led by Evgenia Dobrinskikh, PhD who will coordinate, overview, and perform services for the Scientific Projects and coordinate with other Research Cores.

抽象的 成像和图像分析核心将执行mRNA空间和细胞定位以及表达水平 分析，免疫荧光以及基于人肺组织的定量标签游离纤维化成像 每个项目的调查。广泛的特定功能肺区域的样品 表型的IPF肺代表了一个独特的资源，将使该计划中的所有三个项目都受益，而这些 需要仔细染色和成像，以进行mRNA和蛋白质识别和数量 积累以评估ER应力，自噬，UPR，细胞感应，凋亡和纤维化。集中化 先进的染色和微观成像还将通过提供质量控制的数据来使所有项目受益 特定于项目的调查，从而改善了项目间协作和集成的机会。这 因此，核心是程序的重要集成部分。 成像和图像分析核心的目的包括： •设计染色组合并执行区域选择，以进行整个转录组分析 纳米弦GEOMX数字空间分析师。 •对IPF和控制肺组织进行原位杂交和微观成像 补体，验证和上下文化通过RNA-Seq分析获得的转录曲线和 满足其他特定于项目的需求。 •对原纤维胶原蛋白进行无标签的第二次谐波生成成像，以进行纤维化定量。 •对微观图像进行分析和定量。 •提供数字整体幻灯片图像与数据管理和分析核心结合的存储 （核心D）。 成像和图像分析核心将由Evgenia dobrinskikh，PhD领导，他们将协调，概述， 并为科学项目提供服务，并与其他研究核心协调。