Metabolic basis of mosquito-endosymbiont-virus interactions RBL Admin Supplement

蚊子-内共生体-病毒相互作用的代谢基础 RBL 管理补充品

基本信息

批准号：
10631510
负责人：
Rushika Perera
金额：
$ 37.01万
依托单位：
COLORADO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-03-01 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10631510
关键词：
Acute Administrative Supplement Aedes Arboviruses Award Bacteria Biocontrols Biophysics Blood Vessels Cell Culture Techniques Cells Chikungunya virus Choking Country Culicidae Data Dengue Fever Dengue Hemorrhagic Fever Dengue Shock Syndrome Dengue Virus Diagnostic Energy Metabolism Extravasation Family Fever Flaviviridae Funding Genome Goals Grant Heritability Human In Vitro Infection Insecta Investigation Laboratories Life Life Cycle Stages Lipids Lipolysis Maintenance Mediating Membrane Metabolic Metabolic Pathway Methods Modeling Modification National Institute of Allergy and Infectious Disease Outcome Parents Pathway interactions Persons Phenotype RNA Viruses Research Resources Risk Serotyping Site Sphingolipids Structure Syndrome Therapeutic Tissues Translations United States National Institutes of Health Vaccinee Vaccines Viral Viral Physiology Viral Proteins Virion Virus Virus Replication Wolbachia World Health Organization Zika Virus antagonist biophysical analysis chikungunya infection density endosymbiont fitness insight interest lipid biosynthesis metabolic phenotype metabolome mitochondrial metabolism particle pathogen success symbiont transmission process vector vector mosquito viral transmission

项目摘要

PROJECT SUMMARY / ABSTRACT This supplement is responsive to the Notice of Special Interest, NOT-AI-22-049, “Administrative Supplements for NIAID Regional Biocontainment Laboratories (RBL) and other NIH Grantees Conducting Research at RBL- affiliated Sites” and the scope of our NIAID grant, (fund R01AI151166), entitled, “Metabolic basis of mosquito- endosymbiont-virus interactions.” Here, we will accelerate and advance Specific Aims 1-3 of the parent award through two primary supplementary aims; SA 1-3.supp1: Assess how modification of cellular lipids alters the composition of the viral envelope and required structural transitions for maturation and infectivity in Ae. Aegypti. The data generated from SA 1-3.supp1 will significantly enhance the parent award data outcomes as it provides biophysical insight into how lipid modulation might alter virus structure and function and thus impact the viral life cycle in Ae. aegypti. SA 2.supp2: Assess how Wolbachia-mediated lipid changes alter ZIKV and CHIKV infection and virus-associated lipid modulations. The data generated from SA 2.supp2 will significantly enhance the parent award data outcomes by providing additional insight into the metabolic state of the vector following infection with all three vectored viruses. The studies will enhance our understanding of metabolic choke points that may be applicable to all three viruses, and/or significant differences that might hinder biocontrol efforts. Both efforts synergize with the goals of the RBL in (diagnostics, therapeutics, vaccines, and transmission control methods for Arboviruses vectored by Ae. aegypti); and has the full support of the Scientific Director of the RMRBL.

项目摘要 /摘要这种补充对特殊关注的通知有回应，而不是-AI-22-049 用于NIAID区域生物疗养实验室（RBL）和其他NIH授予者，在RBL- 附属站点”和我们的NIAID赠款的范围（基金R01AI151166），题为“蚊子的代谢基础” 内共生病毒相互作用。通过两个主要的补充目标；病毒包膜的组成和所需的结构质量，以使AE成熟和感染。 SA 1-33产生的数据将显着增强父奖的结果。关于脂质调节如何改变病毒结构和功能，从而影响病毒寿命的生物物理洞察力在AE中循环。和与病毒相关的脂质模块化。通过提供附加洞察力来授予数据结果这三种矢量病毒将增强我们对代谢chololic cholocal cholocy Choint的理解适用于所有三个纯属，或/或可能阻碍生物防治的显着差异。与RBL的目标协同作用（诊断，治疗学，疫苗和传输控制方法对于Ae载体的Arboviruse。