B Cell/T Cell Interactions in Brucellosis

布鲁氏菌病中 B 细胞/T 细胞的相互作用

• 批准号: 10630491
• 负责人: Jerod Skyberg
• 金额: $ 5.01万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-11-18 至 2023-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630491
• 关键词: Affect; Animals; Anti-Inflammatory Agents; Antibiotic Therapy; Antibodies; Antigen Presentation; Antigen-Presenting Cells; Award; B-Lymphocytes; Brucella; Brucella Vaccine; Brucellosis; CD4 Positive T Lymphocytes; Cell Communication; Cell Separation; Cell physiology; Cells; Chronic; Complement; Control Animal; Data; Disease; Fever; Future; Gene Expression Profiling; Genetic Transcription; Goals; Human; Immunity; Impairment; Infection; Knowledge; Laboratories; Ligands; Mediating; Missouri; Modeling; Mus; Pathogenesis; Pathway interactions; Predisposition; Public Health; Relapse; Role; T cell response; T-Lymphocyte; Universities; Vaccinated; Vaccination; Vaccine Design; Vaccines; Virulent; Zoonoses; cytokine; design; improved; neglect; prevent; rational design; receptor; response; transcriptome sequencing; vaccine access; vaccine development

Project Summary/Abstract In humans, Brucella spp. can cause a lifelong, debilitating disease, with relapses of undulating fever and other complications even with antibiotic treatment. No vaccines are currently licensed to prevent human brucellosis. In this focused Supplement, we will employ the BSL3 cell sorting capability of the University of Missouri Regional Biocontainment Laboratory (MURBL) along with RNA-seq to perform detailed transcriptional analyses of B and T cells in our model of vaccine-mediated immunity to Brucella. Our findings from this Supplement will complement the results from our parental proposal and provide us additional information which will enhance our understanding of how B cell antigen presentation inhibits vaccine-mediated immunity against Brucella, which in turn could improve the rational design of vaccines for brucellosis.

项目摘要/摘要 在人类中，布鲁氏菌。会引起终生，使人衰弱的疾病，并带有起伏的发烧和 即使接受抗生素治疗，其他并发症也是如此。目前没有疫苗可以预防人类 布鲁氏菌病。在这种重点补充剂中，我们将采用大学的BSL3细胞分类能力 密苏里州区域生物疗养实验室（MURBL）以及RNA-Seq进行详细的转录 在我们的疫苗介导的布鲁氏菌免疫模型中，B和T细胞的分析。我们的发现 补充将补充我们父母提案的结果，并为我们提供其他信息 将增强我们对B细胞抗原表现如何抑制疫苗介导的免疫力的理解 布鲁氏菌，反过来可以改善疫苗的布鲁氏菌病的合理设计。