Mouse Support Core

鼠标支撑核心

• 批准号: 10625321
• 负责人: Ulrike Lorenz
• 金额: $ 39.77万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10625321
• 关键词: Address; Animal Model; Animals; Blood Vessels; Breeding; Cardiovascular Diseases; Cells; Communities; Databases; Dedications; Disease; Ensure; Equipment and supply inventories; Funding; Genes; Genotype; Goals; Individual; Inflammation; Inflammatory; Knock-out; Knockout Mice; LoxP-flanked allele; Maintenance; Mediating; Mission; Modeling; Mouse Strains; Mus; Mutation; Physiology; Proteins; Research Personnel; Resources; STK11 gene; Site; Source; Support System; Time; Tissues; Transgenes; Transgenic Mice; Transgenic Organisms; Variant; Work; base; cost; genetic regulatory protein; human disease; innovation; mouse model; mutant; overexpression; programs; success; tool; transgene expression

CORE B PROJECT SUMMARY The primary goals of this Program Project application are to understand the function of pannexin channels in normal physiology, as well as well as under conditions of cardiovascular and inflammatory diseases. This Mouse Support Core/Core B will serve all projects in the following ways: (1) This core will provide the husbandry, genotyping and basic crossing of conditional Panx1 knockout mice to specific Cre strains; specifically, the Panx1fl/fl mice crossed to at least five different Cre strains that are relevant to studying cardiovascular and inflammatory diseases. (2) This Core will also help generate additional transgenic Panx1 mice for tissue-specific expression of Panx1-WT and variants. (3) The Core will generate Cre-mediated tissue-speficic knockouts of the Panx1-regulatory proteins Sik1 and LKB1. The key benefits of this Mouse Support Core are that it will help consolidate the breeding and mouse maintenance, save time and cost, and avoid variations due to colonies individually maintained by the four project leaders. As the previous funding period has shown, having a dedicated core to cross the various Cre strains will be a huge advantage to all four projects. Moreover, the innovative transgenic mouse models overexpressing wild type or mutant forms of Panx1 will help answer key questions posed in the four main Projects in additional unique ways. Collectively, these mouse strains/models and analyses tools generated are expected to be fundamentally important in advancing our work to human disease, and will also help the larger community of researchers studying pannexin channel function pertaining to many human diseases.

核心 B 项目摘要 该计划项目申请的主要目标是了解 pannexin 通道的功能 正常生理，以及心血管和炎症疾病的情况下。这只鼠标 支持核心/核心B将通过以下方式为所有项目提供服务：（1）该核心将提供畜牧业， 条件 Panx1 敲除小鼠与特定 Cre 品系的基因分型和基本杂交；具体来说， Panx1fl/fl 小鼠与至少五种不同的 Cre 品系杂交，这些品系与研究心血管和 炎症性疾病。 (2) 该核心还将有助于生成额外的转基因 Panx1 小鼠，用于组织特异性 Panx1-WT 及其变体的表达。 (3) Core 将产生 Cre 介导的组织特异性敲除 Panx1 调节蛋白 Sik1 和 LKB1。该鼠标支持核心的主要优点是它可以帮助 集中饲养和小鼠维护，节省时间和成本，避免由于菌落引起的变异 由四位项目负责人单独维护。正如上一个资助期所示，有一个专门的 交叉各种 Cre 菌株的核心将为所有四个项目带来巨大优势。此外，创新的 过度表达 Panx1 野生型或突变型的转基因小鼠模型将有助于回答关键问题 以其他独特的方式在四个主要项目中提出。总的来说，这些小鼠品系/模型和分析 预计所产生的工具对于推进我们针对人类疾病的工作具有根本性的重要作用，并且将 还可以帮助更多的研究人员研究与许多人类相关的 pannexin 通道功能 疾病。