LC-Orbitrap MS/MS System for shotgun Proteomics at Dartmouth

达特茅斯用于鸟枪蛋白质组学的 LC-Orbitrap MS/MS 系统

• 批准号: 8447223
• 负责人: Scott A. Gerber
• 金额: $ 60万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447223
• 关键词: Biomedical Research; Cell Cycle Regulation; Circadian Rhythms; Communities; Faculty; Funding; Future; Goals; Housing; Human; Malignant neoplasm of lung; Norris Cotton Cancer Center; Proteomics; Proteomics Shared Resource; Research; Research Project Grants; Secure; Shotguns; Signal Transduction; System; United States National Institutes of Health; Work; college; instrumentation; insulin signaling; mass spectrometer; medical schools; pathogen; research study

DESCRIPTION (provided by applicant): The objective of this proposal is to secure funding for a microcapillary LC-LTQ Orbitrap Elite mass spectrometer system to support shotgun proteomics-oriented, NIH-funded scientific goals of the greater Dartmouth biomedical research community. The users of the proposed system described in this application come from a broad range of departments and centers at Dartmouth College and Dartmouth Medical School, including the NCI-designated Norris Cotton Cancer Center (NCCC). Their projects represent a diverse continuum of research foci, from basic cellular mechanisms of cell cycle control and circadian rhythms to mechanisms of host-pathogen interactions and insulin signaling, to more translational projects aimed at understanding how cellular signaling is dysregulated in human lung cancers. Collectively, these research projects represent work and effort that has exceeded the capacity of the single LC-MS/MS platform that exists in all of Dartmouth for such experiments. Thus, the acquisition of a new mass spectrometer into the Dartmouth Proteomics Shared Resource is critically important to enable the completion of current, as well as future, NIH research goals of our faculty. The proposed instrumentation will be housed in our Proteomics Shared Resource, which is maintained by NCCC on behalf of the greater Dartmouth community.

描述（由申请人提供）：本提案的目的是为微毛细管 LC-LTQ Orbitrap Elite 质谱仪系统争取资金，以支持大达特茅斯生物医学研究界以鸟枪法蛋白质组学为导向、由 NIH 资助的科学目标。本申请中描述的拟议系统的用户来自达特茅斯学院和达特茅斯医学院的各个部门和中心，包括 NCI 指定的诺里斯·科顿癌症中心 (NCCC)。他们的项目代表了不同的研究重点，从细胞周期控制和昼夜节律的基本细胞机制到宿主-病原体相互作用和胰岛素信号传导的机制，再到旨在了解人类肺癌中细胞信号传导如何失调的更多转化项目。总的来说，这些研究项目所代表的工作和努力已经超出了达特茅斯所有用于此类实验的单一 LC-MS/MS 平台的能力。因此，在达特茅斯蛋白质组学共享资源中购置一台新的质谱仪对于完成我们教师当前和未来的 NIH 研究目标至关重要。拟议的仪器将存放在我们的蛋白质组学共享资源中，该资源由 NCCC 代表达特茅斯社区维护。

项目成果

The iron deficiency response in Arabidopsis thaliana requires the phosphorylated transcription factor URI.

拟南芥的缺铁反应需要磷酸化转录因子 URI。

• 发表时间: 2019
• 影响因子: 11.1
• 作者: Kim, Sun A;LaCroix, Ian S;Gerber, Scott A;Guerinot, Mary Lou
• 通讯作者: Guerinot, Mary Lou