Programmable evolution of optogenetic systems

光遗传学系统的可编程进化

• 批准号: 10623650
• 负责人: Todd P Coleman
• 金额: $ 2.29万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10623650
• 关键词: 3D Print; Biochemical; Biological; Biological Process; Biomedical Research; Cells; Cellular biology; Chemicals; Color; Developmental Biology; Educational process of instructing; Escherichia coli; Ethics; Event; Evolution; Fluorescence; Genes; Genetic; High School Student; In Vitro; Learning; Light; Location; Manuscripts; Measures; Minor; Modification; Mutation; Optics; Process; Proteins; Publications; Publishing; Research; Research Training; Schools; Speed; Stimulus; Students; System; Techniques; Testing; Time; Training; Writing; Yeasts; base; career; experience; imaging study; materials science; mutant; novel; novel strategies; optogenetics; parent grant; posters; programs; skills; success; symposium; tool; undergraduate research; undergraduate student

Project Summary: This project is specifically tailored for the candidate, Nhu Dao, to conduct biomedical research. Nhu’s research involves evolving novel proteins that can be photo-cleaved using light, allowing for uses in biological imaging, studying cellular and developmental biology, optically controlled 3D printing, and more. Using a system that enables a gene to continuously evolve, only cells containing a gene that responds to specific wavelengths (colors) of light will grow. This process can rapidly evolve this new class of proteins at an unprecedented pace. Proteins resulting from this evolution will be characterized using techniques from an undergraduate “Biochemical Techniques” course previously taught by the PI. The project uses hardware from the parent grant with minor modifications, making the timing and feasibility ideal for undergraduate research. In addition to the research training experience, the candidate will undergo extensive training in ethics, project management, learning/teaching-related skills, writing, presentation skills, interpersonal skills, and more. This training will initially take place in a “boot camp” setting and then be expanded upon throughout the year. Finally, this training and research will culminate in writing about the research in manuscript form and in a poster that will be presented in several poster symposia. Combined, this experience will boost the candidate’s career, preparing her for success in biomedical research in a 4-year program, graduate school, and beyond.

项目概要：该项目专门为候选人Nhu Dao量身定制，进行生物医学研究 Nhu 的研究涉及进化出可以利用光进行光裂解的新型蛋白质。 用于生物成像、研究细胞和发育生物学、光控 3D 打印以及 使用使基因能够持续进化的系统，只有含有基因的细胞才会做出反应。 特定波长（颜色）的光会生长，这个过程可以快速进化出这类新的蛋白质。 将使用以下技术以前所未有的速度对这种进化产生的蛋白质进行表征。 之前由 PI 教授的本科生“生化技术”课程该项目使用硬件。 来自家长的资助，稍作修改，使时间和可行性成为本科生的理想选择 研究。 除了研究培训经验外，候选人还将接受广泛的道德培训， 项目管理、学习/教学相关技能、写作、演示技能、人际交往能力，以及 该培训最初将在“新兵训练营”环境中进行，然后在整个培训中进行扩展。 最后，培训和研究将以手稿形式和形式撰写研究成果。 一张海报将在多个海报研讨会上展示，这种体验将促进 候选人的职业生涯，为她在四年制项目、研究生院、生物医学研究方面取得成功做好准备， 以及更远的地方。