NITRIC OXIDE RELEASING BIOMIMETRIC NANOMATRIX GELF OR ROOT REVITALIZATION

释放一氧化氮的仿生纳米基质凝胶或根部活化

• 批准号: 10623370
• 负责人: Kyounga Cheon
• 金额: $ 13.29万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10623370
• 关键词: Anti-Bacterial Agents; Antibiotics; Biomimetics; Cervical; Clinic Visits; Clinical; Clinical Research; Composite Resins; Dental Pulp; Dental crowns; Dentin; Development; Disinfection; Endodontics; Environment; Enzymes; Excision; Exposure to; Extracellular Matrix; Financial Hardship; Fracture; Future; Gel; Hemorrhage; Infection; Inflammatory Response; Lead; Mechanics; Mediating; Methods; Microbial Biofilms; Minocycline; Modeling; Natural regeneration; Necrosis; Nitric Oxide; Outcome; Patients; Plant Roots; Procedures; Process; Public Health; Pulp Canals; Rattus; Sampling; Structure; Tissues; Tooth Discoloration; Tooth structure; Trauma; Treatment Protocols; Treatment outcome; Visit; biomaterial compatibility; calcium hydroxide; cost effective; innovation; periapical; prevent; recruit; regenerative; restoration; stem cells; tissue repair

PROJECT SUMMARY Dental pulp tissue exposed to a mechanical trauma or cariogenic process can result in root canal and/or periapical infections, which can be treated via an endodontic procedure. A regenerative endodontic procedure (REP) attempts to revitalize pulp-dentin tissue from a previously necrotic or inflamed pulp, thus, allowing for continued development of the pulp-dentin structure, particularly for a young immature root. However, the current REP has resulted in unfavorable outcomes, including tooth discoloration, cervical root fractures, inadequate pulp-dentin tissue structure formation, and multiple clinic visits. To tackle the challenges of the current REP, a highly interdisciplinary and innovative strategy is proposed for efficient root canal disinfection and pulp-dentin tissue revitalization using an antibiotics and nitric oxide (NO) releasing biomimetic nanomatrix gel. We hypothesize that the antibiotics and NO releasing nanomatrix gel will demonstrate an efficient antibacterial effect and recruit endogenous stem cells to induce pulp-dentin revitalization, while providing a favorable pulp-dentin tissue mimicking extracellular matrix environment. The proposed strategy has several advantages over the current REP: 1) preventing tooth discolorations by removal of minocycline, 2) preventing cervical root fractures by avoidance of calcium hydroxide, 3) antibacterial effect of the NO, 4) sustained NO release from the nanomatrix gel by enzyme mediated degradation, 5) reducing inflammatory responses by avoidance of reopening root canals and stimulated bleeding, 6) biocompatibility and biodegradability of the nanomatrix gel as a functional extracellular matrix, and 7) less extensive pulp access opening, clinical visits, and restorations with cost-effective materials such as composites resin. Therefore, three specific aims are proposed to evaluate the hypothesis. Specific Aim 1 is to evaluate the anti-biofilm effects of the antibiotics and NO releasing biomimetic nanomatrix gel in clinical samples from the endodontic infection. Specific Aim 2 is to evaluate the cellular effects of the antibiotics and NO releasing biomimetic nanomatrix gel on dental pulp stem cells. Specific Aim 3 is to develop a rat infected tooth model and evaluate the revitalization capacity of the antibiotics and NO releasing biomimetic nanomatrix gel. The outcomes from this proposal will demonstrate the anti-biofilm effect and revitalization potential of the antibiotics and NO releasing nanomatrix gel for the treatment of endodontic infections. Further, the outcomes will also lead to future clinical studies for a young immature root as well as a mature root canal infection with traumatic exposure and bacterial associated cases.

项目摘要 暴露于机械创伤或致癌过程的牙髓组织会导致根管和/或 周围感染，可以通过牙髓方法治疗。再生牙髓方法 （REP）试图从先前坏死或发炎的纸浆中振兴牙髓丁丁组织，从而允许 继续开发纸浆丁丁结构，特别是对于年轻的不成熟根。但是，电流 REP导致不利的结果，包括牙齿变色，颈根骨折，不足 纸浆丁丁组织结构的形成和多次临床就诊。为了应对当前代表的挑战， 提出了高度跨学科和创新策略，以进行有效的根管消毒和纸浆底漆 使用抗生素和一氧化氮（NO）释放仿生纳米纳米质凝胶的组织振兴。我们 假设抗生素和没有释放的纳米疗法凝胶将表现出有效的抗菌作用 并募集内源性干细胞诱导纸浆丁丁素振兴，同时提供有利的纸浆丁丁素 模拟细胞外基质环境的组织。提出的策略比 当前代表：1）通过去除米诺环素防止牙齿变色，2）防止颈根骨折 通过避免氢氧化钙，3）NO，4）不释放纳米疗法的抗菌作用 通过酶介导的降解凝胶，5）通过避免重新开放根管来减少炎症反应 和刺激的出血，6）纳米瘤凝胶的生物相容性和生物降解性作为功能 细胞外基质和7）较少广泛的纸浆接入，临床访问和具有成本效益的修复 诸如复合材料树脂之类的材料。因此，提出了三个特定目标来评估该假设。 具体目的1是评估抗生素的抗生物胶片效应，无释放的仿生纳米疗法 来自牙髓感染的临床样品中的凝胶。具体目标2是评估 抗生素，无释放牙浆干细胞上的仿生纳米疗法凝胶。特定目标3是发展 大鼠感染的牙齿模型并评估抗生素的振兴能力，没有释放仿生剂 Nanomatrix凝胶。该提案的结果将证明抗生物膜的效应和振兴 抗生素的潜力和无释放的纳米质凝胶用于治疗牙髓感染。更远， 结果还将导致未来的年轻未成熟根和成熟根管的临床研究 感染创伤性暴露和细菌相关病例。

项目成果

Biocompatibility and mineralization potential of new calcium silicate cements.

• DOI: 10.1016/j.jds.2022.10.004
• 发表时间: 2023-07
• 期刊: JOURNAL OF DENTAL SCIENCES
• 影响因子: 3.5
• 作者: Kim, Byurira;Lee, Yong-Hyuk;Kim, Ik-Hwan;Lee, Ko Eun;Kang, Chung-Min;Lee, Hyo-Seol;Choi, Hyung-Jun;Cheon, Kyounga;Song, Je Seon;Shin, Yooseok
• 通讯作者: Shin, Yooseok

Establishment of quantitative indicators for an efficient treatment on masticatory muscle pain.

• DOI: 10.1002/cre2.705
• 发表时间: 2023-02
• 期刊: CLINICAL AND EXPERIMENTAL DENTAL RESEARCH
• 影响因子: 1.8
• 作者: Lee, Sunhee;Ju, Hye-Min;Ho, Donald;Song, Byong-Sop;Jeong, Sung-Hee;Ahn, Yong-Woo;Ok, Soo-Min;Cheon, Kyounga
• 通讯作者: Cheon, Kyounga

Doxycycline-Loaded Nitric Oxide-Releasing Nanomatrix Gel in Replanted Rat Molar on Pulp Regeneration.

负载多西环素的一氧化氮释放纳米基质凝胶用于再植大鼠磨牙的牙髓再生。

• DOI: 10.3390/app11136041
• 发表时间: 2021
• 期刊: Applied sciences (Basel, Switzerland)
• 作者: Yun,Kwan-Hee;Ko,Mi-Ja;Chae,Yong-Kown;Lee,Koeun;Nam,Ok-Hyung;Lee,Hyo-Seol;Cheon,Kyounga;Choi,Sung-Chul
• 通讯作者: Choi,Sung-Chul

Candida Infection Associated with Salivary Gland-A Narrative Review.

• DOI: 10.3390/jcm10010097
• 发表时间: 2020-12-30
• 期刊: Journal of clinical medicine
• 影响因子: 3.9
• 作者: Ok SM;Ho D;Lynd T;Ahn YW;Ju HM;Jeong SH;Cheon K
• 通讯作者: Cheon K

Evaluation of children's pain expression and behavior using audio visual distraction.

• DOI: 10.1002/cre2.407
• 发表时间: 2021-10
• 期刊: Clinical and experimental dental research
• 影响因子: 1.8
• 作者: Delgado A;Ok SM;Ho D;Lynd T;Cheon K
• 通讯作者: Cheon K