A New Paradigm to Treat Bleeding by Augmenting Hemostasis via Microscale Electrical Fields

通过微尺度电场增强止血来治疗出血的新范例

基本信息

批准号：
10612469
负责人：
Eudorah Vital
金额：
$ 5.27万
依托单位：
EMORY UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10612469
关键词：
Acceleration Address Adhesives Adverse effects Appearance Bandage Basic Science Behavior Biological Biological Products Biological Sciences Biomedical Engineering Blood Blood Vessels Blood coagulation Cell Adhesion Molecules Cells Chemical-Induced Change Chemicals Chemistry Clinical Communication Data Deposition Development Plans Device or Instrument Development Devices Diameter Disease Electrical Engineering Electronics Electrosurgery Endothelial Cells Endothelium Engineering Environment Exposure to Fibrin Fibrinogen Fostering Foundations Future Generations Geometry Goals Health Hematological Disease Hematology Hemorrhage Hemostatic Agents Hemostatic function Human Immune In Vitro Intervention Investigation Kinetics Laboratories Lead Literature Measures Mechanics Medical Mentorship Methods Microfluidics Microscopy Modality Modeling Morbidity - disease rate Nature Operative Surgical Procedures Paper Patients Physicians Physiology Polymers Public Health Publishing Reactive Oxygen Species Research Resources Scientist Techniques Temperature Therapeutic Thrombin Thromboplastin Time Tissue Preservation Tissues Training Trauma Up-Regulation Vascular Endothelium Work cost effective design electric field improved in vitro Model in vivo in vivo Model infection risk innovation insight meter microelectronics microsystems mortality nanofabrication new technology novel novel strategies pharmacologic polymerization response targeted treatment therapy development tool voltage wound

项目摘要

PROJECT SUMMARY/ABSTRACT Hemorrhage is a common occurrence inside and outside of the clinical environment. Although advancements have been made to enable rapid hemostatic control, hemorrhage is still a significant contributor to morbidity and mortality. Current approaches to achieve hemostatic control focus on pharmacological and electrothermal means of intervention. These methods of addressing hemorrhage are effective; however, they are not broadly applicable and are associated with numerous adverse effects. The pharmacological methods rely on the use of biologic agents that present the risks of infection and immune dysregulation. Electrothermal means of addressing hemorrhage, such as electrocauterization often result in compromised tissue appearance and function. To address this need for improved hemostatic agents, we propose the use of microscale electrical fields to accelerate hemostasis, which has been demonstrated by our previous work. A fundamental question pertains to the mechanistic underpinnings of microscale-electrical-field hemostatic augmentation. The central hypothesis is that tunable (low voltage) electrical fields catalyze pro-hemostatic fibrin deposition and endothelial mechanics. This hypothesis will be investigated by the following proposed specific aims. Aim 1 will establish the mechanism underlying microscale-electrical-field hemostatic augmentation. The objective is to understand how microscale electrical fields target hemostasis in comparison to current hemostatic agents. Aim 2 will use novel in vitro models of blood vessels to characterize how blood vessel cells respond to electrical fields in the context of bleeding. The trainee will master a wide range of engineering, chemistry, and biological techniques, including nanofabrication, microsystem engineering using microfluidics, and advanced microscopy techniques. The laboratory in which the proposed work will be conducted is the ideal environment for this research trainee as it has demonstrated an abundance of resources and numerous opportunities for cross-training in fields ranging from life sciences to engineering all of which will foster the well-roundedness of the trainee. The proposed research will provide insight into a novel approach to accelerating hemostasis using microscale electrical fields. This research will provide a strong foundation for future medical microsystem- based strategies for evaluating disease and treatment options. The training plan proposed to accomplish these goals has been specifically designed to provide the PI with the environment, training, and mentorship necessary to succeed as a physician- scientist-engineer.

项目摘要/摘要出血是临床环境内外的常见发生。虽然进步已经实现了快速的止血控制，出血仍然是发病率和死亡。目前的方法以实现止血控制关注药理学和电热均值干预。这些解决出血的方法是有效的。但是，它们不是广泛适用的并与许多不利影响有关。药理学方法依赖于生物学的使用出现感染和免疫失调风险的药物。电热的方法出血，例如电载体，通常会导致组织外观和功能受损。到解决了改善止血剂的需求，我们建议使用微观电场加速止血，这是我们以前的工作证明的。一个基本问题与显微镜 - 电场止血增强的机械基础。中心假设是可调节的（低压）电场催化促纤维蛋白沉积和内皮力学。该假设将通过以下提出的特定目的进行研究。 AIM 1将建立机制基础显微镜 - 电场止血增强。目的是了解微观与电流止血剂相比，电场靶向止血。 AIM 2将在体外使用小说血管模型以表征血管细胞如何在流血。学员将掌握广泛的工程，化学和生物技术，包括纳米机器化，使用微流体和高级显微镜技术的微系统工程。这将进行拟议工作的实验室是该研究学员的理想环境已经证明了丰富的资源和许多在范围内交叉训练的机会从生命科学到工程，所有这些都将促进学员的全面性。提议研究将提供对使用微观电场加速止血的新方法的见解。这项研究将为未来的医学生理系统评估策略提供良好的基础疾病和治疗选择。为实现这些目标而提出的培训计划是专门的旨在为PI提供成功的环境，培训和指导，以成功作为医生 - 科学家工程师。