Central Laboratory Core

中心实验室核心

• 批准号: 10598378
• 负责人: Johann Wilhelm Schneider
• 金额: $ 18.71万
• 依托单位: H. LEE MOFFITT CANCER CTR & RES INST
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-03 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10598378
• 关键词: Anus; Biological Assay; Biological Specimen Banks; CDKN2A gene; Cancer Burden; Cancer Center; Characteristics; Classification; Collaborations; Collection; Complement; Country; DNA; Databases; Development; Diagnosis; Ensure; Etiology; Evaluation; Future; Genetic Transcription; Genotype; German population; Germany; Goals; Guidelines; HIV; Histology; Human Herpesvirus 4; Human Papilloma Virus-Related Malignant Neoplasm; Human Papillomavirus; Image; Immune; Immunofluorescence Immunologic; Immunohistochemistry; Immunologic Tests; Immunologics; Infrastructure; Institution; International; Intervention; Investigation; Knowledge; Laboratories; Leadership; Malignant Neoplasms; Methodology; NCI Center for Cancer Research; Oncology; Oropharyngeal; Pathologic; Pathologist; Pathology; Patients; Persons; Pilot Projects; Policies; Prevention; Procedures; Process; Quality Control; RNA; Research; Research Personnel; Resources; Sampling; Science; Serology; Site; Slide; South Africa; Specimen; Technology; Testing; Tissue Banks; Tissues; Tumor-infiltrating immune cells; Universities; Viral; Viral Cancer; Vulva; Zimbabwe; adjudication; biobank; biological specimen archives; cancer therapy; design; digital; experience; flexibility; low and middle-income countries; penis; translational study; tumor; tumor microenvironment; viral detection; virtual; virus related cancer

PROJECT SUMMARY – CENTRAL LABORATORY CORE The overarching goal of the Central Laboratory (CL) Core for the Partnership to Assess Viral and Immune Landscape Intersections with Oncology for People Living with HIV (PAVILION) is to provide centralized biobanking and virologic and immunologic assessment of well annotated biospecimens in support of the proposed translational studies as well as future PAVILION pilot projects. The CL Core will be co-led by three exceptional investigators, Drs. Johann Schneider (South Africa), Zvavahera (Mike) Chirenje (Zimbabwe), and Tim Waterboer (Germany), who have complementary expertise. The CL Co-Leaders have designed a robust yet flexible pipeline and range of analytical methodologies that builds upon the infrastructure and long-term international collaborations established by the PAVILION partnering investigators in South Africa, Zimbabwe, the US, and Germany. The CL Core purposefully pairs this unique expertise with established laboratories in the low- and middle-income country (LMIC) partner countries to efficiently advance knowledge related to tumor and patient characteristics. This knowledge can then inform the development of effective prevention and cancer treatment interventions to reduce the burden of cancer for people living with HIV (PLWH) in LMICs disproportionately impacted by HIV. To achieve the overarching goal, the CL Core will provide centralized, research-quality pathology evaluation for endpoint adjudication of collected tissues, DNA and RNA extraction, HPV and EBV detection and genotyping, RNA expression analysis, multiplex viral serology assessment, and immunological evaluation of the tumor microenvironment for the proposed and future projects. These activities are organized into the following Aims: Aim 1: Serve as a centralized biobanking resource in South Africa and Zimbabwe for the collection, processing, and storage of biospecimens in support of PAVILION studies This aim provides biobanking of high-burden and under-researched viral cancers in LMIC settings, including non-cervical HPV-associated cancers (anal, oropharyngeal, penile, and vulvar) and conjunctival cancer, with a suspected but as of yet unidentified viral etiology amongst PLWH. Aim 2: Provide centralized virologic and immunologic testing of biospecimens in support of proposed and future PAVILION studies This aim provides centralized viral and immunologic testing of banked specimens in support of proposed and future study aims through a robust set of analytical methodologies across four PAVILION partnering institutions, including LMIC and non-LMIC sites. Aim 3: Provide centralized expert pathology in support of proposed and future PAVILION studies This aim provides comprehensive and expert centralized pathology adjudication of banked tissue specimens in support of the proposed translational studies and future Developmental Core pilot studies.

项目摘要 - 中央实验室核心 中央实验室（CL）核心的总体目标是评估病毒和免疫的合作伙伴关系 与HIV患者（Pavilion）的景观与肿瘤学的交集是为了提供集中式 对良好注释的生物测量的生物群和病毒学和免疫学评估支持 拟议的翻译研究以及未来的馆试点项目。 CL核心将由三个 杰出的调查员，博士。约翰·施耐德（Johann Schneider）（南非），Zvavahera（Mike）Chirenje（Zimbabwe）和 蒂姆·沃特堡（Tim Waterboer）（德国），他们拥有全面的专业知识。 CL共同领导者设计了一个强大的 基于基础设施和长期的柔性管道和分析方法范围 Pavilion合作调查员在南非，津巴布韦建立的国际合作， 美国和德国。 CL核心有目的地将这种独特的专业知识与已建立的实验室配对 低收入和中等收入国家（LMIC）伙伴国家有效地提高与肿瘤和 患者特征。然后，这些知识可以告知有效预防和癌症的发展 LMIC中艾滋病毒（PLWH）患者减少癌症癌症的治疗干预措施 受艾滋病毒的影响不成比例。为了实现总体目标，CL核心将提供集中式， 研究质量的病理评估，以调整收集组织，DNA和RNA提取的终点， HPV和EBV检测和基因分型，RNA表达分析，多重病毒血清学评估以及 拟议项目和未来项目的肿瘤微环境的免疫学评估。这些活动 组织为以下目的： 目标1：在南非和津巴布韦作为集中的生物群资源，用于该收藏 加工和存储生物测量以支持凉亭研究，此目的提供了生物库 在LMIC环境中，包括非颈HPV相关的高负荷和研究不足的病毒癌 癌症（肛门，口咽，阴茎和外阴）和结膜癌，可疑但截至截至 PLWH中未识别的病毒病因。 AIM 2：提供生物测量的集中病毒学和免疫学测试，以支持建议 和未来的凉亭研究，该目标提供了对银行标本的集中病毒和免疫测试 支持拟议的和未来的研究，旨在通过四种强大的分析方法集 Pavilion合作机构，包括LMIC和非LMIC站点。 目标3：提供集中的专家病理学来支持拟议和未来的凉亭研究 AIM提供了全面的专业集中病理病理学调整，对银行组织标本进行调整 支持拟议的翻译研究和未来发展的核心试点研究。