Central Laboratory Core

中心实验室核心

基本信息

批准号：
10598378
负责人：
Johann Wilhelm Schneider
金额：
$ 18.71万
依托单位：
H. LEE MOFFITT CANCER CTR & RES INST
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-08-03 至 2028-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10598378
关键词：
Anus Biological Assay Biological Specimen Banks CDKN2A gene Cancer Burden Cancer Center Characteristics Classification Collaborations Collection Complement Country DNA Databases Development Diagnosis Ensure Etiology Evaluation Future Genetic Transcription Genotype German population Germany Goals Guidelines HIV Histology Human Herpesvirus 4 Human Papilloma Virus-Related Malignant Neoplasm Human Papillomavirus Image Immune Immunofluorescence Immunologic Immunohistochemistry Immunologic Tests Immunologics Infrastructure Institution International Intervention Investigation Knowledge Laboratories Leadership Malignant Neoplasms Methodology NCI Center for Cancer Research Oncology Oropharyngeal Pathologic Pathologist Pathology Patients Persons Pilot Projects Policies Prevention Procedures Process Quality Control RNA Research Research Personnel Resources Sampling Science Serology Site Slide South Africa Specimen Technology Testing Tissue Banks Tissues Tumor-infiltrating immune cells Universities Viral Viral Cancer Vulva Zimbabwe adjudication biobank biological specimen archives cancer therapy design digital experience flexibility low and middle-income countries penis translational study tumor tumor microenvironment viral detection virtual virus related cancer

项目摘要

PROJECT SUMMARY – CENTRAL LABORATORY CORE The overarching goal of the Central Laboratory (CL) Core for the Partnership to Assess Viral and Immune Landscape Intersections with Oncology for People Living with HIV (PAVILION) is to provide centralized biobanking and virologic and immunologic assessment of well annotated biospecimens in support of the proposed translational studies as well as future PAVILION pilot projects. The CL Core will be co-led by three exceptional investigators, Drs. Johann Schneider (South Africa), Zvavahera (Mike) Chirenje (Zimbabwe), and Tim Waterboer (Germany), who have complementary expertise. The CL Co-Leaders have designed a robust yet flexible pipeline and range of analytical methodologies that builds upon the infrastructure and long-term international collaborations established by the PAVILION partnering investigators in South Africa, Zimbabwe, the US, and Germany. The CL Core purposefully pairs this unique expertise with established laboratories in the low- and middle-income country (LMIC) partner countries to efficiently advance knowledge related to tumor and patient characteristics. This knowledge can then inform the development of effective prevention and cancer treatment interventions to reduce the burden of cancer for people living with HIV (PLWH) in LMICs disproportionately impacted by HIV. To achieve the overarching goal, the CL Core will provide centralized, research-quality pathology evaluation for endpoint adjudication of collected tissues, DNA and RNA extraction, HPV and EBV detection and genotyping, RNA expression analysis, multiplex viral serology assessment, and immunological evaluation of the tumor microenvironment for the proposed and future projects. These activities are organized into the following Aims: Aim 1: Serve as a centralized biobanking resource in South Africa and Zimbabwe for the collection, processing, and storage of biospecimens in support of PAVILION studies This aim provides biobanking of high-burden and under-researched viral cancers in LMIC settings, including non-cervical HPV-associated cancers (anal, oropharyngeal, penile, and vulvar) and conjunctival cancer, with a suspected but as of yet unidentified viral etiology amongst PLWH. Aim 2: Provide centralized virologic and immunologic testing of biospecimens in support of proposed and future PAVILION studies This aim provides centralized viral and immunologic testing of banked specimens in support of proposed and future study aims through a robust set of analytical methodologies across four PAVILION partnering institutions, including LMIC and non-LMIC sites. Aim 3: Provide centralized expert pathology in support of proposed and future PAVILION studies This aim provides comprehensive and expert centralized pathology adjudication of banked tissue specimens in support of the proposed translational studies and future Developmental Core pilot studies.

项目摘要——中央实验室核心评估病毒和免疫合作伙伴关系的中央实验室 (CL) 核心的总体目标景观与艾滋病毒感染者肿瘤学交叉点（PAVILION）旨在为艾滋病毒感染者提供集中对注释良好的生物样本进行生物样本库以及病毒学和免疫学评估，以支持拟议的转化研究以及未来的 PAVILION 试点项目将由三个人共同领导。杰出的研究人员，Johann Schneider 博士（南非）、Zvavahera (Mike) Chirenje（津巴布韦）和 Tim Waterboer（德国）拥有互补的专业知识，他们设计了一个强大而强大的系统。灵活的管道和一系列基于基础设施和长期的分析方法 PAVILION 与南非、津巴布韦的调查人员建立的国际合作， CL Core 有目的地将这种独特的专业知识与成熟的实验室结合起来。低收入和中等收入国家 (LMIC) 伙伴国家有效推进与肿瘤和疾病相关的知识这些知识可以为有效预防和癌症的发展提供信息。减轻中低收入国家艾滋病毒感染者 (PLWH) 癌症负担的治疗干预措施为了实现总体目标，CL 核心将提供集中的、研究质量病理学评估，用于对收集的组织、DNA 和 RNA 提取进行终点判定， HPV 和 EBV 检测和基因分型、RNA 表达分析、多重病毒血清学评估以及对拟议和未来项目的肿瘤微环境进行免疫学评估。分为以下目标：目标 1：作为南非和津巴布韦的集中生物样本库资源，收集、处理和存储生物样本以支持 PAVILION 研究这一目标提供了生物样本库中低收入国家中高负担和研究不足的病毒性癌症，包括非宫颈 HPV 相关的癌症癌症（肛门癌、口咽癌、阴茎癌和外阴癌）和结膜癌，疑似但目前尚未发现 PLWH 中病毒病因不明。目标 2：提供生物样本的集中病毒学和免疫学检测，以支持拟议的以及未来的 PAVILION 研究该目标为库存样本提供集中的病毒和免疫学检测通过跨四个领域的一套强大的分析方法来支持拟议的和未来的研究目标 PAVILION 合作机构，包括中低收入国家和非中低收入国家站点。目标 3：提供集中的专家病理学支持拟议的和未来的 PAVILION 研究目标为储存的组织标本提供全面且专家集中的病理学裁决支持拟议的转化研究和未来的发展核心试点研究。