Improving The Scripps Research Institute BSL3 Capabilities to Combat Viruses of Pandemic Concern

提高斯克里普斯研究所 BSL3 对抗流行病病毒的能力

• 批准号: 10611798
• 负责人: Juan C. de la Torre
• 金额: $ 386.96万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10611798
• 关键词: 18 year old; Address; Aging; Air; Air Movements; Animals; Applications Grants; Area; Bunyavirales; COVID-19 pandemic; Cell Separation; Cells; Chromium; Climate; Color; Computer software; Copper; Coronavirus; Data; Devices; Duct (organ) structure; Engineering; Ensure; Equipment; Facility Construction; Faculty; Failure; Filovirus; Flaviviridae; Frequencies; Funding; Future; Gene Expression; Goals; Hamsters; Heating; Housing; Image; Immune; Immunotherapeutic agent; Infrastructure; Leadership; Life Expectancy; Light; Mechanics; Mission; Modernization; Molecular Biology; Mus; Pharmacologic Substance; Procedures; RNA Viruses; Research; Research Activity; Research Institute; Research Personnel; Research Project Grants; Research Support; Resources; Safety; Soil; System; Therapeutic; Time; Togaviridae; Update; Virus; Work; antiviral drug development; biosafety level 3 facility; combat; cost; cost effective; design; digital; exhaust; fluorescence microscope; high efficiency particulate air filter; imaging system; improved; operation; pandemic disease; pandemic preparedness; pressure; programs; rate of change; sensor; single cell analysis; trend; vibration

Project Summary/Abstract The goal of the application “Improving The Scripps Research Institute BSL3 Capabilities To Combat Viruses Of Pandemic Concern” is to renovate and upgrade The Scripps Research Institute (TSRI) current ABSL3 and BSL3 facilities. This will enhance TSRI research capabilities to combat RNA viruses of pandemic potential. TSRI has ~ 2,000 sf dedicated to ABSL3 and ~ 1,500 sf dedicated to BSL3. Currently, these facilities support research projects from eight TSRI faculty whose combined areas of expertise cover research on RNA viruses of pandemic potential, including Bunyavirales, Coronaviridae, Flaviviridae, Filoviridae and Togaviridae. In addition, the facilities are supporting several projects sponsored by pharmaceutical companies. The specific studies have strong translational components aimed at developing antiviral drugs and immunotherapeutics against these RNA viruses. TSRI ABSL3 and BSL3 suites, located in the Molecular Biology Building (MBB), have been operating 24/7 since the last renovations in 2004. The existing mechanical systems have been maintained beyond ASHREA equipment life expectancy for the La Jolla, Ca. coastal climate. In addition, the original design from 1985-87 has resulted in underutilization of ~160 sf in the ABSL3 and ~400 sf in the BSL3. To correct these deficiencies, TSRI has completed a conceptual design with FPBA Architects & tk1sc Mechanical Engineers. This plan includes replacing the aging HVAC systems of the ABSL3 and BSL3 space to increase airflow capacity with N+1 redundancy and update the HEPA filtration system of the ABSL3. In addition, the project will modernize the biocontainment suites by increasing holding capacity (ABSL3), creating a select agent isolation room (BSL3), and installing a new sterilizer (ABSL3). The current ABSL3/BSL3 facilities lack imaging, cell sorting, and single cell analysis capabilities. To remedy these deficiencies, we propose adding to the facility the EVOSTM Imaging System, the SH800S Cell Sorter and the Chromium iX system. These improvements will help to address the increasing demands by TSRI faculty for ABSL3/BSL3 space, time and improved functional capabilities, while ensuring the highest level of safety during daily operations of the facility. Moreover, research activities proposed under the Antiviral Program for Pandemics (APP) U19AI171443 AViDD Grant Application, will greatly benefit from the proposed improvements to the current TSRI ABSL3 and BSL3 facilities. The project will be managed by a well-integrated and highly qualified team, including leaders from Facilities Construction and Engineering, the Institutional Animal Program and Resources, EHS and Biosafety, the Executive VP Office, and investigators directly involved in ABSL3/BSL3 research activities. In addition, the application has the unwavering support of the TSRI leadership.

项目概要/摘要 该应用程序的目标是“提高斯克里普斯研究所 BSL3 对抗病毒的能力” “流行病关注”将翻新和升级斯克里普斯研究所 (TSRI) 现有的 ABSL3 和 BSL3 这将增强 TSRI 对抗具有大流行潜力的 RNA 病毒的研究能力。 约 2,000 平方英尺专用于 ABSL3 和约 1,500 平方英尺专用于 BSL3 目前，这些设施支持研究。 来自 TSRI 八名教员的项目，他们的综合专业领域涵盖大流行性 RNA 病毒的研究 此外，还包括布尼亚病毒目、冠状病毒科、黄病毒科、丝状病毒科和披膜病毒科。 设施正在支持制药公司赞助的几个项目。 强大的翻译成分旨在开发针对这些 RNA 的抗病毒药物和免疫疗法 位于分子生物学大楼（MBB）的 TSRI ABSL3 和 BSL3 套件已在运行。 自 2004 年上次整修以来，24/7 一直保持现有机械系统的维护状态 ASHREA 设备的预期寿命适用于加利福尼亚州拉霍亚沿海气候。 1985-87 年导致 ABSL3 中约 160 平方英尺的利用率不足，BSL3 中约 400 平方英尺的利用率不足。 针对缺陷，TSRI 与 FPBA Architects 和 tk1sc Mechanical Engineers 完成了概念设计。 计划包括更换 ABSL3 和 BSL3 空间老化的 HVAC 系统，以增加气流容量 N+1冗余并更新ABSL3的HEPA过滤系统此外，该项目还将对ABSL3进行现代化改造。 生物防护套件通过增加容纳能力（ABSL3），创建选择代理隔离室（BSL3）， 并安装新的灭菌器 (ABSL3) 目前的 ABSL3/BSL3 设施缺乏成像、细胞分选和单一。 为了弥补这些缺陷，我们建议在该设施中添加 EVOSTM 成像功能。 系统、SH800S 细胞分选仪和 Chromium iX 系统这些改进将有助于解决这个问题。 TSRI 教师对 ABSL3/BSL3 空间、时间和改进的功能能力的需求不断增加，同时 此外，研究建议的活动确保设施日常运营期间的最高安全水平。 根据流行病抗病毒计划（APP）U19AI171443 AViDD拨款申请，将大大受益 该项目将由对当前 TSRI ABSL3 和 BSL3 设施的拟议改进进行管理。 由一支综合性良好、高素质的团队组成，其中包括来自设施建设和工程的领导， 机构动物计划和资源、EHS 和生物安全、执行副总裁办公室和研究人员 直接参与ABSL3/BSL3研究活动此外，该应用程序还得到了坚定不移的支持。 TSRI 领导层。