ROS Analytical Core

ROS 分析核心

• 批准号: 7795675
• 负责人: BHUPENDRA S KAPHALIA
• 金额: $ 12.75万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7795675
• 关键词: Cell membrane; Cells; Chemicals; Collaborations; Core Facility; Generations; Histopathology; Image; Life; Lipid Peroxidation; Lipids; Measurement; Measures; Modeling; Oxidative Stress; Pain; Pathogenesis; Peripheral; Phospholipids; Proteins; Reactive Oxygen Species; Role; Source; Time; Unsaturated Fatty Acids; Work; cell fixing; cellular imaging; central sensitization; functional outcomes; in vivo; oxidation; peroxidation

Core B is the ROS Analytical Core. This is a new core that became necessary because all the projects require quantitative measurement of reactive oxygen species (ROS). Measuring ROS is challenging, particularly when done in vivo, because of multiple intracellular sources of ROS in cells and the known propensity of one form of ROS to readily transform into another. One overriding facet of oxidative stress, however, is peroxidation of unsaturated fatty acid moieties of neutral lipids and phospholipids in cell membranes and oxidation of cellular proteins. Moreover, measuring specific ROS may not explain the functional outcomes in pathological pain and related pathogenesis. An assessment of ROS-induced lipid peroxidation and oxidation of proteins appears to be a logical choice for understanding the underlying mechanism in our pain model. The findings of lipid peroxidation and oxidation proteins will be supported by the real-time live cell and histochemical fixed cell imaging to be conducted in collaboration with Core C. Therefore, ROS Analytical Core will measure lipid peroxidation products and oxidized proteins, and ROS generation in live or fixed cells. The ROS Analytical Core is composed of three branches with an assigned Co-Director for each: 1) Lipid Peroxidation Analysis, 2) Protein Oxidation Analysis, and 3) Histopathology branches. While branches 1) and 2) are for the chemical analysis, branch 3) is to measure intracellular ROS levels using both real-time live-cell imaging and histochemical imaging from fixed cells. For the use of the imaging facility, this core works very closely with Core C (Imaging Core) and the Co-Director of Histopathology of this core is also a Co-Director of the Imaging Core. Therefore, the ROS Analytical Core becomes an integral part of the PPG in delineating the role of ROS in central and peripheral sensitization in pain.

核心 B 是 ROS 分析核心。这是一个必要的新核心，因为所有项目 需要定量测量活性氧 (ROS)。测量 ROS 具有挑战性， 特别是在体内进行时，因为细胞内存在多种 ROS 来源，并且已知 一种形式的 ROS 很容易转化为另一种形式的倾向。氧化应激的一个最重要的方面， 然而，细胞内中性脂质和磷脂的不饱和脂肪酸部分的过氧化反应 膜和细胞蛋白质的氧化。此外，测量特定的 ROS 可能无法解释 病理性疼痛和相关发病机制的功能结果。 ROS诱导脂质的评估 蛋白质的过氧化和氧化似乎是理解其潜在机制的合理选择 我们的疼痛模型中的机制。脂质过氧化和氧化蛋白的发现将得到支持 与 Core C 合作进行实时活细胞和组织化学固定细胞成像。 因此，ROS Analytical Core 将测量脂质过氧化产物和氧化蛋白质，以及 ROS 在活细胞或固定细胞中生成。 ROS 分析核心由三个分支组成，并分配了一个 联席主任：1) 脂质过氧化分析，2) 蛋白质氧化分析，3) 组织病理学 分支机构。分支 1) 和 2) 用于化学分析，分支 3) 用于测量细胞内 ROS 使用实时活细胞成像和固定细胞的组织化学成像来确定水平。为了使用 成像设施，该核心与 Core C（成像核心）和联合主任密切合作 该核心的组织病理学也是成像核心的联合主任。因此，ROS 分析核心 成为 PPG 的一个组成部分，用于描述 ROS 在中枢和外周敏化中的作用 疼痛。