Radiation and Free Radical

辐射和自由基

• 批准号: 10600157
• 负责人: Douglas Robert Spitz
• 金额: --
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-14 至 2026-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10600157
• 关键词: Analytical Chemistry; Antioxidants; Autoradiography; Basic Science; Beta Carotene; Biochemistry; Biological; Biological Assay; Biological Models; Cancer Center Support Grant; Cell Cycle; Cells; Charge; Chemotherapy and/or radiation; Clinical Research; Comprehensive Cancer Center; Couples; DNA; Detection; Doctor of Philosophy; Electron Spin Resonance Spectroscopy; Electron Transport; Environment; Enzymatic Biochemistry; Equipment; Experimental Designs; Free Radicals; Genus Hippocampus; Glutathione Disulfide; High Pressure Liquid Chromatography; Hydrogen Peroxide; Hypoxia; Institution; International; Investigation; Ionizing radiation; Knowledge; Licensure; Lipids; Malignant Neoplasms; Measurement; Measures; Metabolic; Metals; Methodology; Methods; Nitric Oxide; Normal tissue morphology; Oxidants; Oxidation-Reduction; Proteins; Radiation; Radioisotopes; Reactive Oxygen Species; Reagent; Research; Research Personnel; Resources; Safety; Services; Singlet Oxygen; Source; Spectrophotometry; Technology; Testing; Tissues; Translational Research; Translations; Vitamin E; Whole-Body Irradiation; Work; analytical tool; anticancer research; antioxidant enzyme; ascorbate; cell behavior; clinical translation; cost; dosimetry; experience; experimental analysis; experimental study; instrumentation; member; operation; overexpression; oxidation; oxidative damage; pre-clinical; quality assurance; radiation response; radiotracer; response; small molecule; theranostics; tissue injury; tool; tumor

The Radiation Free Radical Research Core (RFRRC) facilitates Holden Comprehensive Cancer Center (HCCC) researcher investigations that require radiation services or quantitative methods for analyzing and manipulating redox status and redox based biological changes. It serves both HCCC members and cancer researchers at other institutions. The RFRRC has been in operation since 1947, and is directed by Douglas Spitz, PhD; Frederick Domann, Jr, PhD; Garry Buettner, PhD; and Prabhat Goswami, PhD all of whom have extensive experience and international reputations in free radical-focused cancer research. The RFRRC provides three basic services. Ionizing radiation services are supplemented by phosphorimaging and cell-cycle analytical tools critical to understanding basic cellular behavior and responses to radiation and chemotherapy. Electron paramagnetic resonance spectroscopy and other analytical chemistry detection methodologies are available for measuring free radicals, singlet oxygen, small molecule antioxidants, nitric oxide and the array of related oxidants and oxidative damage products. Antioxidant enzyme services provide easy access to technologies for modifying and measuring molecules responsible for pro-oxidant formation and oxidative damage. Major equipment available in the RFRRC includes several radiation sources, a Seahorse analyzer, various types of spectrophotometers, hypoxia chambers, and equipment for HPLC separation and analyte detection. The RFRRC provides HCCC members with easy access to specialized knowledge, reagents, equipment and resources in a highly collaborative and helpful environment.

辐射自由基研究核心 (RFRRC) 促进霍尔顿综合癌症中心 (HCCC) 需要辐射服务或定量方法进行分析的研究人员调查 以及操纵氧化还原状态和基于氧化还原的生物变化。它为 HCCC 会员和 其他机构的癌症研究人员。 RFRRC 自 1947 年起开始运作，其职责是 作者：道格拉斯·斯皮茨博士；弗雷德里克·多曼，Jr.，博士；加里·比特纳博士；和普拉巴特·戈斯瓦米， 博士所有人在自由基癌症领域拥有丰富的经验和国际声誉 研究。 RRRRC 提供三项基本服务。电离辐射服务的补充包括 磷光成像和细胞周期分析工具对于理解基本细胞行为和 对放疗和化疗的反应。电子顺磁共振波谱等 分析化学检测方法可用于测量自由基、单线态氧、 小分子抗氧化剂、一氧化氮以及一系列相关氧化剂和氧化损伤 产品。抗氧化酶服务可轻松获取修饰和抗氧化酶技术 测量负责促氧化剂形成和氧化损伤的分子。主要设备 RFRRC 中提供的包括多种辐射源、海马分析仪、各种类型的 分光光度计、缺氧室以及 HPLC 分离和分析物检测设备。 RFRRC 为 HCCC 成员提供轻松获取专业知识、试剂、 高度协作和有益的环境中的设备和资源。