Mouse Phenotype Core, Project 9 of 10

小鼠表型核心，项目 9（共 10 个）

• 批准号: 8139678
• 负责人: DAVID W. RUSSELL
• 金额: $ 37.08万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-30 至 2013-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8139678
• 关键词: Affect; Amino Acid Sequence; Animal Disease Models; Animal Model; Antibody Formation; Antigens; Arts; Behavioral; Behavioral Assay; Biological Assay; Blood Chemical Analysis; Brain; Collaborations; Consult; Core Facility; Crystallography; DNA Sequence; Data; Detection; Disease; Electronics; Ensure; Equilibrium; Equipment; Euglycemic Clamping; Faculty; Fee-for-Service Plans; Flow Cytometry; Funding; Gene Expression; Gene Targeting; Glucose; Glucose Clamp; Goals; Grant; Guidelines; Health Services Accessibility; Histology; Home environment; Hormones; Image; In Situ; Kinetics; Knowledge; Laboratories; Life; Lipids; Magnetic Resonance; Mass Spectrum Analysis; Measurement; Messenger RNA; Metabolic; Methodology; Methods; Microarray Analysis; Mission; Mus; Neuroanatomy; Obesity; Organism; Patient Care; Peptide Sequence Determination; Phenotype; Physiological; Physiology; Procedures; Production; Quality Control; RNA chemical synthesis; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Running; Sampling; Scientist; Serum; Services; Slice; Staging; Staining method; Stains; Study of magnetics; Synaptic plasticity; Testing; Time; Transgenic Animals; Translating; Work; X-Ray Computed Tomography; adverse outcome; analytical ultracentrifugation; base; body system; cellular imaging; glucose tolerance; indexing; insight; interest; laser capture microdissection; member; novel; research study; small molecule; structural biology; success; web site

The adverse consequences of obesity are pleiotrophic for the mammalian organism. Animal models of obesity that allow careful characterization of the pervasive metabolic effects of the disease are vital to the success of our mission. To enhance research into the causes and consequences of obesity, we established a mouse phenotyping core. The primary goals of this core are to develop and provide standardized state-ofthe- art methods for the analysis of animal models of disease. The core applies the analytical expertise of ten laboratories to provide histology, neuroanatomy, gene expression, mass spectrometry, physiology, lipid, glucose, metabokine, and behavioral assays. The core is directed by Russell, Elmquist, and Scherer and is staffed by a dedicated team of skilled technicians who utilize existing equipment to perform optimized assays on samples provided by TORS investigators. Currently available assays include histological staining and antigen detection, in situ mRNA hybridization, real time reverse transcriptase-PCR, microarray analysis, laser capture microdissection, small molecule mass spectrometry and metabolite profiling, metabolic cage analyses, complete lipid balance studies, magnetic resonance and computed tomography imaging, glucose tolerance and clamp analyses, serum hormone measurements, routine blood chemistries, exhaustive behavioral analyses, and synaptic plasticity measurements in brain slices. As new research findings dictate, novel methodologies are developed and made available by the core. For each experiment, results are collected, calculated, and compiled in electronic format and returned to submitting investigators. The availability of the services provided by this core accelerates the pace of our research, allows centralized troubleshooting, and facilitates the efficient utilization of existing expertise and resources. The mouse phenotyping core is a crucial component of our Roadmap effort that brings together a diverse team of scientists and clinicians to ensure that discoveries made in obesity research are rapidly translated into advances in patient care.

肥胖的不利后果对于哺乳动物来说是多效性的。动物模型 肥胖症可以仔细表征疾病的普遍代谢影响，这对于 我们的使命取得成功。为了加强对肥胖原因和后果的研究，我们建立了 小鼠表型核心。该核心的主要目标是开发和提供标准化的最新技术 用于分析疾病动物模型的艺术方法。核心应用了十位专家的分析专业知识 实验室提供组织学、神经解剖学、基因表达、质谱、生理学、脂质、 葡萄糖、代谢因子和行为测定。核心由 Russell、Elmquist 和 Scherer 执导， 拥有一支由熟练技术人员组成的专业团队，他们利用现有设备来执行优化的检测 由 TORS 调查员提供的样本。目前可用的检测方法包括组织学染色和 抗原检测、原位 mRNA 杂交、实时逆转录酶 PCR、微阵列分析、 激光捕获显微切割、小分子质谱和代谢物分析、代谢笼 分析、完整的脂质平衡研究、磁共振和计算机断层扫描成像、葡萄糖 耐受性和钳夹分析、血清激素测量、常规血液化学、详尽的 行为分析和大脑切片中的突触可塑性测量。新的研究结果表明， 核心开发并提供了新的方法。对于每个实验，结果是 以电子格式收集、计算和汇编并返回给提交调查人员。这 该核心提供的服务的可用性加快了我们研究的步伐，允许集中 故障排除，并促进现有专业知识和资源的有效利用。鼠标 表型分析核心是我们路线图工作的重要组成部分，该路线图汇集了多元化的团队 科学家和临床医生确保肥胖研究的发现能够迅速转化为 患者护理方面的进步。

项目成果

Plasma Leptin Levels and Risk of Incident Cancer: Results from the Dallas Heart Study.

血浆瘦素水平和发生癌症的风险：达拉斯心脏研究的结果。

• 发表时间: 2016
• 影响因子: 3.7
• 作者: Gupta, Arjun;Herman, Yehuda;Ayers, Colby;Beg, Muhammad S;Lakoski, Susan G;Abdullah, Shuaib M;Johnson, David H;Neeland, Ian J
• 通讯作者: Neeland, Ian J

Relation of regional fat distribution to left ventricular structure and function.

区域脂肪分布与左心室结构和功能的关系。

• 发表时间: 2013-09
• 作者: Neeland, Ian J;Gupta, Sachin;Ayers, Colby R;Turer, Aslan T;Rame, J Eduardo;Das, Sandeep R;Berry, Jarett D;Khera, Amit;McGuire, Darren K;Vega, Gloria L;Grundy, Scott M;de Lemos, James A;Drazner, Mark H
• 通讯作者: Drazner, Mark H

The expression of MC4Rs in D1R neurons regulates food intake and locomotor sensitization to cocaine.

D1R 神经元中 MC4R 的表达调节食物摄入和对可卡因的运动敏感性。

• 发表时间: 2013-08
• 作者: Cui, H;Lutter, M
• 通讯作者: Lutter, M

Ire1α in Pomc Neurons Is Required for Thermogenesis and Glycemia.

Pomc 神经元中的 Ire1α 是生热和血糖所必需的。

• 发表时间: 2017
• 影响因子: 7.7
• 作者: Yao, Ting;Deng, Zhuo;Gao, Yong;Sun, Jia;Kong, Xingxing;Huang, Yiru;He, Zhenyan;Xu, Yanchao;Chang, Yongsheng;Yu, Kai;Findley, Brianna G;Berglund, Eric D;Wang, Rui;Guo, Hongbo;Chen, Hong;Li, Xu;Kaufman, Randal J;Yan, Jianqun;Liu
• 通讯作者: Liu

Cannabinoid receptor 1 in the vagus nerve is dispensable for body weight homeostasis but required for normal gastrointestinal motility.

迷走神经中的大麻素受体 1 对于体重稳态是可有可无的，但对于正常的胃肠道运动是必需的。

• 发表时间: 2012-07-25
• 作者: Vianna, Claudia R;Donato Jr, Jose;Rossi, Jari;Scott, Michael;Economides, Kyriakos;Gautron, Lauren;Pierpont, Stephanie;Elias, Carol F;Elmquist, Joel K
• 通讯作者: Elmquist, Joel K