Host Microbe Core

宿主微生物核心

基本信息

批准号：
10237340
负责人：
Lucas R Hoffman
金额：
$ 20.73万
依托单位：
SEATTLE CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-06 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10237340
关键词：
Address Adverse effects Animal Model Animals Antibiotics Area Attention Bacteria Bacteroides Biochemical Biological Assay Biology Cells Cellular biology Clinical Clinical Microbiology Clinical Research Collaborations Communities Consultations Cystic Fibrosis Cystic Fibrosis Transmembrane Conductance Regulator Disease Disease Outcome Endocrine Endocrine system Epithelial Equipment Escherichia coli Evolution Experimental Designs Fostering Functional disorder Gastrointestinal tract structure Genomics Goals Growth Histocytochemistry Histopathology Facility Image Immune Immune response Immunohistochemistry In Situ Hybridization In Vitro Inflammation Inflammatory Inflammatory Response Infrastructure Investigation Kidney Laboratories Life Expectancy Link Liver Lung Malignant Neoplasms Measures Methods Microbe Microbiology Microscopy Morbidity - disease rate Mucous Membrane National Institute of Diabetes and Digestive and Kidney Diseases Organ Outcome Pancreas Pathogenesis Pathologic Pathology Pharmaceutical Preparations Phenotype Pilot Projects Proteome Proteomics Reagent Renal function Reproducibility Research Research Personnel Research Support Resources Respiratory System Role Sampling Services Slide Specialist Specimen Suggestion Surface Techniques Tissues Training Translational Research Work airway epithelium base behavioral study body system bone clinically relevant cystic fibrosis patients design digital imaging digital pathology digital repositories experience experimental study gastrointestinal gut microbiota improved innovation interest metabolome microbial microbial host microbiome microbiome components next generation novel pathogen pre-clinical research programs ranpirnase targeted imaging tool transcriptome translational approach treatment response vaccine response

项目摘要

ABSTRACT The Host Microbe Core (HMC) will assist and support the research base, trainees, and pilot project investigators by providing specimens, reagents, training, and services to define both the microbial and host response determinants of CF diseases in organs and tissues prioritized by the NIDDK. This Core combines the resources and expertise of specialists in CF basic and clinical microbiology, airway epithelial and immune cell biology, pathology, and microscopy to provide an integrated network of services, reflecting an evolution in the field of CF disease pathogenesis to concurrently investigate the host and pathogens. Core services available to the UW CF P30 research base include: 1. Technical assistance and state-of-the-art facilities for histopathology, immunohistochemistry, in situ hybridization and localization of host and microbial targets, and digital imaging 2. Quantitative digital pathology and whole slide digital repository for pancreata and lungs from people with CF 3. Processing clinical, animal, and laboratory specimens for microbiome, proteome, metabolome, and transcriptome analyses, and for specific targets 4. Microbial culturing of CF specimens in formats representative of CF mucosal conditions for phenotypic, proteomic, genomic, and biochemical analyses 5. Access to strains of bacteria cultured from CF clinical fecal samples, including Escherichia coli and Bacteroides spp. 6. Consultation and training in methods provided by the Core and experimental design 7. Interacting closely with the Clinical and Genomics cores to provide unified services for CF researchers

抽象的主机微生物核心（HMC）将协助和支持研究基础，受训者和试点项目通过提供标本，试剂，培训和服务来定义微生物和宿主的调查人员 NIDDK优先考虑器官和组织中CF疾病的反应决定因素。这个核心结合了 CF基本和临床微生物学，气道上皮和免疫细胞专家的资源和专业知识生物学，病理学和显微镜提供了综合服务网络，反映了 CF疾病发病机理的领域，同时研究宿主和病原体。可用的核心服务到UW CF P30研究基础包括： 1。原位，免疫组织化学组织病理学的技术援助和最先进的设施宿主和微生物靶标的杂交和定位以及数字成像 2。胰腺和肺部的定量数字病理学和整个幻灯片数字存储库与CF 3。处理微生物组，蛋白质组，代谢组的临床，动物和实验室标本转录组分析和针对特定目标 4。代表表型，代表CF粘膜条件的格式中CF样品的微生物培养蛋白质组学，基因组和生化分析 5。从CF临床粪便样品中培养的细菌菌株，包括大肠杆菌和细菌属。 6。核心和实验设计提供的方法的咨询和培训 7。与临床和基因组核紧密互动，为CF研究人员提供统一的服务