Shared Resource-Flow Cytometry Core

共享资源-流式细胞术核心

• 批准号: 10560570
• 负责人: Ye Zheng
• 金额: $ 19.63万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-31 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10560570
• 关键词: Accounting; Animal Model; Antibodies; Apoptosis; Cancer Center; Cancer Center Support Grant; Cell Cycle Progression; Cell Separation; Cells; Computer software; Consultations; Data Analyses; Dedications; Detection; Education; Ensure; Experimental Designs; Flow Cytometry; Flow Cytometry Shared Resource; Fluorescent Antibody Technique; Fostering; Immune; Label; Licensing; Phenotype; Ploidies; Population; Proteins; Protocols documentation; Research; Research Personnel; Resource Sharing; Resources; Sampling; Scientist; Services; Site; Techniques; Technology; Training; assay development; cancer cell; cancer type; cell type; collaborative environment; design; experience; experimental study; fluorescence activated cell sorter device; instrument; instrumentation; member; new technology; operation; research and development; trend; tumor microenvironment

Flow Cytometry Core Shared Resource - Project Summary/Abstract The Flow Cytometry Core provides Salk investigators access to state-of-the-art flow cytometry instrumentation, high quality services (ensuring that all samples are manipulated and analyzed appropriately), and expert consultation concerning the design and implementation of flow-based experiments. The facility is equipped with two staff-operated fluorescence activated cell sorters (FACS) and three analytical instruments that are available for independent operation. Training and support for users with all levels of experience are available at the Core. The Core manages a site license for data analysis software, and provides users with two analysis workstations. Flow cytometry is a powerful single-cell technique that is widely employed at the Salk Institute, with Cancer Center members accounting for 95% of overall usage. Examples of flow applications used by Cancer Center members include: 1) characterizing cellular phenotypes (e.g., DNA content, levels and rates of cell cycle progression, levels of apoptosis), 2) using fluorescent proteins to label, identify, and FACS purify specific types of cancer cells from animal models, and 3) using fluorescent antibody labeling approaches to resolve multiple cell types (e.g., to determine which populations of cells, including immune cells, are present within the tumor microenvironment). This latter technique is applied to an extremely diverse array of experiments, which is limited only by antibody availability and detection real estate. The facility staff fosters a collaborative environment, striving to provide an easily accessible comprehensive resource to the Institute and dedicates efforts to identify emerging flow technologies or applications that will be useful for Core users. The specific aims of this Shared Resource are to facilitate research by: 1) offering instrumentation – the Core maintains a suite of instruments designed to meet the broad range of current and anticipated needs of Institute scientists (based on research developments and trends), 2) providing expertise – the highly experienced Core staff is readily accessible to Salk investigators for the collaborative development of assays and/or protocols, 3) providing critical services – Core staff provides high-quality, live cell-sorting services that are tailored to meet the varying needs of Institute scientists, 4) providing education, training, and support – Salk researchers at all levels of expertise can receive training in all aspects of flow cytometry, from experimental design to data analysis, and 5) identifying and evaluating new technologies – the Core seeks to continuously ensure that state-of-the-art flow capabilities are offered, thereby maximizing user benefit.

流式细胞术核心共享资源 - 项目摘要/摘要 流式细胞术核心为索尔克研究人员提供了最先进的流式细胞术仪器， 高质量的服务（确保所有样本都得到适当的操作和分析）和专家 有关基于流程的实验的设计和实施的咨询 该设施配备齐全。 配备两台工作人员操作的荧光激活细胞分选仪 (FACS) 和三台分析仪器 可以为具有各种经验水平的用户提供独立操作的培训和支持。 Core 管理数据分析软件的站点许可证，并为用户提供两种分析。 流式细胞术是索尔克研究所广泛采用的一种强大的单细胞技术， 癌症中心会员占总体使用量的 95%。 癌症中心成员包括：1) 表征细胞表型（例如，DNA 含量、水平和比率） 细胞周期进展、细胞凋亡水平），2）使用荧光蛋白进行标记、识别和 FACS 纯化 来自动物模型的特定类型的癌细胞，以及 3) 使用荧光抗体标记方法 解析多种细胞类型（例如，确定存在哪些细胞群，包括免疫细胞 肿瘤微环境内）。后一种技术适用于极其多样化的阵列。 实验，仅受抗体可用性和检测空间的限制。设施工作人员培养了一种方法。 协作环境，努力为研究所提供易于访问的综合资源 致力于识别对核心用户有用的新兴流技术或应用程序。 该共享资源的具体目标是通过以下方式促进研究：1）提供仪器——核心 拥有一套旨在满足研究所当前和预期的广泛需求的仪器 科学家（基于研究进展和趋势），2）提供专业知识——经验丰富的核心 索尔克研究人员可以随时与工作人员联系，以协作开发分析和/或协议，3) 提供关键服务——核心人员提供高质量的活细胞分选服务，以满足 研究所科学家的不同需求，4) 提供教育、培训和支持——索尔克研究人员 专业水平可以接受流式细胞术各个方面的培训，从实验设计到数据 分析，以及 5) 识别和评估新技术——核心力求不断确保 提供最先进的流量功能，从而最大限度地提高用户利益。