Core E Research Support

核心E研究支持

• 批准号: 10560522
• 负责人: RUI-MING LIU
• 金额: $ 7.76万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-15 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10560522
• 关键词: Adult; Affect; African American; Air; Animal Model; Animals; Annual Reports; Area; Arsenic; Asthma; Authorization documentation; Bioinformatics; Biological; Biological Process; Bronchoalveolar Lavage; Cadmium; Chronic; Chronic Obstructive Pulmonary Disease; Clinical; Clinical Data; Clinical Research; Collaborations; Communities; County; Data Analyses; Data Collection; Databases; Dust; Eligibility Determination; Enrollment; Ensure; Environment; Environmental Exposure; Exposure to; Future; Goals; Heavy Metals; High Prevalence; Human; Human Resources; IACUC; Individual; Industrialization; Informed Consent; Infrastructure; Inhalation; Inhalation Exposure; Institutional Review Boards; International; Knowledge acquisition; Laboratories; Life; Lower Respiratory Tract Infection; Lung diseases; Lung infections; Manganese; Metal exposure; Methods; Mission; Modeling; Mus; National Institute of Environmental Health Sciences; Plasma; Poison; Pollution; Population; Postdoctoral Fellow; Procedures; Process; Protocols documentation; Public Health; Quality Control; Research; Research Personnel; Research Support; Resources; Respiratory Tract Infections; Role; Safety; Sampling; Secure; Services; Signal Recognition Particle; Site; Societies; Specimen; Specimen Handling; Standardization; Superfund; Tissue Harvesting; Training; Translational Research; United States; Vertebrates; aerosolized; animal facility; biobank; clinical center; community engagement; data management; experience; human subject; insight; lung injury; mouse model; organizational structure; programs; recruit; sample collection; sex; success; synergism

ABSTRACT Due to decades of heavy industrial activity, the North Birmingham area has been severely polluted with toxic chemicals including heavy metals such as cadmium, arsenic, and manganese. The population in the proposed North Birmingham National Priorities List (NPL) site (Affected Areas; zip codes 35207 and 35217) is predominantly African American and has higher rates of chronic obstructive pulmonary disease (COPD), lower respiratory tract infection (LRTI), and asthma compared to less industrialized areas (Control Area) of Jefferson County (zip code 35214). Considering the high prevalence of lung disease in the United States, particularly COPD, asthma, and respiratory tract infections, the proposed research is relevant to public health. The Clinical Biorepository and Animal Exposure Core (Core E) has two specific aims: Specific Aim 1: Manage the identification, recruitment, informed consent process, enrollment, clinical data collection, and biological specimen collection from subjects living in the Affected and Control Areas; Specific Aim 2: Expose adult mice (C57BL/6) of both sexes to arsenic, manganese, cadmium or real-life filtered dust from Affected and Control Areas for 4-6 hrs per day for three weeks; mice will be transferred to various projects so they can complete their proposed specific aims. The activities of the Clinical Biorepository and Animal Exposure Core will be essential to the overall success of this Superfund Program. The comprehensive biorepository of human specimens would constitute a unique resource that would further the NIEHS/Superfund mission. Furthermore, exposing mice to heavy metals in concentrations that are present in ambient air will create a model that mirrors and recapitulates the environmental exposures at the NPL site. The Core will be comprised of a pulmonologist with ample experience in clinical research, a toxicologist with extensive experience in mouse inhalation and lung injury study, a research coordinator, and a research associate. Core activities will be closely integrated and supported by the Data Management and Analysis Core (DMAC), Community Engagement Core (CEC), the biorepository infrastructure of the UAB Center for Clinical Translational Science (CCTS), and the Inhalation Exposure Facility within the UAB Animal Facility. All research personnel will be required to fulfill and maintain the training requirements of the UAB IRB and the IACUC. The IRB and the IACUC will review and provide oversight of all research-related activities. Procedures will be in place to ensure safety of human subjects and humane handling of vertebrate animals. The UAB CCTS Biorepository follows best practices defined by international societies to ensure that validated and reliable procedures and protocols are in place for specimen handling and sample integrity. The centralized, standardized and comprehensive expertise and organization of this Core represents a value-based, economy of effort for the overall proposed center.

抽象的 由于数十年的重型工业活动，北伯明翰地区已受到严重污染 有毒化学物质，包括重金属，例如镉，砷和锰。人口 拟议的北伯明翰国家优先事项列表（NPL）地点（受影响地区；邮政编码35207和35217）是 主要是非裔美国人 与杰斐逊的工业化区域（控制区）相比，呼吸道感染（LRTI）和哮喘 县（邮政编码35214）。考虑到美国，尤其是肺部疾病的高患病率 COPD，哮喘和呼吸道感染，拟议的研究与公共卫生有关。临床 生物座和动物暴露核心（核心E）有两个具体的目的：特定目的1：管理 识别，招聘，知情同意过程，注册，临床数据收集和生物标本 来自居住在受影响和控制区域的受试者的收集；特定目标2：暴露成年小鼠（C57BL/6） 两性到砷，锰，镉或现实生活中的粉尘，来自受影响和控制区域的灰尘，4-6 每天HRS三周；小鼠将被转移到各种项目中，以便他们可以完成他们的建议 具体目标。临床生物疗法和动物暴露核心的活动对于整体至关重要 这个超级基金计划的成功。人类标本的综合生物座将构成 独特的资源将进一步执行NIEHS/SUPERFUND任务。此外，将老鼠暴露于重金属 在环境空气中存在的浓度中，将创建一个模型，以反映和概括 NPL站点的环境暴露。核心将由具有丰富经验的肺科医生组成 在临床研究中，一名具有丰富小鼠吸入和肺损伤研究经验的毒理学家，一项研究 协调员和研究助理。核心活动将由数据密切集成和支持 管理与分析核心（DMAC），社区参与核心（CEC），生物座基础架构 UAB临床转化科学中心（CCTS）和UAB内的吸入暴露设施 动物设施。所有研究人员都必须满足和维护UAB的培训要求 IRB和IACUC。 IRB和IACUC将审查并监督所有与研究相关的活动。 将制定程序，以确保人类受试者的安全和人道处理脊椎动物。这 UAB CCTS Biorepository遵循国际社会定义的最佳实践，以确保已验证和 可靠的程序和协议已适当用于样品处理和样本完整性。集中的 标准化和全面的专业知识和该核心的组织代表了基于价值的经济 整体建议中心的努力。