Antimicrobial Peptides as Probes for Electrochemical Detection of Methicillin-resistant Staphylococcus Aureus

抗菌肽作为电化学检测耐甲氧西林金黄色葡萄球菌的探针

• 批准号: 10271841
• 负责人: Rebecca Y Lai
• 金额: $ 11.15万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-15 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10271841
• 关键词: Address; Admission activity; Affinity; Antibodies; Area; Attention; Biological; Biological Assay; Biosensing Techniques; Buffers; Carbon; Cells; Chemistry; Collaborations; Communication; Communication Research; Consumption; Detection; Development; Devices; Diagnosis; Disease; Early identification; Electrochemistry; Electrodes; Elements; Ensure; Future; Generations; Genus staphylococcus; Goals; Gold; Health care facility; Healthcare Systems; Hospitals; Immunoassay; Infection; Ions; Knowledge; Length; Length of Stay; Life; Medical center; Metals; Methods; Microfluidic Microchips; Microfluidics; Modification; Morbidity - disease rate; Multi-Drug Resistance; Nebraska; Nosocomial Infections; Outcome; Paper; Patients; Peptide Fragments; Peptides; Polymerase Chain Reaction; Property; Proteins; Reaction; Reaction Time; Reagent; Research; Resources; Saliva; Sampling; Spectrum Analysis; Staphylococcus aureus; Staphylococcus aureus infection; Surface Plasmon Resonance; Technology; Testing; Time; Universities; antimicrobial peptide; base; cost; cost effective; design; detection limit; detection method; disease diagnosis; healthcare-associated infections; innovation; methicillin resistant Staphylococcus aureus; mortality; nasal swab; novel; resistant strain; sensor

PROJECT SUMMARY/ABSTRACT The broad, long-term goal of Project Leader Rebecca Lai’s project is to develop a near real-time method for detecting methicillin-resistant Staphylococcus aureus (MRSA). In recent decades, MRSA strains have become the most common cause of health care-associated infections, and new multi-drug-resistant strains of this severe and potentially life-threatening disease have emerged. Although implementation of universal admission surveillance can reduce the spread of MRSA infections, it is not practiced in many health care systems because of the cost of testing. Thus, there is a critical need for more advanced detection methods that are sensitive, specific, and rapid yet cost-effective enough to be employed in hospitals and health care facilities in low resource settings. An area that deserves attention in MRSA sensor design is the selection of appropriate biorecognition elements. Among available biorecognition elements, antimicrobial peptides (AMPs), which are short peptide fragments existing in various forms of life, are one of the best alternatives to antibodies because of a combination of attractive properties: high stability, synthetic simplicity, easy accessibility, and high affinity toward their specific bacterial targets. Thus, the goal of this project is to evaluate and characterize MRSA-specific peptides suitable for use as biorecognition elements in the fabrication of E-PB MRSA sensors and to use potential-assisted “click” chemistry in the construction of a paper-based sensor array. Two aims will be achieved: 1) design and fabricate E-PB sensors using high efficacy AMPs for detection of MRSA and 2) develop a paper-based E-PB sensor array using potential-assisted “click” chemistry. To ensure that the sensor array will perform optimally, critical aspects of the bioconjugation reaction will be systematically evaluated, including the reaction time, applied potential, and concentrations of the reagents. This project will result in an E-PB sensor array capable of near real-time detection of MRSA bacterial cells, which will be an important step toward the development of an all-in-one microfluidic- based MRSA sensor device. Project outcomes will stimulate future research on AMPs for biosensing applications. The availability of such a versatile sensing platform could have a major impact on MRSA diagnosis, management, and surveillance. This project will also contribute to the wider goal of the Nebraska Center for Integrated Biomolecular Communication (CIBC), which includes the development of novel biomolecular sensing strategies for disease diagnosis. It will enhance CIBC’s research strengths in biosensing and bioanalysis and facilitate future development of sensors for other important diseases.

项目概要/摘要 项目负责人 Rebecca Lai 项目的广泛、长期目标是开发一种近乎实时的方法 检测耐甲氧西林金黄色葡萄球菌 (MRSA) 近几十年来，MRSA 菌株已成为一种耐药菌株。 医疗保健相关感染的最常见原因，以及这种严重疾病的新的多重耐药菌株 尽管普遍入院，但已经出现了潜在危及生命的疾病。 监测可以减少 MRSA 感染的传播，但许多医疗保健系统并未实施这种做法，因为 因此，迫切需要更先进的、灵敏的检测方法。 具体、快速且具有成本效益，足以在资源匮乏的医院和医疗保健机构中使用 MRSA 传感器设计中值得关注的一个领域是选择适当的生物识别。 在可用的生物识别元件中，抗菌肽（AMP）是短肽。 存在于各种生命形式中的片段，由于其组合而成为抗体的最佳替代品之一 具有吸引力的特性：高稳定性、合成简单、易于获取以及对其特定的高亲和力 因此，该项目的目标是评估和表征合适的 MRSA 特异性肽。 在 E-PB MRSA 传感器的制造中用作生物识别元件并使用电位辅助“点击” 化学在纸基传感器阵列的构建中将实现两个目标：1）设计和制造。 E-PB 传感器使用高效 AMP 检测 MRSA，2) 开发纸基 E-PB 传感器阵列 使用电位辅助“点击”化学来确保传感器阵列在关键方面发挥最佳性能。 将系统地评估生物共轭反应的过程，包括反应时间、施加的电位和 该项目将产生能够近乎实时检测的 E-PB 传感器阵列。 MRSA 细菌细胞，这将是开发一体化微流体的重要一步 基于 MRSA 传感器设备的项目成果将刺激未来对 AMP 生物传感的研究。 这种多功能传感平台的可用性可能会对 MRSA 诊断产生重大影响。 该项目还将有助于实现内布拉斯加州中心的更广泛目标。 集成生物分子通信 (CIBC)，包括新型生物分子传感的开发 它将增强 CIBC 在生物传感和生物分析方面的研究实力。 促进其他重要疾病传感器的未来开发。